Mouse gp130 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB4681A-100UG
FAB4681A-025UG
Detection of gp130 in Mouse Splenocytes by Flow Cytometry.
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Product Details
Citations (6)
FAQs
Supplemental Products
Reviews (1)

Mouse gp130 APC-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse gp130 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human gp130 is observed.
Source
Monoclonal Rat IgG2A Clone # 125623
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse gp130
Gln23-Glu617 (predicted)
Accession # Q6PDI9
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of gp130 antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of gp130 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 PE-conjugated Monoclonal Antibody (Catalog # FAB4841P) and either (A) Rat Anti-Mouse gp130 APC-conjugated Monoclonal Antibody (Catalog # FAB4681A) or (B) Rat IgG2AAllophycocyanin Isotype Control (Catalog # IC006A). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: gp130

Gp130, the common signal transducing receptor component shared by the functional receptor complexes of the IL-6 family of cytokines, belongs to the class I cytokine receptor family. Binding of IL-6 (IL-11) to either the membrane-anchored or soluble IL-6 R (IL-11 R) initiates the association of IL-6 R (IL-11 R) with gp130 which then undergoes homo-dimerization and signal transduction. With other IL-6 family cytokines, such as LIF and OSM, signal transduction is triggered by the hetero-dimerization of gp130 and LIF R or OSM R.

Gp130 is expressed in all organs examined. Soluble gp130, which apparently arises either from proteolytic cleavage of the membrane-bound receptor or from alternative splicing, has been detected in human serum. The in vivo functions of soluble gp130 are not clearly understood. In in vitro experiments, natural or recombinant soluble gp130 has been shown to have inhibitory effects on OSM and CNTF activities.

References
  1. Narazaki, M. et al. (1993) Blood 82:1120.
  2. Taga, T. and T. Kishimoto (1997) Annu. Rev. Immunol. 15:797.
Long Name
Glycoprotein 130
Entrez Gene IDs
3572 (Human); 16195 (Mouse); 25205 (Rat); 101926138 (Cynomolgus Monkey)
Alternate Names
CD130 antigen; CD130; CDw130; DKFZp564F053; gp130 of the rheumatoid arthritis antigenic peptide-bearing soluble form; gp130; IL-6 receptor subunit beta; IL-6R subunit beta; IL-6RB; IL-6R-beta; IL6ST; interleukin 6 signal transducer (gp130, oncostatin M receptor); interleukin receptor beta chain; interleukin-6 receptor subunit beta; Interleukin-6 signal transducer; Membrane glycoprotein 130; membrane glycoprotein gp130; Oncostatin-M receptor subunit alpha

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Citations for Mouse gp130 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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  1. Modulation of Pro-Inflammatory IL-6 Trans-Signaling Axis by Splice Switching Oligonucleotides as a Therapeutic Modality in Inflammation
    Authors: Gupta, D;Orehek, S;Turunen, J;O'Donovan, L;Gait, MJ;El-Andaloussi, S;Wood, MJA;
    Cells
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. TNFR-Associated Factors 2 and 5 Differentially Regulate the Instructive IL-6 Receptor Signaling Required for Th17 Development
    Authors: H Nagashima, Y Okuyama, T Hayashi, N Ishii, T So
    J Immunol, 2016-04-13;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. MicroRNA-146a regulates ICOS-ICOSL signalling to limit accumulation of T follicular helper cells and germinal centres.
    Authors: Pratama, Alvin, Srivastava, Monika, Williams, Naomi J, Papa, Ilenia, Lee, Sau K, Dinh, Xuyen T, Hutloff, Andreas, Jordan, Margaret, Zhao, Jimmy L, Casellas, Rafael, Athanasopoulos, Vicki, Vinuesa, Carola G
    Nat Commun, 2015-03-06;6(0):6436.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6.
    Authors: Nagashima, Hiroyuki, Okuyama, Yuko, Asao, Atsuko, Kawabe, Takeshi, Yamaki, Satoshi, Nakano, Hiroyasu, Croft, Michael, Ishii, Naoto, So, Takanori
    Nat Immunol, 2014-03-30;15(5):449-56.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. IL-6 mediates the susceptibility of glycoprotein 130 hypermorphs to Toxoplasma gondii.
    J. Immunol., 2011-05-23;187(1):350-60.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Modulation of cytokine receptors by IL-2 broadly regulates differentiation into helper T cell lineages.
    Authors: Liao W, Lin JX, Wang L, Li P, Leonard WJ
    Nat. Immunol., 2011-04-24;12(6):551-9.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse gp130 APC-conjugated Antibody
By Anonymous on 02/09/2018
Application: Flow Sample Tested: NIH-3T3 mouse embryonic fibroblast cell line Species: Mouse