Mouse GFR alpha -3/GDNF R alpha -3 Antibody

Catalog # Availability Size / Price Qty
AF2645
AF2645-SP
GFR alpha ‑3/GDNF R alpha ‑3 in Mouse Embryo.
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Product Details
Citations (5)
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Mouse GFR alpha -3/GDNF R alpha -3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse GFR alpha -3/GDNF R alpha -3 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human GFR alpha -3 is observed and less than 2% cross-reactivity with recombinant mouse (rm) GFR alpha -2, rmGFR alpha -4, and recombinant rat GFR alpha -1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant mouse GFR alpha -3/GDNF R alpha -3
Glu34-Arg379
Accession # AAB70931
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse GFR alpha ‑3/GDNF R alpha ‑3 Fc Chimera (Catalog # 2645-FR)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunohistochemistry GFR alpha ‑3/GDNF R alpha ‑3 in Mouse Embryo. View Larger

GFR alpha ‑3/GDNF R alpha ‑3 in Mouse Embryo. GFR alpha ‑3/GDNF R alpha ‑3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse GFR alpha ‑3/GDNF R alpha ‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to developing dorsal root ganglion and spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: GFR alpha-3/GDNF R alpha-3

Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3) is a member of the GDNF family of receptors. It is one of four known members, all of which contain three conserved cysteine repeats (1, 3, 4). It is synthesized as a 397 aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa mature segment, and a propeptide segment of 26 aa that is removed in the mature protein. The mature globular membrane glycoprotein has an approximate molecular weight of 50 kDa (5), contains three potential N-linked glycosylation sites, and a hydrophobic stretch of residues at its COOH terminus that comprises a GPI linkage sequence (2, 3, 5). Mouse GFR alpha -3 shares 81% aa sequence identity with human GFR alpha -3. High-level expression of GFR alpha -3 is observed only during early stages of neurogenesis in the central nervous system and in developing and adult peripheral nerves, organs, and ganglia (2, 3, 5, 7). The expression and proportions of GFR alpha -3 are closely linked to those of the Ret receptor tyrosine kinase, particularly in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (3). GFR alpha -3 associates with Ret to form the receptor complex for the GDNF family ligand artemin, which, in turn, activates the complex (1, 6, 7). The activated complex’s signal is required for the development and maintenance of superior cervical ganglion (SCG), specifically the rostral migration of SCG precursors between embryonic days 11.5 and 14.5, and the survival of SCG neurons after birth (8).

References
  1. Xinquan, W. et al. (2006) Structure 14:1083.
  2. Worby, C. et al. (1998) J. Biol. Chem. 273:3502.
  3. Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
  4. Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
  5. Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
  6. Saarma, M. (2000) Eur. J. Biochem. 267:6968.
  7. Baloh, R. et al. (1998) Neuron 21:1291.
  8. Nishino, J et al. (1999) Neuron 23:725.
Long Name
Glial Cell line-derived Neurotrophic Factor Receptor alpha 3
Entrez Gene IDs
2676 (Human); 14587 (Mouse)
Alternate Names
GDNF family receptor alpha 3; GDNF family receptor alpha-3; GDNF R alpha-3; GDNF receptor alpha-3; GDNFR-alpha-3; GFR alpha3; GFR alpha-3; GFRA3; GFRa-3; GFR-alpha-3; glial cell line-derived neurotrophic factor receptor alpha-3; GPI-linked receptor

Product Datasheets

Citations for Mouse GFR alpha -3/GDNF R alpha -3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
    Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
    Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493.
    Species: Mouse
    Sample Types:
    Applications: WB
  2. Artemin growth factor increases nicotinic cholinergic receptor subunit expression and activity in nociceptive sensory neurons.
    Authors: Albers K, Zhang X, Diges C, Schwartz E, Yang C, Davis B, Gold M
    Mol Pain, 2014;10(0):31.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC
  3. Vascular-derived artemin: a determinant of vascular sympathetic innervation?
    Authors: Damon DH, Teriele JA, Marko SB
    Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H266-73.
    Species: Rat
    Sample Types: Whole Cells
    Applications: ICC
  4. Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
    Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
    J. Neurosci., 2006;26(33):8588-99.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold.
    Authors: Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM
    J. Neurosci., 2006;26(33):8578-87.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC

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