Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse, Canine

Applications

Validated:

Immunohistochemistry, Western Blot, Immunoprecipitation

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, Immunocytochemistry, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all HAI-1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603

Specificity

Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Applications for Mouse HAI‑1 Antibody

Application
Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Perfusion fixed frozen sections of mouse intestine

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Mouse HAI‑1 (Catalog # 1141-PI), see our available Western blot detection antibodies

Western Blot

0.1 µg/mL
Sample: Recombinant Mouse HAI‑1 (Catalog # 1141-PI)

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: HAI-1

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

References

  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.

Long Name

HGF Activator Inhibitor Type 1

Alternate Names

HAI1, SPINT1

Entrez Gene IDs

6692 (Human); 20732 (Mouse)

Gene Symbol

SPINT1

UniProt

NP_058603

Additional HAI-1 Products

Product Documents for Mouse HAI‑1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse HAI‑1 Antibody

For research use only

Related Research Areas

Serine Proteases and Regulators

Citations for Mouse HAI‑1 Antibody

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Protocols

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