Mouse Fetuin A/AHSG Antibody

Catalog # Availability Size / Price Qty
MAB15632
MAB15632-SP
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Mouse Fetuin A/AHSG Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Fetuin A/AHSG in ELISAs. In sandwich immunoassays, no cross-reactivty or interference was observed with recombinant human Fetuin A, rhTGF-beta 1, recombinant mouse (rm) BMP-4, rmMMP-2, recombinant rat MMP-2, or rmMMP-9 is observed.
Source
Monoclonal Rat IgG2B Clone # 207440
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Fetuin A/AHSG
Ala19-Ile345
Accession # P29699
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse Fetuin A/AHSG Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Fetuin A/AHSG Biotinylated Antibody (Catalog # BAM15631)

Standard: Recombinant Mouse Fetuin A/AHSG Protein, CF (Catalog # 1563-PI)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fetuin A/AHSG

Mouse Fetuin A, also known as alpha 2-Heremans-Schmid glycoprotein, is encoded by the AHSG gene. It has been also called "countertrypsin" because of its ability to inhibit trypsin (1). It is a major plasma protein and a member of the cystatin superfamily of protease inhibitors (2, 3). It is expressed by hepatocytes, the principal cell source, and by monocyte/macrophages (4). The major form of plasma Fetuin A corresponds to two disulfide bond-linked chains derived from the single chain (5). Fetuin-A has a number of functions. It is a negative acute-phase protein with normal circulating levels in adults (300‑600 μg/mL), which fall significantly (30‑50%) during injury and infection (5). It enhances entry of cationic inhibitors into macrophages (6). It inhibits both insulin receptor autophosphorylation and undesirable calcification (7, 8).

References
  1. Yamamoto, K. and H. Sinohara (1993) J. Biol. Chem. 268:17750.
  2. Kellemann, J. et al. (1989) J. Biol. Chem. 264:14121.
  3. Dziegielewska, K.M. et al. (1990) J. Biol. Chem. 265:4354.
  4. Dziegielewska, K.M. et al. (1996) Histochem. Cell Biol. 106:319.
  5. Gejyo, F. and K. Schmid (1981) Biochim. Biophys. Acta 671:78.
  6. Wang, H. et al. (1998) Proc. Natl. Acad. Sci. USA 95:14429.
  7. Mathews, S.T. et al. (2000) Mol. Cell Endocrinol. 164:87.
  8. Schäfer, C. et al. (2003) J. Clin. Invest. 112:357.
Long Name
alpha-2-HS Glycoprotein
Entrez Gene IDs
197 (Human); 11625 (Mouse); 25373 (Rat)
Alternate Names
A2HS; AHS; AHSG; alpha-2-HS-glycoprotein; alpha-2-Z-globulin; Ba-alpha-2-glycoprotein; FETUAba-alpha-2-glycoprotein; Fetuin A; fetuin-A; HSGA

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