Mouse IFN-gamma ELISpot Development Module, 5 Plate

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SEL485
R&D Systems ELISpot Development Modules
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Mouse IFN-gamma ELISpot Development Module, 5 Plate Summary

Assay Type
Quantitative Sandwich ELISA Development Module
Format
96-well microplate, sold separately (see Other Reagents Required)
Assay Length
3 hours 35 mins to 4 hours 50 mins**
Sample Type
Whole Cells
Sufficient Materials
For five 96-well microplates
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


PRODUCT SUMMARY
Complete ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. Kits are available for detection and enumeration of a single analyte or two analytes simultaneously. Complete ELISpot kits are ready-to-run and require no assay development or refinement. ELISpot Development Modules contain the basic components required to develop an ELISpot assay. They offer an economical alternative to buying separate antibodies.

ELISpot development modules are an alternative to ELISpot kits. A basic understanding of ELISpot assay development is required for the successful use of these reagents. Each investigator should optimize the coating conditions, the assay sensitivity, the type of enzyme and substrate, as well as the concentrations of the capture and detection antibodies to achieve desired results. The analyte-specific ELISpot Development Module and the ELISpot Blue Color Module contain the necessary components for analyte detection and visualization, respectively. These modules can be used together but are sold separately. Each module contains enough reagents for at least five 96-well microplates.


PRODUCT FEATURES

  • An economical alternative to ELISpot Kits
  • Optimized capture and detection antibody pairings and recommended concentrations save lengthy development time
  • Generic development protocols provide direction to start an optimization protocol
  • Customize the assay to your specific needs


MODULE CONTENTS

  • Mouse IFN-gamma Capture Antibody
  • Mouse IFN-gamma Biotinylated-conjugated Detection Antibody


OTHER REAGENTS REQUIRED

  • ELISpot Blue Color Module or equivalent (R&D Systems, Catalog # SEL002)
  • PBS - 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na2HPO4, 1.5 mM KH2PO4, pH 7.2 - 7.4, 0.2 µm filtered
  • Wash Buffer - 0.05% Tween® 20 in PBS
  • Blocking Buffer - 1% BSA, 5% Sucrose in PBS
  • Reagent Diluent - 1% BSA in PBS, pH 7.2 - 7.4, 0.2 µm filtered
  • 2 °C – 8 °C refrigerator
  • 37 °C CO2 incubator
  • Positive Control - Use Recombinant Mouse IFN-gamma or cells known to secrete Mouse IFN-gamma
  • 96-well plates - Nitrocellulose-bottom plates, PVDF-bottom Immunospot® plates, or flat-bottom polystyrene Immulon® ELISA plates
  • Multi-channel pipette, squirt bottle, manifold dispenser, or automated microplate washer
  • Dissection microscope or an automated ELISpot Reader
  • Deionized H2O

Trademarks and registered trademarks are the property of their respective companies.

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at -20 to -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Mouse IFN-gamma ELISpot Development Module, 5 Plate

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

30 Citations: Showing 1 - 10
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  1. Orientation of Antigen Display on Self-Assembling Protein Nanoparticles Influences Immunogenicity
    Authors: CG Schneider, JA Taylor, MQ Sibilo, K Miura, KL Mallory, C Mann, C Karch, Z Beck, GR Matyas, CA Long, E Bergmann-L, P Burkhard, E Angov
    Vaccines, 2021;9(2):.
    Species: Mouse
    Sample Types: Whole Cells
  2. Modifying bacterial flagellin to evade Nod-like Receptor CARD 4 recognition enhances protective immunity against Salmonella
    Authors: P Tourlomous, JA Wright, AS Bittante, LJ Hopkins, SJ Webster, OJ Bryant, P Mastroeni, DJ Maskell, CE Bryant
    Nat Microbiol, 2020;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  3. Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases
    Authors: S Santos, M Ramírez, E Miranda, N Reyes, O Martínez, M Acosta-San, JM Rivera, M Otero
    J Immunol Res, 2019;2019(0):3409371.
    Species: Mouse
    Sample Types: Whole Cells
  4. Preclinical evaluation of multi antigenic HCV DNA vaccine for the prevention of Hepatitis C virus infection
    Authors: H Lee, M Jeong, J Oh, Y Cho, X Shen, J Stone, J Yan, Z Rothkopf, AS Khan, BM Cho, YK Park, DB Weiner, WC Son, JN Maslow
    Sci Rep, 2017;7(0):43531.
    Species: Mouse
    Sample Types: Whole Cells
  5. Critical role for IL-18 in spontaneous lung inflammation caused by autophagy deficiency.
    Authors: Abdel Fattah E, Bhattacharya A, Herron A, Safdar Z, Eissa N
    J Immunol, 2015;194(11):5407-16.
    Species: Mouse
    Sample Types: Whole Cells
  6. Assessing the role of STAT3 in DC differentiation and autologous DC immunotherapy in mouse models of GBM.
    Authors: Assi H, Espinosa J, Suprise S, Sofroniew M, Doherty R, Zamler D, Lowenstein P, Castro M
    PLoS ONE, 2014;9(5):e96318.
    Species: Mouse
    Sample Types: Whole Cells
  7. Inhibition of neointima formation through DNA vaccination for apolipoprotein(a): a new therapeutic strategy for lipoprotein(a).
    Authors: Kyutoku, Mariko, Nakagami, Hironori, Koriyama, Hiroshi, Nakagami, Futoshi, Shimamura, Munehisa, Kurinami, Hitomi, Tomioka, Hideki, Miyake, Takashi, Katsuya, Tomohiro, Morishita, Ryuichi
    Sci Rep, 2013;3(0):1600.
    Species: Mouse
    Sample Types: Whole Cells
  8. Nanoparticle vaccines encompassing the respiratory syncytial virus (RSV) G protein CX3C chemokine motif induce robust immunity protecting from challenge and disease.
    Authors: Jorquera P, Choi Y, Oakley K, Powell T, Boyd J, Palath N, Haynes L, Anderson L, Tripp R
    PLoS ONE, 2013;8(9):e74905.
    Species: Mouse
    Sample Types: Whole Cells
  9. HIV-1 Env DNA vaccine plus protein boost delivered by EP expands B- and T-cell responses and neutralizing phenotype in vivo.
    Authors: Muthumani, Kar, Wise, Megan C, Broderick, Kate E, Hutnick, Natalie, Goodman, Jonathan, Flingai, Seleeke, Yan, Jian, Bian, Chaoran, Mendoza, Janess, Tingey, Colleen, Wilson, Christin, Wojtak, Krzyszto, Sardesai, Niranjan, Weiner, David B
    PLoS ONE, 2013;8(12):e84234.
    Species: Mouse
    Sample Types: Whole Cells
  10. Estriol generates tolerogenic dendritic cells in vivo that protect against autoimmunity.
    Authors: Papenfuss TL, Powell ND, McClain MA, Bedarf A, Singh A, Gienapp IE, Shawler T, Whitacre CC
    J. Immunol., 2011;186(6):3346-55.
    Species: Mouse
    Sample Types: Whole Cells
  11. Desiccating stress promotion of Th17 differentiation by ocular surface tissues through a dendritic cell-mediated pathway.
    Authors: Zheng X, De Paiva CS, Li DQ, Farley WJ, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2010;51(6):3083-91.
    Species: Mouse
    Sample Types: Whole Cells
  12. Plasmids encoding the mucosal chemokines CCL27 and CCL28 are effective adjuvants in eliciting antigen-specific immunity in vivo.
    Authors: Kutzler MA, Kraynyak KA, Nagle SJ, Parkinson RM, Zharikova D, Chattergoon M, Maguire H, Muthumani K, Ugen K, Weiner DB
    Gene Ther., 2010;17(1):72-82.
    Species: Mouse
    Sample Types: Whole Cells
  13. Impact of the underlying mutation and the route of vector administration on immune responses to factor IX in gene therapy for hemophilia B.
    Authors: Cao O, Hoffman BE, Moghimi B, Nayak S, Cooper M, Zhou S, Ertl HC, High KA, Herzog RW
    Mol. Ther., 2009;17(10):1733-42.
    Species: Mouse
    Sample Types: Whole Cells
  14. Transgenic expression of human gp100 and RANTES at specific time points for suppression of melanoma.
    Authors: Aravindaram K, Yu HH, Lan CW, Wang PH, Chen YH, Chen HM, Yagita H, Yang NS
    Gene Ther., 2009;16(11):1329-39.
    Species: Mouse
    Sample Types: Whole Cells
  15. Plasmid-encoded interleukin-15 receptor alpha enhances specific immune responses induced by a DNA vaccine in vivo.
    Authors: Kraynyak KA, Kutzler MA, Cisper NJ, Laddy DJ, Morrow MP, Waldmann TA, Weiner DB
    Hum. Gene Ther., 2009;20(10):1143-56.
    Species: Mouse
    Sample Types: Whole Cells
  16. Immunogenicity of novel consensus-based DNA vaccines against Chikungunya virus.
    Authors: Muthumani K, Lankaraman KM, Laddy DJ, Sundaram SG, Chung CW, Sako E, Wu L, Khan A, Sardesai N, Kim JJ, Vijayachari P, Weiner DB
    Vaccine, 2008;26(40):5128-34.
    Species: Mouse
    Sample Types: Whole Cells
  17. Immunization against the transgene but not the TetON switch reduces expression from gutless adenoviral vectors in the brain.
    Authors: Xiong W, Candolfi M, Kroeger KM, Puntel M, Mondkar S, Larocque D, Liu C, Curtin JF, Palmer D, Ng P, Lowenstein PR, Castro MG
    Mol. Ther., 2008;16(2):343-51.
    Species: Mouse
    Sample Types: Whole Cells
  18. Mucosal delivery of a transmission-blocking DNA vaccine encoding Giardia lamblia CWP2 by Salmonella typhimurium bactofection vehicle.
    Authors: Abdul-Wahid A, Faubert G
    Vaccine, 2007;25(50):8372-83.
    Species: Mouse
    Sample Types: Whole Cells
  19. IL-7 induces myelopoiesis and erythropoiesis.
    Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
    J. Immunol., 2007;178(3):1553-63.
    Species: Mouse
    Sample Types: Whole Cells
  20. Improvement of sublingual immunotherapy efficacy with a mucoadhesive allergen formulation.
    Authors: Razafindratsita A, Saint-Lu N, Mascarell L, Berjont N, Bardon T, Betbeder D, Van Overtvelt L, Moingeon P
    J. Allergy Clin. Immunol., 2007;120(2):278-85.
    Species: Mouse
    Sample Types: Whole Cells
  21. Vaccination with recombinant fusion proteins incorporating Toll-like receptor ligands induces rapid cellular and humoral immunity.
    Authors: Huleatt JW, Jacobs AR, Tang J, Desai P, Kopp EB, Huang Y, Song L, Nakaar V, Powell TJ
    Vaccine, 2007;25(4):763-75.
    Species: Mouse
    Sample Types: Whole Cells
  22. Mapping of immune responses following wild-type and mutant ABeta42 plasmid or peptide vaccination in different mouse haplotypes and HLA Class II transgenic mice.
    Authors: Kutzler MA, Cao C, Bai Y, Dong H, Choe PY, Saulino V, McLaughlin L, Whelan A, Choo AY, Weiner DB, Ugen KE
    Vaccine, 2006;24(21):4630-9.
    Species: Mouse
    Sample Types: Whole Cells
  23. Developing an epitope-driven tuberculosis (TB) vaccine.
    Authors: De Groot AS, McMurry J, Marcon L, Franco J, Rivera D, Kutzler M, Weiner D, Martin B
    Vaccine, 2005;23(17):2121-31.
    Species: Mouse
    Sample Types: Whole Cells
  24. Immunization with gp120-depleted whole killed HIV immunogen and a second-generation CpG DNA elicits strong HIV-specific responses in mice.
    Authors: Trabattoni D, Clivio A, Bray DH, Bhagat L, Beltrami S, Maffeis G, Cesana E, Lowry P, Lissoni F, Kandimalla ER, Sullivan T, Agrawal S, Bartholomew R, Clerici M
    Vaccine, 2005;24(9):1470-7.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  25. Multiple-site DNA vaccination enhances immune responses in mice.
    Authors: Gardiner DF, Huang Y, Basu S, Leung L, Song Y, Chen Z, Ho DD
    Vaccine, 2005;24(3):287-92.
    Species: Mouse
    Sample Types: Whole Cells
  26. Co-immunization with plasmid IL-12 generates a strong T-cell memory response in mice.
    Authors: Chattergoon MA, Saulino V, Shames JP, Stein J, Montaner LJ, Weiner DB
    Vaccine, 2004;22(13):1744-50.
    Species: Mouse
    Sample Types: Whole Cells
  27. Single intranasal mucosal Mycobacterium bovis BCG vaccination confers improved protection compared to subcutaneous vaccination against pulmonary tuberculosis.
    Authors: Chen L, Wang J, Zganiacz A, Xing Z
    Infect. Immun., 2004;72(1):238-46.
    Species: Mouse
    Sample Types: Whole Cells
  28. Therapeutic effectiveness of recombinant cancer vaccines is associated with a prevalent T-cell receptor alpha usage by melanoma-specific CD8+ T lymphocytes.
    Authors: De Palma R, Marigo I, Del Galdo F, De Santo C, Serafini P, Cingarlini S, Tuting T, Lenz J, Basso G, Milan G, Zanovello P, Bronte V
    Cancer Res., 2004;64(21):8068-76.
    Species: Mouse
    Sample Types: Whole Cells
  29. Amelioration of graft versus host disease by galectin-1.
    Authors: Baum LG, Blackall DP, Arias-Magallano S, Nanigian D, Uh SY, Browne JM, Hoffmann D, Emmanouilides CE, Territo MC, Baldwin GC
    Clin. Immunol., 2003;109(3):295-307.
    Species: Mouse
    Sample Types: Whole Cells
  30. Absence of the complement anaphylatoxin C3a receptor suppresses Th2 effector functions in a murine model of pulmonary allergy.
    Authors: Drouin SM, Corry DB, Hollman TJ, Kildsgaard J, Wetsel RA
    J. Immunol., 2002;169(10):5926-33.
    Species: Mouse
    Sample Types: Whole Cells

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