hh Mouse FGF-23 Antibody MAB26291: R&D Systems

Mouse FGF-23 Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse FGF-23 in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 50% cross-reactivity with recombinant human (rh) FGF-23. In Western blots, this antibody does not cross-react with rhFGF-3, -4, -5, -7, -9, -10, -11, -12, -13, -16, -17, -18, -19, acidic, basic, rmFGF-8b, -8c, -15, or -21.
  • Source
    Monoclonal Rat IgG2A Clone # 283507
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse FGF-23
    Tyr25-Val251 (Arg179Gln)
    Accession # Q9EPC2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Immunohistochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
FGF‑23 in Mouse Brain. FGF‑23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF‑23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-23

Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3-6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGF R:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGF R1 and 3 plus FGF R4, Klotho has a restricted distribution that limits FGF-23 activity (10-12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14-16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).

  • References:
    1. Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563. 
    2. Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
    3. Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
    4. Huang, X. et al. (2006) Mol. Carcinog. 45:934. 
    5. Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
    6. Harmer, N.J. et al. (2004) Biochemistry 43:629.
    7. Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
    8. Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
    9. Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
    10. Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
    11. Urakawa, I. et al. (2006) Nature 444:770.
    12. Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
    13. Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
    14. Moore, D.D. (2007) Science 316:1436.
    15. Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
    16. Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
    17. Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
    18. Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.
  • Long Name:
    Fibroblast Growth Factor 23
  • Entrez Gene IDs:
    8074 (Human); 64654 (Mouse)
  • Alternate Names:
    ADHR; FGF23; FGF-23; fibroblast growth factor 23; HPDR2; HYPF; phosphatonin; PHPTC; tumor-derived hypophosphatemia inducing factor; Tumor-derived hypophosphatemia-inducing factor
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
    Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
    Biomed. Res., 2017;38(4):257-267.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC paraffin embedded
  2. Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs
    Authors: Z Liu, T Yamamoto, T Hasegawa, H Hongo, K Tsuboi, E Tsuchiya, M Haraguchi, M Abe, PH Freitas, A Kudo, K Oda, M Li, N Amizuka
    Biomed Res, 2016;37(2):141-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Paraffin-embedded
  3. Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
    Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
    Kidney Int, 2014;85(6):1340-50.
    Species: Rat
    Sample Type: Whole Tissue
    Application: IHC Frozen
  4. Renal phosphate wasting due to tumor-induced osteomalacia: a frequently delayed diagnosis.
    Authors: Gore MO, Welch BJ, Geng W, Kabbani W, Maalouf NM, Zerwekh JE, Moe OW, Sakhaee K
    Kidney Int., 2008;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: ICC
Cell and Tissue Staining Kits
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Anti-Rat HRP-DAB Cell & Tissue Staining Kit

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Isotype Controls
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Normal Rat IgG Control

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Rat IgG2A Isotype Control

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Secondary Antibodies
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Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

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Goat Anti-Rat IgG Biotinylated Antibody

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Rat IgG VisUCyte HRP Polymer Antibody

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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

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