Mouse Fc gamma  RI/CD64 Antibody

Catalog # Availability Size / Price Qty
MAB20741
MAB20741-SP

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Product Details
Citations (9)
FAQs
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Mouse Fc gamma  RI/CD64 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fc gamma  RI in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) Fc gamma  RIA, rhFc gamma  RIIB, or rhFc gamma  RIIIB is observed.
Source
Monoclonal Rat IgG2A Clone # 290322
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0-derived recombinant mouse Fc gamma RI extracellular domain
Glu25-Pro297
Accession # P26151
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
RAW 264.7 mouse monocyte/macrophage cell line
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fc gamma RI/CD64

Receptors for the Fc region of IgG (Fc gamma Rs) are members of the Ig superfamily that function in the activation or inhibition of immune responses such as degranulation, phagocytosis, ADCC (antibody-dependent cellular toxicity), cytokine release, and B cell proliferation (1‑3). The Fc gamma Rs have been divided into three classes based on close relationships in their extracellular domains; these groups are designated Fc gamma  RI (also known as CD64), Fc gamma  RII (CD32), and Fc gamma  RIII (CD16). Each group may be encoded by multiple genes and exist in different isoforms depending on species and cell type. The CD64 proteins are high affinity receptors (~10‑8‑10‑9 M) capable of binding monomeric IgG, whereas the CD16 and CD32 proteins bind IgG with lower affinities (~10‑6‑10‑7 M) only recognizing IgG aggregates surrounding multivalent antigens (1, 4). Fc gamma Rs that deliver an activating signal either have an intrinsic immunoreceptor tyrosine-based activation motif (ITAM) within their cytoplasmic domains or associate with one of the ITAM-bearing adapter subunits, Fc R gamma or zeta (3, 5). The only inhibitory member in human and mouse, Fc gamma  RIIb, has an intrinsic cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM). The coordinated functioning of activating and inhibitory receptors is necessary for successful initiation, amplification, and termination of immune responses (5). Mouse Fc gamma RI is transmembrane protein with three extracellular Ig-like domains, and it delivers an activating signal via the associated Fc R gamma accessory chain (1, 2). The high affinity recognition of IgG by Fc gamma RI permits the triggering of effector responses at low IgG concentrations typical of early immune responses (2). Fc gamma RI is expressed constitutively on monocytes and macrophages and can be induced on neutrophils and eosinophils (1, 4). Its expression is up-regulated during bacterial infections and sepsis.

References
  1. Van de Winkel, J. and P. Capes (1993) Immunol. Today 14:215.
  2. Raghaven, M. and P. Bjorkman (1996) Annu. Rev. Cell Dev. Biol. 12:181.
  3. Ravetch, J. and S. Bolland (2001) Annu. Rev. Immunol. 19:275.
  4. Takai, T. (2002) Nature Rev. Immunol. 2:580.
  5. Ravetch, J. and L. Lanier (2000) Science 290:84.
Long Name
Fc gamma Receptor I
Entrez Gene IDs
2209 (Human); 14129 (Mouse); 295279 (Rat); 102147198 (Cynomolgus Monkey)
Alternate Names
CD64 antigen; CD64; CD64a; Fc fragment of IgG, high affinity Ia, receptor (CD64); Fc gamma RI; FCG1; Fc-gamma receptor I B2; Fc-gamma RI; Fc-gamma RIA; FcgammaRIa; FCGR1; FcgRI; FcgRIA; FCRI; FcRIA; FLJ18345; high affinity Ia, receptor for (CD64); high affinity immunoglobulin gamma Fc receptor I; IgG Fc receptor I

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Citations for Mouse Fc gamma  RI/CD64 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Ligand-Blocking and Membrane-Proximal Domain Targeting Anti-OX40 Antibodies Mediate Potent T Cell-Stimulatory and Anti-Tumor Activity
    Authors: P Zhang, GH Tu, J Wei, P Santiago, LR Larrabee, S Liao-Chan, T Mistry, ML Chu, T Sai, K Lindquist, H Long, J Chaparro-R, S Salek-Arda, YA Yeung
    Cell Rep, 2019;27(11):3117-3123.e5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. C5aR activation in the absence of C5a - a new disease mechanism of Autoimmune Hemolytic Anemia in mice
    Authors: SN Syed, E Rau, M Ziegelmann, G Sogkas, B Brüne, RE Schmidt
    Eur. J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Megakaryocytes compensate for Kit insufficiency in murine arthritis
    Authors: P Cunin, LR Penke, JN Thon, PA Monach, T Jones, MH Chang, MM Chen, I Melki, S Lacroix, Y Iwakura, J Ware, MF Gurish, JE Italiano, E Boilard, PA Nigrovic
    J. Clin. Invest., 2017;127(5):1714-1724.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  4. Gliotoxin Suppresses Macrophage Immune Function by Subverting Phosphatidylinositol 3,4,5-Trisphosphate Homeostasis
    Authors: D Schlam, J Canton, M Carreño, H Kopinski, SA Freeman, S Grinstein, GD Fairn
    MBio, 2016;7(2):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Immune complexes regulate bone metabolism through FcRgamma signalling.
    Authors: Negishi-Koga T, Gober H, Sumiya E, Komatsu N, Okamoto K, Sawa S, Suematsu A, Suda T, Sato K, Takai T, Takayanagi H
    Nat Commun, 2015;6(0):6637.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. beta5 integrin is the major contributor to the alphaVintegrin-mediated blockade of HIV-1 replication.
    Authors: Ballana E, Pauls E, Clotet B, Perron-Sierra F, Tucker GC, Este JA
    J. Immunol., 2011;186(1):464-70.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Neuronal Fc-gamma receptor I mediated excitatory effects of IgG immune complex on rat dorsal root ganglion neurons.
    Authors: Qu L, Zhang P, Lamotte RH, Ma C
    Brain Behav. Immun., 2011;25(0):1399-407.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  8. Mouse and human neutrophils induce anaphylaxis.
    Authors: Jonsson F, Mancardi DA, Kita Y, Karasuyama H, Iannascoli B, Van Rooijen N, Shimizu T, Daeron M, Bruhns P
    J. Clin. Invest., 2011;121(4):1484-96.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  9. Dynamic macrophage "probing" is required for the efficient capture of phagocytic targets.
    Authors: Flannagan RS, Harrison RE, Yip CM, Jaqaman K, Grinstein S
    J. Cell Biol., 2010;191(6):1205-18.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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