Mouse FGF-10 Antibody

R&D Systems | Catalog # AF6224

R&D Systems

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all FGF-10 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse FGF-10
Ser62-Thr209
Accession # NP_032028

Specificity

Detects mouse and human FGF-10 in Western blots. In direct ELISAs, approximately 100% cross-reactivity with recombinant rat FGF‑10 is observed and less than 10% cross-reactivity with recombinant mouse FGF-6 and recombinant mouse FGF-7 is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse FGF-10 Antibody

Detection of Human FGF-10 antibody by Western Blot.

Detection of Human FGF‑10 by Western Blot.

Western blot shows lysates of A549 human lung carcinoma cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Mouse FGF-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6224) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for FGF-10 at approximately 20 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
FGF-10 antibody in Mouse Embryo by Immunohistochemistry (IHC-Fr).

FGF‑10 in Mouse Embryo.

FGF-10 was detected in immersion fixed frozen sections of mouse embryo (E13) using Sheep Anti-Mouse FGF-10 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6224) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Detection of FGF-10 by Western Blot

Detection of FGF-10 by Western Blot

Mesenchymal Alk3 cKO alters the production of proteins that have not previously been described. (A) GO (Gene Ontology) enrichment and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway analyses to present the functional associations of RNA-sequencing-detected DEGs in the tongue mesenchyme of E12.0 Wnt1-Cre/Alk3 cKO (Alk3 cKO) compared with Cre−/Alk3fx/fx littermates. (B) Histograms (mean±s.d.; n=3) presenting the FPKM values of the known regulators of Wnt/ beta -catenin signaling in the E12.0 tongue mesenchyme. Control, Cre−/Alk3fx/fx; Alk3 cKO, Wnt1-Cre/Alk3 cKO. No statistically significant differences were found with (adjusted P-value) DESeq2 statistical analysis based on read counts. (C) GO enrichment and pathway analyses to show the functional association of differentially expressed proteins in the tongue mesenchyme-conditioned medium from Wnt1-Cre/Alk3 cKO (Alk3 cKO) and Cre−/Alk3fx/fx (control). These extracted proteins were detected with liquid chromatography-mass spectrometry (LC-MS). (D) A histogram (mean±s.d.; n=3) presenting the transcripts levels (FPKM values) of Fgf10 and Fst (follistatin) in the tongue mesenchyme of Cre−/Alk3fx/fx (control) and Wnt1-Cre/Alk3 cKO (Alk3 cKO) mice. (E) Western blot bands of FGF10, Fst and Gapdh in the mesenchyme-conditioned medium (Mes-CM) or mesenchyme (Mes) tissue from E12.0 Cre−/Alk3fx/fx (Ctrl) and Wnt1-Cre/Alk3 cKO (cKO). The histogram on the right (mean±s.d.; n=3) presents the normalized band intensities of FGF10 and Fst relative to Gapdh in E12.0 Cre−/Alk3fx/fx (control) and Wnt1-Cre/Alk3 cKO (Alk3 cKO) tongue mesenchyme. No statistically significant differences were found in Alk3 cKO compared with the Cre−/Alk3fx/fx littermate controls using an unpaired Student's t-test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37680190), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Mouse FGF-10 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed frozen sections of mouse embryo (E13)

Western Blot

1 µg/mL
Sample: A549 human lung carcinoma cell line

Reviewed Applications

Read 1 review rated 4 using AF6224 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FGF-10

The Fibroblast Growth Factors (FGFs) are heparin binding glycoproteins that exert a variety of biological activities toward cells of mesenchymal, neuronal, and epithelial origin. FGF-10 belongs to the subgroup of FGFs that also includes FGF-3, -7, and -22 (1). Mature mouse FGF-10 is an approximately 20 kDa protein that contains a serine-rich region near its N-terminus (2, 3). C-terminal to this region (aa 62‑209), it shares  94% and 100% amino acid sequence identity with human and rat FGF-10, respectively. FGF-10 is secreted by mesenchymal cells and associates with extracellular FGF-BP (1, 4). It preferentially binds and activates epithelial cell FGF R2 (IIIb) and interacts more weakly with FGF R1 (IIIb) (5). The mitogenic and chemotactic properties of FGF-10 are critical in many tissues during embryogenesis. This includes limb bud initiation (6), palate development (7), branching morphogenesis and directional outgrowth of lung buds (2, 8), formation of the otic vesicle and chochlea (9), adipogenesis (10), and the development of prostate, mammary, lacrimal, and submandibular salivary glands (11‑14). FGF R2 (IIIb) signaling in these responsive tissues is similarly important during embryogenesis (7, 9, 12‑14). The expression and function of FGF‑10 are negatively regulated by Shh and BMP-4 in the developing lung (2, 8). Overlapping expression patterns and activities with FGF-3, -7,  and -8 suggest at least a partial redundancy in FGF-10 biology (7, 9, 13, 14). FGF-10 induced signaling through FGF R2 (IIIb) also contributes to the progression of pancreatic cancer (15).

References

  1. Beenken, A. and M. Mohammadi (2009) Nat. Rev. Drug Discov. 8:235.
  2. Bellusci, S. et al. (1997) Development 124:4867.
  3. Tagashira, S. et al. (1997) Gene 197:399.
  4. Beer, H.-D. et al. (2005) Oncogene 24:5269.
  5. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
  6. Min, H. et al. (1998) Genes Dev. 12:3156.
  7. Rice, R. et al. (2004) J. Clin. Invest. 113:1692.
  8. Weaver, M. et al. (2000) Development 127:2695.
  9. Pirvola, U. et al. (2000) J. Neurosci. 20:6125.
  10. Sakaue, H. et al. (2002) Genes Dev. 16:908.
  11. Donjacour, A.A. et al. (2003) Dev. Biol. 261:39.
  12. Mailleux, A.A. et al. (2002) Development 129:53.
  13. Makarenkova, H.P. et al. (2000) Development 127:2563.
  14. Jaskoll, T. et al. (2005) BMC Dev. Biol. 5:11.
  15. Nomura, S. et al. (2008) Br. J. Cancer 99:305.

Long Name

Fibroblast Growth Factor 10

Alternate Names

FGF10, KGF-2, KGF2

Entrez Gene IDs

2255 (Human); 14165 (Mouse); 25443 (Rat)

Gene Symbol

FGF10

UniProt

NP_032028

Additional FGF-10 Products

Product Documents for Mouse FGF-10 Antibody

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Product Specific Notices for Mouse FGF-10 Antibody

For research use only

Related Research Areas

FGF Family
FGF Ligands
gamma delta T Cells

Citations for Mouse FGF-10 Antibody

Customer Reviews for Mouse FGF-10 Antibody (1)

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  • Mouse FGF-10 Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: E9.5 mouse embryo fixed in 4% PFA
    Species: Mouse
    Verified Customer | Posted 12/02/2020
    Antibody was used on E9.5 mouse sections. Concentration used 10ug/mL.
    Mouse FGF-10 Antibody AF6224

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