Mouse Galectin-7 Antibody

Catalog # Availability Size / Price Qty
MAB1304
MAB1304-SP
Galectin‑7 in Mouse Thymus.
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Mouse Galectin-7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Galectin-7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Galectin‑1, ‑3, ‑4, recombinant human (rh) Galectin-2, -7, or -8 is observed. In Western blots, approximately 20% cross-reactivity with rhGalectin‑3, approximately 10% cross-reactivity with rhGalectin-7, and no cross-reactivity with rhGalectin-1 is observed.
Source
Monoclonal Rat IgG2A Clone # 212923
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant mouse Galectin‑7
Ser2-Phe136
Accession # AAK29385
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse Galectin‑7 (Catalog # 1304-GA)
Immunohistochemistry
8-25 µg/mL
See below

Mouse Galectin-7 Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse Galectin‑7 Biotinylated Antibody (Catalog # BAF1304)

Standard: Recombinant Mouse Galectin-7 Protein (Catalog # 1304-GA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry Galectin-7 antibody in Mouse Thymus by Immunohistochemistry (IHC-Fr). View Larger

Galectin‑7 in Mouse Thymus. Galectin-7 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse Galectin-7 Monoclonal Antibody (Catalog # MAB1304) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Mouse Galectin-7 is a prototype monomeric galectin. It is expressed in stratified epithelia and is significantly down-regulated in squamous cell carcinomas. Galectin-7 is a pro-apoptotic protein that is highly induced by the tumor suppressor protein p53. It functions intracellularly upstream of JNK activation to enhance cytochrome c release during apoptosis (5). Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re‑epithelialization of wounds (6).

References
  1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
  2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
  3. Hughes, R.C. (2002) Biochimie 83:667.
  4. R&D Systems' Cytokine Bulletin, Summer (2002).
  5. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
  6. Cao, Z. et al. (2002) J. Biol. Chem. 277:42299.
Entrez Gene IDs
3963 (Human); 16858 (Mouse)
Alternate Names
GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1

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Mouse Galectin‑7 Antibody
By Anonymous on 10/26/2015
Application: IHC Sample Tested: Human tissue Species: Human

This version of antibody is very specific and works well at 1:500 dilution and is almost equal to polyclonal version.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:500