Mouse EphA6 Antibody

Catalog # Availability Size / Price Qty
MAB6071
MAB6071-SP
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Mouse EphA6 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse EphA6 in direct ELISAs and Western blots. In direct ELISAs, approximately 15% cross‑reactivity with recombinant rat EphB1 is observed and no cross-reactivity with recombinant human EphA1, A5, recombinant mouse EphA2, A3, A4, A7, A8, B2, B3, B4, or B6 is observed.
Source
Monoclonal Rat IgG1 Clone # 81605
Purification
Protein A or G purified from ascites
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse EphA6
Met1-Gln546
Accession # Q62413
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse EphA6 Fc Chimera (Catalog # 607-A6)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 0.05-0.15 µg/mL of this antibody will block 50% of the binding of 20 ng/mL of biotinylated Recombinant Human Ephrin A3 to immobilized Recombinant Mouse EphA6 Fc Chimera (Catalog # 607-A6) coated at 2 µg/mL (100 µL/well). At 1 μg/mL, this antibody will block >90% of the binding.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: EphA6

EphA6, also known as Ehk2 and Hek12 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphA6 has been shown to bind ephrin-A2, ephrin-A1, ephrin-A3, ephrin-A4, and ephrin-A5 (2, 3). The extracellular domains of rat and mouse EphA6 share 99% amino acid identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/ephrin families also appear to play a role in angiogenesis (3).

Long Name
Eph Receptor A6
Entrez Gene IDs
285220 (Human); 13840 (Mouse)
Alternate Names
DKFZp434C1418; EC 2.7.10; EC 2.7.10.1; Ehk2; EHK-2; EPA6; EPH homology kinase 2; EPH receptor A6; EphA6; ephrin receptor EphA6; ephrin type-A receptor 6; FLJ35246; Hek12; PRO57066

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