Detects mouse Ephrin-A2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant mouse (rm) Ephrin-A1, recombinant human (rh) Ephrin-A3, rmEphrin-A4, rhEphrin-A5, rmEphrin-B1, rmEphrin-B2, and rhEphrin-B3 is observed.
Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2)
Blockade of Receptor-ligand Interaction
In a functional ELISA, 1-5 µg/mL of this antibody will block 50% of the binding of 10 ng/mL of Recombinant Mouse Ephrin-A2 Fc Chimera (Catalog # 603-A2) to immobilized Recombinant Rat EphA5 Fc Chimera (Catalog # 541-A5) coated at 2 µg/mL (100 µL/well). At 50 μg/mL, this antibody will block >90% of the binding.
Please Note: Optimal dilutions should be determined by each laboratory for each application.
are available in the Technical Information section on our website.
Ephrin‑A2 in Rat Hippocampal Neurons.
Ephrin‑A2 was detected in immersion fixed rat hippocampal neurons using 10 µg/mL Goat Anti-Mouse Ephrin‑A2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF603) for 3 hours at room temperature. Neurons were stained (green) and glial cells were labeled by using anti-GFAP antibodies (red). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Ephrin-A2, also known as ELF-1, Cek7-L and LERK-6 (1), is a member of the Ephrin ligand family which binds members of the Eph receptor family. All ligands share a conserved extracellular sequence, which most likely corresponds to the receptor binding domain. This conserved sequence consists of approximately 125 amino acids (aa) and includes four invariant cysteines. The A-class ligands have a GPI anchor following the conserved sequence. Ephrin-A2 has been shown to bind EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphA8 (2, 3). The extracellular domains of human and mouse Ephrin-A2 share 93% aa identity. Only membrane-bound or Fc-clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression.(3) It has been found that nearly all receptors and ligands are expressed in developing and adult neural tissue (3). The Eph/Ephrin families also appear to play a role in angiogenesis (3).
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The data collected includes not only links to publications in PubMed,
but also provides information about sample types, species, and experimental conditions.
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