Mouse G-CSFR/CD114 Antibody

Catalog # Availability Size / Price Qty
MAB6039
MAB6039-SP
Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF R/CD114 Antibody.
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Product Details
Citations (2)
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Mouse G-CSFR/CD114 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse G-CSF R/CD114 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) G-CSF R, rhGM‑CSF R beta, recombinant mouse (rm) GM-CSF R alpha, or rmM-CSF R is observed.
Source
Monoclonal Rat IgG2B Clone # 680206
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse G-CSF R/CD114
Cys26-Asp626
Accession # P40223
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.01 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Neutralization
Measured by its ability to neutralize G‑CSF-induced proliferation in the M‑NFS‑60 mouse myelogenous leukemia lymphoblast cell line. Shirafuji, N. et al. (1989) Exp. Hematol. 17:116. The Neutralization Dose (ND50) is typically 0.015-0.075 µg/mL in the presence of 0.15 ng/mL Recombinant Mouse G‑CSF.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Neutralization Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF R/CD114 Antibody. View Larger

Cell Proliferation Induced by G‑CSF and Neutralization by Mouse G‑CSF R/CD114 Antibody. Recombinant Mouse G-CSF (Catalog # 414-CS) stimulates proliferation in the M-NFS-60 mouse myelogenous leukemia lymphoblast cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse G-CSF (0.15 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse G-CSF R/CD114 Monoclonal Antibody (Catalog # MAB6039). The ND50 is typically 0.015-0.075 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: G-CSFR/CD114

Granulocyte colony stimulating factor (G-CSF) is a pleiotropic cytokine best known for its specific effects on the proliferation, differentiation, and activation of hematopoietic cells of the neutrophilic and granulocyte lineage (1). G-CSF plays an important role in defense against infection, in inflammation and repair, and in the maintenance of steady state hematopoiesis. Cell activation by G‑CSF is mediated by granulocyte colony stimulating factor receptor alpha (G-CSF R; also CD114), a 95‑105 kDa type I transmembrane protein and member of the cytokine receptor superfamily, type I cytokine receptor family, and type 2 subfamily of receptor proteins. Mouse G‑CSF R is synthesized as an 837 amino acid (aa) precursor that contains a 25 aa signal sequence, a 601 aa extracellular domain (ECD), a 24 aa transmembrane region, and a 187 aa cytoplasmic tail. The ECD contains one Ig-like C2-type domain, five fibronectin type-III domains, and 11 potential sites for N‑linked glycosylation. Within the ECD there is also a WSXWS motif (aa 319‑323) that is necessary for proper protein folding and thereby efficient intracellular transport and cell-surface receptor binding (2). Also, within the cytoplasmic domain there is a Box 1 motif which is required for JAK interaction and/or activation (1). Mouse G‑CSF R shares 63% aa sequence identity with human G‑CSF R. G-CSF R is expressed in mature neutrophils, neutrophilic precursors, myeloid leukemia cells, and placenta (1). Mutations have been found in the gene encoding G-CSF R in some patients with severe congenital neutropenia (1). These mutations typically lead to a truncation in the cytoplasmic domain of the G-CSF R leading to maturation arrest of neutrophilic precursors in the bone marrow and neutropenia in peripheral blood (3). Binding of G-CSF to its receptor induces dimerization or oligomerization of the receptor activating cytoplasmic tyrosine kinases (2). Signal transduction from pathways that involve Janus tyrosine kinases/signal transducer and activator of transcription proteins (Jak1, Jak2, and Tyk2/STAT3 and STATG), src-related protein tyrosine kinases (Lyn and Syk), Ras/MAP kinase, and phosphatidylinositol have been reported to be activated upon G-CSF stimulation (4).

References
  1. Ward, A.C. (2007) Front. Biosci. 12:608.   
  2. Layton, J.E. and N.E. Hall (2006) Front. Biosci. 11:3181.
  3. Mitsui, T. et al. (2003) Blood 101:2990.
  4. Nicola, N.A. in Cytokine Reference, 2001, Oppenheim, J.J. and M. Feldmann, eds. Academic Press p.1935.
 
Long Name
Granulocyte Colony Stimulating Factor Receptor
Entrez Gene IDs
1441 (Human); 12986 (Mouse)
Alternate Names
CD114 antigen; CD114; colony stimulating factor 3 receptor (granulocyte); CSF3R; Csfgr; G-CSF R; G-CSF receptor; GCSFR; G-CSFR; GCSFRG-CSF-R; granulocyte colony-stimulating factor receptor

Product Datasheets

Citations for Mouse G-CSFR/CD114 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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  1. Granulocyte Colony-Stimulating Factor Does Not Influence Clostridium Perfringens α-Toxin-Induced Myonecrosis in Mice
    Authors: M Takehara, Y Sonobe, H Bandou, K Kobayashi, M Nagahama
    Toxins (Basel), 2019;11(9):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization
  2. LECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells
    Nat Commun, 2016;7(0):12719.
    Species: Mouse
    Sample Types: In Vivo
    Applications: Neutralization

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