Mouse GFR alpha -3/GDNF R alpha -3 Antibody Summary
Accession # AAB70931
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
GFR alpha ‑3/GDNF R alpha ‑3 in Mouse Embryo. GFRa‑3/GDNF Ra‑3 was detected in immersion fixed frozen sections of mouse embryo (15 d.p.c.) using 15 µg/mL Goat Anti-Mouse GFRa‑3/GDNF Ra‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2645) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to developing dorsal root ganglion and spinal cord. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: GFR alpha-3/GDNF R alpha-3
Mouse glial cell line-derived neurotrophic factor (GDNF) family receptor alpha 3 (GFR alpha -3) is a member of the GDNF family of receptors. It is one of four known members, all of which contain three conserved cysteine repeats (1, 3, 4). It is synthesized as a 397 aa precursor, with a hydrophobic signal sequence of 28 aa, a 343 aa mature segment, and a propeptide segment of 26 aa that is removed in the mature protein. The mature globular membrane glycoprotein has an approximate molecular weight of 50 kDa (5), contains three potential N-linked glycosylation sites, and a hydrophobic stretch of residues at its COOH terminus that comprises a GPI linkage sequence (2, 3, 5). Mouse GFR alpha -3 shares 81% aa sequence identity with human GFR alpha -3. High-level expression of GFR alpha -3 is observed only during early stages of neurogenesis in the central nervous system and in developing and adult peripheral nerves, organs, and ganglia (2, 3, 5, 7). The expression and proportions of GFR alpha -3 are closely linked to those of the Ret receptor tyrosine kinase, particularly in the trigeminal ganglion, pituitary gland, thymus, lung, and duodenum (3). GFR alpha -3 associates with Ret to form the receptor complex for the GDNF family ligand artemin, which, in turn, activates the complex (1, 6, 7). The activated complex’s signal is required for the development and maintenance of superior cervical ganglion (SCG), specifically the rostral migration of SCG precursors between embryonic days 11.5 and 14.5, and the survival of SCG neurons after birth (8).
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- Naveilhan, P. et al. (1998) Proc. Natl. Acad. Sci. USA 95:1295.
- Nomoto, S. et al. (1998) Biochem. Biophys. Res. Commun. 244:849.
- Baloh, R. et al. (1998) Proc. Natl. Acad. Sci. USA 95:5801.
- Saarma, M. (2000) Eur. J. Biochem. 267:6968.
- Baloh, R. et al. (1998) Neuron 21:1291.
- Nishino, J et al. (1999) Neuron 23:725.
Citations for Mouse GFR alpha -3/GDNF R alpha -3 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 5
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Novel combinatorial screening identifies neurotrophic factors for selective classes of motor neurons
Authors: S Schaller, D Buttigieg, A Alory, A Jacquier, M Barad, M Merchant, D Gentien, P de la Gran, G Haase
Proc. Natl. Acad. Sci. U.S.A, 2017;114(12):E2486-E2493.
Applications: Western Blot
Artemin growth factor increases nicotinic cholinergic receptor subunit expression and activity in nociceptive sensory neurons.
Authors: Albers K, Zhang X, Diges C, Schwartz E, Yang C, Davis B, Gold M
Mol Pain, 2014;10(0):31.
Sample Types: Whole Tissue
Vascular-derived artemin: a determinant of vascular sympathetic innervation?
Authors: Damon DH, Teriele JA, Marko SB
Am. J. Physiol. Heart Circ. Physiol., 2007;293(1):H266-73.
Sample Types: Whole Cells
Glial cell line-derived neurotrophic factor family members sensitize nociceptors in vitro and produce thermal hyperalgesia in vivo.
Authors: Malin SA, Molliver DC, Koerber HR, Cornuet P, Frye R, Albers KM, Davis BM
J. Neurosci., 2006;26(33):8588-99.
Sample Types: Whole Cells
Artemin overexpression in skin enhances expression of TRPV1 and TRPA1 in cutaneous sensory neurons and leads to behavioral sensitivity to heat and cold.
Authors: Elitt CM, McIlwrath SL, Lawson JJ, Malin SA, Molliver DC, Cornuet PK, Koerber HR, Davis BM, Albers KM
J. Neurosci., 2006;26(33):8578-87.
Sample Types: Whole Tissue
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