Mouse GDF-7/BMP-12 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF779
Product Details
FAQs
Supplemental Products
Reviews

Mouse GDF-7/BMP-12 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF‑7/BMP‑12 in Western blots. In Western blots, approximately 10% cross‑reactivity with recombinant mouse (rm) GDF-5 and rmGDF-6  is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF-8, rmGDF-9, and recombinant human GDF-11 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF‑7/BMP‑12
Thr316-Arg461
Accession # P43029
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GDF‑7/BMP‑12 (Catalog # 779-G7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-7/BMP-12

Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. Mature GDF-7 contains 146 aa residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80‑86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.

References
  1. Storm, E.E., et al. (1994) Nature 368:639.
  2. Nishitoh, H., et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H., et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
Long Name
Growth Differentiation Factor 7
Entrez Gene IDs
151449 (Human); 238057 (Mouse)
Alternate Names
BMP12; BMP-12; GDF7; GDF-7; growth differentiation factor 7; growth/differentiation factor 7

Product Datasheets

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse GDF-7/BMP-12 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse GDF-7/BMP-12 Biotinylated Antibody and earn rewards!

Have you used Mouse GDF-7/BMP-12 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review