Mouse Hepcidin Antibody

Catalog # Availability Size / Price Qty
MAB9505-100
MAB9505-SP
Product Details
FAQs
Supplemental Products
Reviews

Mouse Hepcidin Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Hepcidin in direct ELISA.
Source
Monoclonal Rat IgG2A Clone # 968001
Purification
Protein A or G purified from cell culture supernatant
Immunogen
Synthetic peptide containing mouse Hepcidin
Accession # Q9EQ21
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
ELISA

This antibody functions as an ELISA detection antibody when paired with Rat Anti-Mouse Hepcidin Monoclonal Antibody (Catalog # MAB95051).

This product is intended for assay development on various assay platforms requiring antibody pairs.

 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Hepcidin

Hepcidin, also known as Liver Expressed Antimicrobial Protein 1 (LEAP-1), is a peptide hormone that is involved in the regulation of iron metabolism (1, 2). It is synthesized as a preprohormone that is cleaved intracellularly and secreted as a mature 25 amino acid peptide (1, 3, 4). Hepcidin contains eight cysteine residues that form four disulfide bonds which appear to be important for stability in biological fluids (5). It is predominantly expressed, processed, and secreted by hepatocytes (2, 6). Hepcidin expression is positively regulated by inflammation via IL-6/JAK2/ STAT3 signaling, endoplasmic reticulum stress, and BMP-6 (7-11). BMP-6-dependent Hepcidin induction involves RGM-C/Hemojuvelin, which acts as a co-receptor for BMP-6 (11-13). Conversely, Hepcidin expression is negatively regulated by MMP‑15/MT2‑MMP and multiple erythropoietic stimuli, including anemia, hypoxia, and Erythropoietin (14-18). MMP-15 downregulates Hepcidin expression by interacting with and cleaving RGM-C (19). Hepcidin was originally identified in human blood and urine as an antimicrobial peptide (1, 3). It has since been shown to regulate iron metabolism. Hepcidin binds the cellular iron exporter Ferroportin, and this interaction results in Ubiquitin-mediated degradation of both Hepcidin and Ferroportin (20-22). Degradation of Ferroportin results in reduced iron release from macrophages, hepatocytes, and duodenal enterocytes, suggesting that Hepcidin may be an effector of inflammatory hypoferremia (20).

Long Name
Hepcidin Antimicrobial Peptide
Entrez Gene IDs
57817 (Human); 84506 (Mouse); 84604 (Rat)
Alternate Names
HAMP; HEPC; hepcidin antimicrobial peptide; Hepcidin; HEPCPutative liver tumor regressor; HFE2B; HFE2Bhepcidin; LEAP1; LEAP-1; LEAP1PLTR; Liver-expressed antimicrobial peptide 1; PLTR

Product Datasheets

You must select a language.

x

FAQs

  1. What is the specific peptide sequence used as the immunogen for the anti-mouse Hepcidin antibodies, Catalog #s  MAB9505 and MAB95051? 

    • The immunogen used was a synthetic peptide containing mouse Hepcidin (accession # Q9EQ21). A peptide in the C-terminus of this sequence was used. 

View all Antibody FAQs
Loading...

Reviews for Mouse Hepcidin Antibody

There are currently no reviews for this product. Be the first to review Mouse Hepcidin Antibody and earn rewards!

Have you used Mouse Hepcidin Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review