Mouse IFN-alpha Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Mouse IFN-alpha
  • Source
    Polyclonal Rabbit Serum
  • Purification
    Antigen Affinity-purified
  • Immunogen
    E. coli-derived recombinant mouse IFN-alpha A
  • Formulation
    Neat serum diluted in phosphate buffered saline (PBS)
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Neutralization
    One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Shipping
    The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For
    long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the
    contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
    Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers
    containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
  • Long Name:
    Interferon alpha
  • Entrez Gene IDs:
    3440 (Human)
  • Alternate Names:
    IFNalpha; IFN-alpha
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. Identification of Toll-like receptor 9 as parapoxvirus ovis-sensing receptor in plasmacytoid dendritic cells.
    Authors: von Buttlar H, Siegemund S, Buttner M, Alber G
    PLoS ONE, 2014;9(8):e106188.
    Species: Mouse
    Sample Type: Cell Culture Supernates
    Application: ELISA Development
  2. A role for Stat1 in the regulation of lipopolysaccharide-induced interleukin-1beta expression.
    Authors: Joshi VD, Kalvakolanu DV, Chen W, Zhang L, Kang TJ, Thomas KE, Vogel SN, Cross AS
    J. Interferon Cytokine Res., 2006;26(10):739-47.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development

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