Mouse GDF-7/BMP-12 Antibody

Catalog # Availability Size / Price Qty
AF779
AF779-SP
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Mouse GDF-7/BMP-12 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GDF-7 in direct ELISAs and Western blots. In these formats, approximately 10% cross‑reactivity with recombinant mouse (rm) GDF-5 and rmGDF-6 is observed and less than 1% cross-reactivity with rmGDF-1, rmGDF-3, rmGDF‑8, rmGDF-9, and recombinant human GDF-11 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse GDF-7 (R&D Systems, Catalog # 779-G7)
Thr316-Arg461
Accession # P43029
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GDF‑7/BMP‑12 (Catalog # 779-G7)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GDF-7/BMP-12

Growth Differentiation Factor 7 (GDF-7), also known as bone morphogenetic protein (BMP) 12 or cartilage derived morphogenetic protein 3 (CDMP-3), is a member of the bone morphogenetic protein (BMP) family which belongs to the transforming growth factor  beta (TGF-beta ) superfamily. Mature GDF-7 contains 146 aa residues with the characteristic seven conserved cysteine residues. The bioactive GDF-7 is a homodimer with an apparent molecular weight of approximately 28 kDa. Mature GDF-5, GDF-6 and GDF-7, which share 80 - 86% identity with each other, define a new subgroup within the BMP family. Like other TGF-beta superfamily proteins, GDF-7 is highly conserved across species. At the amino acid sequence level, mature human and mouse GDF-7 are 98% identical. It has been reported that GDF-7 has multiple functions including regulation of chondrogenesis, bone morphogenesis, and neuron differentiation. GDF-7 response is mediated by the formation of hetero-oligomeric complexes of type I (BMPR-IB) and type II (BMPR-II or Activin R-II) sereine/threonine kinase receptors, and the activiation of Smad proteins.

References
  1. Storm, E.E. et al. (1994) Nature 368:639.
  2. Nishitoh, H. et al. (1996) J. Biol. Chem. 271:21345.
  3. Francis-West, P.H. et al. (1999) Development 126:1035.
  4. Massague, J. et al. (2000) Genes and Dev. 14:627.
Long Name
Growth Differentiation Factor 7
Entrez Gene IDs
151449 (Human); 238057 (Mouse)
Alternate Names
BMP12; BMP-12; GDF7; GDF-7; growth differentiation factor 7; growth/differentiation factor 7

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