Mouse FGF-23 Antibody
Mouse FGF-23 Antibody Summary
Tyr25-Val251 (Arg179Gln)
Accession # Q9EPC2
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Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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FGF‑23 in Mouse Brain. FGF-23 was detected in perfusion fixed frozen sections of mouse brain (cortex) using Rat Anti-Mouse FGF-23 Monoclonal Antibody (Catalog # MAB26291) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific staining was localized to glial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: FGF-23
Fibroblast growth factor 23 (FGF-23) is a 30-32 kDa member of the FGF gene family. Based on its structure, it is further classified as an FGF19 subfamily member. This subfamily includes FGF-19, -21, and -23. Like all other FGF subfamilies, FGF-19 subfamily members contain a 120 amino acid (aa) core FGF domain that exhibits a beta -trefoil structure (1, 2). Unlike other FGF subfamilies, FGF-19 subfamily members exist as highly diffusible molecules that is attributed to poor ECM/heparin sulfate binding (3-6). The cDNA for mouse FGF-23 predicts a 251 aa polypeptide that contains a 24 aa signal sequence and a 227 aa mature region (7). Mature mouse FGF-23 shows 72% aa identity to human FGF-23 (8). The FGF-19 subfamily shares an unusual receptor configuration. The standard model for FGF signaling requires an FGF:FGF R:heparin sulfate complex. Given FGF-23’s minimal association with heparin, a substitute termed ( alpha -) Klotho has evolved that serves the same function. Although FGF-23 binds to the widely expressed “c” isoforms of FGF R1 and 3 plus FGF R4, Klotho has a restricted distribution that limits FGF-23 activity (10-12). It should be noted that heparin-dependency has been reported for FGF-19 signaling, and this observation may extend to FGF-23 (13). The FGF-19 subfamily is considered endocrine in nature. All three subfamily members impact some aspect of metabolism and all three are induced by a nuclear receptor heterodimer that includes the retinoid X receptor (14-16). FGF-23 is considered a phosphatonin; that is, a molecule that reduces circulating plasma phosphate. It is produced by osteocytes and osteoblasts in response to high circulating phosphate levels, elevated parathyroid hormone that induces hypercalcemia, and circulatory volume loading. Upon binding to FGF-23 receptors on renal proximal tubular epithelium, two basic changes are seen. First, the enzyme responsible for generating the active form of vitamin D is suppressed, resulting in decreased levels of bioactive vitamin D. Since vitamin D promotes intestinal phosphate absorption, plasma phosphate declines. Second, the transporters responsible for phosphate resorption on renal epithelium are down regulated, resulting in decreased uptake from urine and again a decline in blood phosphorus (17, 18).
- Itoh, N. and D.M. Ornitz (2004) Trends Genet. 20:563.
- Mohammadi, M. et al. (2005) Cytokine Growth Factor Rev. 16:107.
- Fukumoto, S. (2007) Endocr. J. Sep 14; [Epub ahead of print].
- Huang, X. et al. (2006) Mol. Carcinog. 45:934.
- Goetz, R. et al. (2007) Mol. Cell. Biol. 27:3417.
- Harmer, N.J. et al. (2004) Biochemistry 43:629.
- Yamashita, T. et al. (2000) Biochem. Biophys. Res. Commun. 277:494.
- Shimada, T. et al. (2001) Proc. Natl. Acad. Sci. USA 98:6500.
- Kato, K. et al. (2006) J. Biol. Chem. 281:18370.
- Zhang, X. et al. (2006) J. Biol. Chem. 281:15694.
- Urakawa, I. et al. (2006) Nature 444:770.
- Hurosu, H. et al. (2006) J. Biol. Chem. 281:6120.
- Wu, X. et al. (2007) J. Biol. Chem. 282:29069.
- Moore, D.D. (2007) Science 316:1436.
- Ogawa, Y. et al. (2007) Proc. Natl. Acad. Sci. USA 104:7432.
- Kurosu, H. et al. (2007) J. Biol. Chem. 282:26687.
- Razzaque, M.S. and B. Lanske (2007) J. Endocrinol. 194:1.
- Liu, S. et al. (2007) Curr. Opin. Nephrol. Hypertens. 16:329.
Product Datasheets
Citations for Mouse FGF-23 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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FGF receptor inhibitor BGJ398 partially rescues osteoarthritis-like phenotype in older high molecular weight FGF2 transgenic mice via multiple mechanisms
Authors: MM Hurley, JD Coffin, T Doetschman, C Valera, K Clarke, L Xiao
Scientific Reports, 2022;12(1):15968.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Induction of somatopause in adult mice compromises bone morphology and exacerbates bone loss during aging
Authors: M Dixit, S Duran-Orti, G Yildirim, SB Poudel, LD Louis, A Bartke, MB Schaffler, JJ Kopchick, S Yakar
Aging Cell, 2021;0(0):e13505.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Lack of PTEN in osteocytes increases circulating phosphate concentrations by decreasing intact fibroblast growth factor 23 levels
Authors: M Kawai, S Kinoshita, K Ozono, T Michigami
Scientific Reports, 2020;10(1):21501.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC -
Fibroblast growth factor 23 is upregulated in the kidney in a chronic kidney disease rat model
Authors: H Sugiura, A Matsushita, M Futaya, A Teraoka, KI Akiyama, N Usui, N Nagano, K Nitta, K Tsuchiya
PLoS ONE, 2018;13(3):e0191706.
Species: Rat
Sample Types: Tissue Homogenates
Applications: Western Blot -
Chronological immunolocalization of sclerostin and FGF23 in the mouse metaphyseal trabecular and cortical bone
Authors: A Sakurai, T Hasegawa, A Kudo, Z Shen, T Nagai, M Abe, T Yoshida, H Hongo, T Yamamoto, T Yamamoto, K Oda, PHL Freitas, M Li, H Sano, N Amizuka
Biomed. Res., 2017;38(4):257-267.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Targeted disruption of NF1 in osteocyte increases FGF23 and osteoid with osteomalacia-like bone phenotype
Authors: N Kamiya, R Yamaguchi, O Aruwajoye, A Kim, G Kuroyanagi, M Phipps, NS Adapala, JQ Feng, HKW Kim
J. Bone Miner. Res., 2017;0(0):.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Dietary phosphate supplement does not rescue skeletal phenotype in a mouse model for craniometaphyseal dysplasia
Authors: I-Ping Chen
J Negat Results Biomed, 2016;15(1):18.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Immunolocalization of osteocyte-derived molecules during bone fracture healing of mouse ribs
Authors: Z Liu, T Yamamoto, T Hasegawa, H Hongo, K Tsuboi, E Tsuchiya, M Haraguchi, M Abe, PH Freitas, A Kudo, K Oda, M Li, N Amizuka
Biomed Res, 2016;37(2):141-51.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Renal expression of FGF23 and peripheral resistance to elevated FGF23 in rodent models of polycystic kidney disease.
Authors: Spichtig D, Zhang H, Mohebbi N, Pavik I, Petzold K, Stange G, Saleh L, Edenhofer I, Segerer S, Biber J, Jaeger P, Serra A, Wagner C
Kidney Int, 2014;85(6):1340-50.
Species: Rat
Sample Types: Whole Tissue
Applications: IHC-Fr -
Renal phosphate wasting due to tumor-induced osteomalacia: a frequently delayed diagnosis.
Authors: Gore MO, Welch BJ, Geng W, Kabbani W, Maalouf NM, Zerwekh JE, Moe OW, Sakhaee K
Kidney Int., 2008;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
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