Interferon-alpha (IFN-alpha), also known as leukocyte interferon, represents a group of related but distinct proteins that share over 95% amino acid sequence homology. They are members of the type I interferon family which share a common cell surface receptor composed of two subunits, a 100 kDa ligand-binding subunit (IFN-alpha R2) and a 125 kDa ligand binding and signal transduction subunit (IFN-alpha R1) that is involved both in ligand binding and signal transduction. IFN-alpha has both anti-viral and immunomodulatory activities on target cells.
Mouse IFN‑ alpha Antibody
R&D Systems | Catalog # 32100-1
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Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Neutralization
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Polyclonal Rabbit Serum
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Product Specifications
Immunogen
E. coli-derived recombinant mouse IFN-alpha A
Specificity
Mouse IFN-alpha
Clonality
Polyclonal
Host
Rabbit
Isotype
Serum
Applications for Mouse IFN‑ alpha Antibody
Application
Recommended Usage
Neutralization
One Neutralization Unit (NU) is the amount of antiserum required to neutralize one unit of mouse interferon alpha (Mu-IFN-alpha ) to a 50% endpoint. Interferon was titrated with the use of the cytopathic effect inhibition assay using L929 cells as described [Rubinstein, S., Familletti, P.C., and Pestka, S. (1981) "Convenient Assay for Interferons," J. Virol. 37, 755-758] with the exception that EMCV was used as the challenge virus. In this antiviral assay for interferon, about 5 unit/ml of interferon is the quantity necessary to produce a cytopathic effect of 50%. The units are determined with respect to the international reference standard for Mu-IFN-alpha provided by the National Institutes of Health [Ga02-901-511). This material is prepared specifically for effective neutralization of Mu-IFN-alpha.
Formulation, Preparation, and Storage
Formulation
Neat serum diluted in phosphate buffered saline (PBS)
Shipping
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
After receipt, the product may be stored at -20°C for short-term use (≤ 6 months). For long-term storage, we recommend storing the product at -70°C or below for retention of full activity. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum. Refreezing should be done on dry ice or in a dry ice/alcohol bath. Further dilution of the product should be in buffers containing protein such as 0.1% bovine serum albumin (BSA).
Background: IFN-alpha
Long Name
Interferon alpha
Alternate Names
IFNalpha, LeIF D
Entrez Gene IDs
3440 (Human)
Gene Symbol
IFNA2
Additional IFN-alpha Products
Product Documents for Mouse IFN‑ alpha Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IFN‑ alpha Antibody
For research use only
Citations for Mouse IFN‑ alpha Antibody
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