Mouse Erythropoietin/EPO Antibody (148409) [Biotin]

R&D Systems | Catalog # BAM9592

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Key Product Details

Species Reactivity

Mouse

Applications

ELISA Detection (Matched Antibody Pair)

Label

Biotin

Antibody Source

Monoclonal Rat IgG2B Clone # 148409
View all Erythropoietin/EPO Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant mouse Erythropoietin/EPO
Ala27-Arg192
Accession # Q0VED9

Specificity

Detects mouse Erythropoietin/EPO in ELISAs. In sandwich immunoassays, 100% cross-reactivity with canine, human and rat Erythropoietin/EPO is observed and no cross-reactivity with recombinant mouse Thrombopoietin is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse Erythropoietin/EPO Antibody (148409) [Biotin]

Mouse Erythropoietin ELISA Standard Curve

Mouse Erythropoietin ELISA Standard Curve

Recombinant Mouse Erythropoietin (Catalog # 959-ME) was serially diluted and captured by Rat Anti-Mouse Erythropoietin/EPO Monoclonal Antibody (Catalog # MAB9591) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse Erythropoietin/EPO Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Mouse Erythropoietin/EPO Antibody (148409) [Biotin]

Mouse Erythropoietin/EPO Sandwich Immunoassay

ELISA Detection (Matched Antibody Pair)
Recommended Concentration: 0.5-2.0 µg/mL
Use in combination with these reagents:
  • Capture Reagent: Mouse Erythropoietin/EPO Antibody (Catalog # MAB9591)
  • Standard: Recombinant Mouse Erythropoietin/EPO Protein (Catalog # 959-ME)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using BAM9592 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen and protein G Affinity-purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Erythropoietin/EPO

Erythropoietin (EPO) is a 34 kDa glycoprotein hormone in the type I cytokine family and is related to thrombopoietin (1). Its three N‑glycosylation sites, four alpha helices, and N- to C-terminal disulfide bond are conserved across species (2, 3). Glycosylation of EPO is required for biological activities in vivo (4). Mature mouse EPO shares 95% amino acid sequence identity with rat EPO and 73%‑82% with bovine, canine, equine, feline, human, ovine, and porcine EPO. EPO is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules (5). It is also produced in much lower, but functionally significant amounts by fetal hepatocytes and in adult liver and brain (6‑8). EPO promotes erythrocyte formation by preventing the apoptosis of early erythroid precursors which express the EPO receptor (EPO R) (8, 9). EPO R has also been described in brain, retina, heart, skeletal muscle, kidney, endothelial cells, and a variety of tumor cells (7, 8, 10, 11). Ligand induced dimerization of EPO R triggers JAK2-mediated signaling pathways followed by receptor/ligand endocytosis and degradation (1, 12). Rapid regulation of circulating EPO allows tight control of erythrocyte production and hemoglobin concentrations. Anemia or other causes of low tissue oxygen tension induce EPO production by stabilizing the hypoxia-induceable transcription factors HIF-1 alpha and HIF-2 alpha (1, 6). EPO additionally plays a tissue‑protective role in ischemia by blocking apoptosis and inducing angiogenesis (7, 8, 13).

References

  1. Koury, M. J. (2005) Exp. Hematol. 33:1263.
  2. Shoemaker, C.B. and L.D. Mitsock (1986) Mol. Cell. Biol 6:849.
  3. Wen, D. et al. (1993) Blood 82:1507.
  4. Tsuda E. et al. (1990) Eur. J. Biochem. 188:405.
  5. Lacombe, C. et al. (1988) J. Clin. Invest. 81:620.
  6. Eckardt, K. U. and A. Kurtz (2005) Eur. J. Clin. Invest. 35 Suppl. 3:13.
  7. Sharples, E. J. et al. (2006) Curr. Opin. Pharmacol. 6:184.
  8. Rossert, J. and K. Eckardt (2005) Nephrol. Dial. Transplant 20:1025.
  9. Koury, M.J. and M.C. Bondurant (1990) Science 248:378.
  10. Acs, G. et al. (2001) Cancer Res. 61:3561.
  11. Hardee, M.E. et al. (2006) Clin. Cancer Res. 12:332.
  12. Verdier, F. et al. (2000) J. Biol. Chem. 275:18375.
  13. Kertesz, N. et al. (2004) Dev. Biol. 276:101.

Alternate Names

ECYT5, EPO, MVCD2

Entrez Gene IDs

2056 (Human); 13856 (Mouse); 24335 (Rat)

Gene Symbol

EPO

UniProt

Q0VED9

Additional Erythropoietin/EPO Products

Product Documents for Mouse Erythropoietin/EPO Antibody (148409) [Biotin]

Certificate of Analysis

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Product Specific Notices for Mouse Erythropoietin/EPO Antibody (148409) [Biotin]

For research use only

Customer Reviews for Mouse Erythropoietin/EPO Antibody (148409) [Biotin] (1)

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  • Mouse Erythropoietin Biotinylated Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Recombinant protein
    Species: Canine
    Verified Customer | Posted 12/06/2017
    Mouse Erythropoietin/EPO Antibody (148409) [Biotin] BAM9592

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