Mouse HAI-1 Antibody

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Product Details
Citations (9)
Supplemental Products

Mouse HAI-1 Antibody Summary

Species Reactivity
Detects mouse HAI-1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant human HAI‑1 is observed and less than 1% cross-reactivity with recombinant mouse HAI-2B is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse HAI-1
Glu30-Glu443 (Cys325Ser)
Accession # NP_058603
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse HAI‑1 (Catalog # 1141-PI)
5-15 µg/mL
Perfusion fixed frozen sections of mouse intestine
25 µg/mL
Conditioned cell culture medium spiked with Recombinant Mouse HAI‑1 (Catalog # 1141-PI), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: HAI-1

Hepatocyte growth factor activator inhibitor-1 (HAI-1) encoded by the Spint1 gene is a Kunitz-type serine protease inhibitor, identified as a strong inhibitor of HGF activator (HGFA) and matripase (1). The membrane-anchored HAI-1 consists of two Kunitz domains, a LDL-receptor-like domain, and a C-terminal transmembrane domain (2). Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is not only an inhibitor but also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface (3). The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12-myristate, 13-acetate or IL-1 beta. The regulated shedding is completely inhibited by a synthetic zinc metalloprotease inhibitor (3).

  1. Denda, et al. (2002) J. Biol. Chem. 277:14053.
  2. Shimomura, et al. (1997) J. Biol. Chem. 272:6370.
  3. Kataoka, et al. (2000) J. Biol. Chem. 275:40453.
Long Name
HGF Activator Inhibitor Type 1
Entrez Gene IDs
6692 (Human); 20732 (Mouse)
Alternate Names
HAI; HAI1; HAI-1; hepatocyte growth factor activator inhibitor 1; Hepatocyte growth factor activator inhibitor type 1; kunitz-type protease inhibitor 1; MANSC2; serine peptidase inhibitor, Kunitz type 1; serine protease inhibitor, Kunitz type 1; SPINT1

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Citations for Mouse HAI-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Proteolytic Cleavage of Podocin by Matriptase Exacerbates Podocyte Injury
    Authors: S Ozawa, M Matsubayas, H Nanaura, M Yanagita, K Mori, K Asanuma, N Kajiwara, K Hayashi, H Ohashi, M Kasahara, H Yokoi, H Kataoka, E Mori, T Nakagawa
    J. Biol. Chem., 2020-09-09;0(0):.
    Species: Human, Mouse
    Sample Types: Cell Lysates, Whole Tissue
    Applications: IHC, Western Blot
  2. Matriptase Cleaves EpCAM and TROP2 in Keratinocytes, Destabilizing Both Proteins and Associated Claudins
    Authors: CJ Wu, M Lu, X Feng, G Nakato, MC Udey
    Cells, 2020-04-21;9(4):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  3. PDZ-RhoGEF and LARG are essential for embryonic development and provide a link between thrombin and LPA receptors and Rho activation.
    Authors: Mikelis C, Palmby T, Simaan M, Li W, Szabo R, Lyons R, Martin D, Yagi H, Fukuhara S, Chikumi H, Galisteo R, Mukouyama Y, Bugge T, Gutkind J
    J Biol Chem, 2013-03-06;288(17):12232-43.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  4. Hepatocyte growth factor activator inhibitor type 1 is a suppressor of intestinal tumorigenesis.
    Authors: Hoshiko S, Kawaguchi M, Fukushima T, Haruyama Y, Yorita K, Tanaka H, Seiki M, Inatsu H, Kitamura K, Kataoka H
    Cancer Res, 2013-02-27;73(8):2659-70.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC, Western Blot
  5. Reduced prostasin (CAP1/PRSS8) activity eliminates HAI-1 and HAI-2 deficiency-associated developmental defects by preventing matriptase activation.
    Authors: Szabo, Roman, Uzzun Sales, Katiuchi, Kosa, Peter, Shylo, Natalia, Godiksen, Sine, Hansen, Karina K, Friis, Stine, Gutkind, J Silvio, Vogel, Lotte K, Hummler, Edith, Camerer, Eric, Bugge, Thomas H
    PLoS Genet, 2012-08-30;8(8):e1002937.
    Species: Mouse
    Sample Types: Tissue Homogenates, Whole Tissue
    Applications: IHC-P, Immunoprecipitation
  6. Imbalance in the pro-hepatocyte growth factor activation system in bleomycin-induced lung fibrosis in mice.
    Authors: Phin S, Marchand-Adam S, Fabre A, Marchal-Somme J, Bantsimba-Malanda C, Kataoka H, Soler P, Crestani B
    Am. J. Respir. Cell Mol. Biol., 2009-05-15;42(3):286-93.
    Species: Mouse
    Sample Types: BALF, Whole Tissue
    Applications: IHC-P, Neutralization
  7. Potent Inhibition and Global Co-localization Implicate the Transmembrane Kunitz-type Serine Protease Inhibitor Hepatocyte Growth Factor Activator Inhibitor-2 in the Regulation of Epithelial Matriptase Activity.
    Authors: Szabo R, Hobson JP, List K, Molinolo A, Lin CY, Bugge TH
    J. Biol. Chem., 2008-08-19;283(43):29495-504.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P
  8. Hepatocyte growth factor activator inhibitor-1 has a complex subcellular itinerary.
    Authors: Godiksen S, Selzer-Plon J, Pedersen ED, Abell K, Rasmussen HB, Szabo R, Bugge TH, Vogel LK
    Biochem. J., 2008-07-15;413(2):251-9.
    Species: Canine
    Sample Types: Whole Cells
    Applications: ICC
  9. Matriptase inhibition by hepatocyte growth factor activator inhibitor-1 is essential for placental development.
    Authors: Szabo R, Molinolo A, List K, Bugge TH
    Oncogene, 2006-09-18;26(11):1546-56.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P


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Isotype Controls

Reconstitution Buffers

Secondary Antibodies

Goat IgG (H+L) Antibody


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