Mouse Galectin-7 Antibody

(1 Review)
  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Galectin-7 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse Galectin‑1, ‑3, ‑4, recombinant human (rh) Galectin-2, -7, or -8 is observed. In Western blots, approximately 20% cross-reactivity with rhGalectin‑3, approximately 10% cross-reactivity with rhGalectin-7, and no cross-reactivity with rhGalectin-1 is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 212923
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Galectin‑7
    Ser2-Phe136
    Accession # AAK29385
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    Recombinant Mouse Galectin‑7 (Catalog # 1304-GA)
  • Immunohistochemistry
    8-25 µg/mL
    See below
    • Mouse Galectin-7 Sandwich Immunoassay
      Reagent
  • ELISA Capture (Matched Antibody Pair)
    2-8 µg/mL 
    Mouse Galectin‑7 Antibody (Catalog # MAB1304)
  • ELISA Detection (Matched Antibody Pair)
    0.1-0.4 µg/mL 
    Mouse Galectin‑7 Biotinylated Antibody (Catalog # BAF1304)
  • ELISA Standard
     
    Recombinant Mouse Galectin-7 Protein (Catalog # 1304-GA)
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Galectin‑7 in Mouse Thymus.

Galectin‑7 was detected in perfusion fixed frozen sections of mouse thymus using Rat Anti-Mouse Galectin‑7 Monoclonal Antibody (Catalog # MAB1304) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of lymphocytes. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Galectin-7

The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified non-classical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell-surface glycoproteins (1‑4).

Mouse Galectin-7 is a prototype monomeric galectin. It is expressed in stratified epithelia and is significantly down-regulated in squamous cell carcinomas. Galectin-7 is a pro-apoptotic protein that is highly induced by the tumor suppressor protein p53. It functions intracellularly upstream of JNK activation to enhance cytochrome c release during apoptosis (5). Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re‑epithelialization of wounds (6).

  • References:
    1. Rabinovich, A. et al. (2002) TRENDS in Immunol. 23:313.
    2. Rabinovich, A. et al. (2002) J. Leukocyte Biology 71:741.
    3. Hughes, R.C. (2002) Biochimie 83:667.
    4. R&D Systems' Cytokine Bulletin, Summer (2002).
    5. Kuwabara, I. et al. (2002) J. Biol. Chem. 277:3487.
    6. Cao, Z. et al. (2002) J. Biol. Chem. 277:42299.
  • Entrez Gene IDs:
    3963 (Human); 16858 (Mouse)
  • Alternate Names:
    GAL7; Gal-7; galectin 7; Galectin7; Galectin-7; HKL-14; lectin, galactoside-binding, soluble, 7; LGALS7; LGALS7A; p53-induced gene 1 protein; PI7; PIG1; TP53I1
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Average Rating: 5 (Based on 1 review)

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We have 1 review tested in 1 species: Human.
We have 1 review tested in 1 application: Immunohistochemistry.

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Images Ratings Applications Species Reviewed By Date Details
Immunohistochemistry Mouse Galectin‑7 Antibody MAB1304
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Excellent
 IHC Human Anonymous 10/26/2015
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Immunohistochemistry Mouse Galectin‑7 Antibody MAB1304
Immunohistochemistry: Mouse Galectin‑7 Antibody [MAB1304]

Summary

ApplicationImmunohistochemistry
Sample TestedHuman tissue
SpeciesHuman

Other Experimental Details

Other Experimental DetailsThis version of antibody is very specific and works well at 1:500 dilution and is almost equal to polyclonal version.
Specificity: Specific
Sensitivity: Sensitive
Buffer: PBS
Dilution: 1:500

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