Mouse Ephrin-A2 Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Ephrin-A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Ephrin-A1, recombinant human (rh) Ephrin-A3, rmEphrin-A4, rhEphrin-A5, rmEphrin-B1, rmEphrin-B2, and rhEphrin-B3 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse Ephrin-A2
    Met1-Asn184
    Accession # P52801
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2)
  • Blockade of Receptor-ligand Interaction
    In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2) to immobilized Recombinant Rat EphA5 Fc Chimera (Catalog # 541-A5) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
Ephrin‑A2 in Rat Hippocampal Neurons. Ephrin‑A2 was detected in immersion fixed rat hippocampal neurons using 10 µg/mL Goat Anti-Mouse Ephrin‑A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF603) for 3 hours at room temperature. Neurons were stained (green) and glial cells were labeled by using anti-GFAP antibodies (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Ephrin-A2

Ephrin-A2, also known as ELF-1, Cek7-L and LERK-6 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A2 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of human and mouse Ephrin-A2 share 93% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression.(3) It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).

  • References:
    1. Eph Nomenclature Committee [letter] (1997) Cell 9:403.
    2. Flanagan, J.G. and P. Vanderhaegen (1998) Annu. Rev. Neurosci. 21:309.
    3. Pasquale, E.B. (1997) Curr. Opin. Cell. Biol. 9:608.
  • Entrez Gene IDs:
    1943 (Human); 13637 (Mouse)
  • Alternate Names:
    Cek7-L; EFNA2; ELF-1; EPH-related receptor tyrosine kinase ligand 6; EphrinA2; Ephrin-A2; EPLG6HEK7 ligand; HEK7-L; HEK7-ligand; LERK6; LERK6LERK-6; ligand of eph-related kinase 6
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. The mouse IAPE endogenous retrovirus can infect cells through any of the five GPI-anchored Ephrin A proteins.
    Authors: Dewannieux M, Vernochet C, Ribet D, Bartosch B, Cosset FL, Heidmann T
    PLoS Pathog., 2011;7(10):e1002309.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
  2. Bidirectional signaling through ephrinA2-EphA2 enhances osteoclastogenesis and suppresses osteoblastogenesis.
    Authors: Irie N, Takada Y, Watanabe Y, Matsuzaki Y, Naruse C, Asano M, Iwakura Y, Suda T, Matsuo K
    J. Biol. Chem., 2009;284(21):14637-44.
    Species: Mouse
    Sample Type: Cell Lysates
    Application: WB
  3. p75(NTR) mediates ephrin-A reverse signaling required for axon repulsion and mapping.
    Authors: Lim YS, McLaughlin T, Sung TC, Santiago A, Lee KF, O'Leary DD
    Neuron, 2008;59(5):746-58.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: IP
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