Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 116318
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse EMMPRIN/CD147
Ala22-Arg209
Accession # NP_001070652

Specificity

Detects mouse EMMPRIN/CD147 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human EMMPRIN is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse EMMPRIN/CD147 Antibody (116318)

Detection of bntw9ok6eutxni21387t.jpg by Immunohistochemistry

Detection of bntw9ok6eutxni21387t.jpg by Immunohistochemistry

161-MAP active vaccination reduces EMMPRIN expression in the colon. (A) Representative images of colon sections stained for EMMPRIN and their quantification using the H-score (n = 5 per group). Scale bar is 25 μm (B) Representative image of fluorescently labeled EMMPRIN (red) and macrophages (green). Scale bar is 100 μm. (C) Determination of EMMPRIN concentrations in colon lysates (n = 9–10 per group), and in serum samples (n = 8–9 per group) by ELISA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30619283), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of EMMPRIN/CD147 by Immunohistochemistry

Detection of EMMPRIN/CD147 by Immunohistochemistry

161-MAP active vaccination reduces EMMPRIN expression in the colon. (A) Representative images of colon sections stained for EMMPRIN and their quantification using the H-score (n = 5 per group). Scale bar is 25 μm (B) Representative image of fluorescently labeled EMMPRIN (red) and macrophages (green). Scale bar is 100 μm. (C) Determination of EMMPRIN concentrations in colon lysates (n = 9–10 per group), and in serum samples (n = 8–9 per group) by ELISA. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/30619283), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse EMMPRIN/CD147 Antibody (116318)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse EMMPRIN/CD147 Fc Chimera

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: EMMPRIN/CD147

EMMPRIN, also known as basigin and CD147, is a type I membrane protein belonging to the immunoglobulin superfamily.

Long Name

Extracellular Matrix Metalloproteinase Inducer

Alternate Names

Basigin, BSG, CD147

Entrez Gene IDs

682 (Human); 12215 (Mouse); 102121721 (Cynomolgus Monkey)

Gene Symbol

BSG

UniProt

Additional EMMPRIN/CD147 Products

Product Documents for Mouse EMMPRIN/CD147 Antibody (116318)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Mouse EMMPRIN/CD147 Antibody (116318)

For research use only

Citations for Mouse EMMPRIN/CD147 Antibody (116318)

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