Mouse IFN-gamma ELISpot Kit

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XEL485
EL485
R&D Systems ELISpot and FluoroSpot Kits
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Citations (31)
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Mouse IFN-gamma ELISpot Kit Summary

Assay Type
Quantitative Sandwich ELISA
Format
96-well PVDF-backed microplate
Assay Length
3 hours 15 mins to 4 hours 30 mins*
Sample Type
Whole Cells
Specificity
Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.


PRODUCT SUMMARY
ELISpot kits are highly sensitive, microplate-based assays for the detection of cytokine secreting cells. This kit is designed for the detection and enumeration of mouse IFN-gamma. Complete ELISpot kits are ready-to-run and require no assay development or refinement.

This ELISpot assay employs a capture antibody specific for mouse IFN-gamma, pre-coated onto a PVDF-backed microplate. Appropriately stimulated cells are pipetted directly into the wells and the immobilized antibody in the immediate vicinity of the secreting cells binds secreted mouse IFN-gamma. Following wash steps and incubation with a biotinylated detection antibody, alkaline-phosphatase conjugated to streptavidin is added. Unbound enzyme is subsequently removed by washing and a substrate solution (BCIP/NBT) is added. A blue-black colored precipitate forms at the sites of cytokine localization and appears as spots, with each individual spot representing an individual mouse IFN-gamma secreting cell. The spots can be counted with an automated ELISpot reader system or manually using a stereomicroscope.


PRODUCT FEATURES

  • Detect and quantitate individual cells secreting mouse IFN-gamma
  • High sensitivity - ELISpot assays can measure responses with frequencies well below 1 in 100,000 cells
  • No in vitro expansion of cells required
  • High-throughput - ELISpot assays use only a small number of primary cells


KIT CONTENTS

  • Mouse IFN-gamma Microplate
  • Biotinylated Detection Antibody
  • Streptavidin conjugated to Alkaline Phosphatase
  • Dilution Buffers
  • Wash Buffer Concentrate
  • BCIP/NBT Chromogen
  • Mouse IFN-gamma Positive Control

OTHER REAGENTS REQUIRED

  • Pipettes and pipette tips
  • Deionized or distilled water
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • 500 mL graduated cylinder
  • 37 °C CO2 incubator
  • Sterile culture media
  • Dissection microscope or an automated ELISpot reader

Product Datasheets

Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IFN-gamma

IFN-gamma (Interferon-gamma) is the prototype proinflammatory cytokine and is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells. It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects. In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation. IFN-gamma dimers signal through a receptor complex of two IFN-gamma R1 and two IFN-gamma R2 subunits.

Long Name:
Interferon gamma
Entrez Gene IDs:
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names:
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma
⚠ WARNING: This product can expose you to chemicals including methanol, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov.

Citations for Mouse IFN-gamma ELISpot Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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  1. Combination of Photodynamic Therapy and a Flagellin-Adjuvanted Cancer Vaccine Potentiated the Anti-PD-1-Mediated Melanoma Suppression
    Authors: HS Hwang, K Cherukula, YJ Bang, V Vijayan, MJ Moon, J Thiruppath, S Puth, YY Jeong, IK Park, SE Lee, JH Rhee
    Cells, 2020;9(11):.
    Species: Mouse
    Sample Types: Whole Cells
  2. Synthetic mycobacterial molecular patterns partially complete Freund's adjuvant
    Authors: JY Dubé, F McIntosh, JG Zarruk, S David, J Nigou, MA Behr
    Sci Rep, 2020;10(1):5874.
    Species: Mouse
    Sample Types: Whole Cells
  3. Broad and Effective Protection against Staphylococcus aureus Is Elicited by a Multivalent Vaccine Formulated with Novel Antigens
    Authors: J Deng, X Wang, BZ Zhang, P Gao, Q Lin, RY Kao, K Gustafsson, KY Yuen, JD Huang
    mSphere, 2019;4(5):.
    Species: Mouse
    Sample Types: Whole Cells
  4. Immunological Analysis of a CCHFV mRNA Vaccine Candidate in Mouse Models
    Authors: T Aligholipo, K Földes, K Ergünay, H Gurdal, A Bastug, A Ozkul
    Vaccines (Basel), 2019;7(3):.
    Species: Mouse
    Sample Types: Whole Cells
  5. Extracellular overproduction of E7 oncoprotein of Iranian human papillomavirus type 16 by genetically engineered Lactococcus lactis
    Authors: AH Mohseni, S Taghinezha, H Keyvani, V Razavilar
    BMC Biotechnol., 2019;19(1):8.
    Species: Mouse
    Sample Types: Whole Cells
  6. Protection against human papillomavirus type 16-induced tumors in C57BL/6 mice by mucosal vaccination with Lactococcus lactis NZ9000 expressing E6 oncoprotein
    Authors: S Taghinezha, AH Mohseni, H Keyvani, V Razavilar
    Microb. Pathog., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  7. Memory CD4+ T cells enhance B cell responses to drifting influenza immunization
    Authors: E Gage, N Van Hoeven, ND Cauwelaert, SE Larsen, J Erasmus, MT Orr, RN Coler
    Eur. J. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  8. A modified HLA-A*0201-restricted CTL epitope from human oncoprotein (hPEBP4) induces more efficient antitumor responses
    Authors: W Sun, J Shi, J Wu, J Zhang, H Chen, Y Li, S Liu, Y Wu, Z Tian, X Cao, N Li
    Cell. Mol. Immunol., 2018;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  9. Live attenuated Salmonella typhimurium vaccines delivering SaEsxA and SaEsxB via type III secretion system confer protection against Staphylococcus aureus infection
    Authors: C Xu, BZ Zhang, Q Lin, J Deng, B Yu, S Arya, KY Yuen, JD Huang
    BMC Infect. Dis., 2018;18(1):195.
    Species: Mouse
    Sample Types: Whole Cells
  10. Immunogenicity and Protective Efficacy of the DAR-901 Booster Vaccine in a Murine Model of Tuberculosis
    PLoS ONE, 2016;11(12):e0168521.
    Species: Mouse
    Sample Types: Whole Cells
  11. Transient Expression of Transgenic IL-12 in Mouse Liver Triggers Unremitting Inflammation Mimicking Human Autoimmune Hepatitis
    J Immunol, 2016;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
  12. HIV-1 adenoviral vector vaccines expressing multi-trimeric BAFF and 4-1BBL enhance T cell mediated anti-viral immunity.
    Authors: Kanagavelu, Saravana, Termini, James M, Gupta, Sachin, Raffa, Francesc, Fuller, Katherin, Rivas, Yaelis, Philip, Sakhi, Kornbluth, Richard, Stone, Geoffrey
    PLoS ONE, 2014;9(2):e90100.
    Species: Mouse
    Sample Types: Whole Cells
  13. A whole recombinant yeast-based therapeutic vaccine elicits HBV X, S and Core specific T cells in mice and activates human T cells recognizing epitopes linked to viral clearance.
    Authors: King T, Kemmler C, Guo Z, Mann D, Lu Y, Coeshott C, Gehring A, Bertoletti A, Ho Z, Delaney W, Gaggar A, Subramanian G, McHutchison J, Shrivastava S, Lee Y, Kottilil S, Bellgrau D, Rodell T, Apelian D
    PLoS ONE, 2014;9(7):e101904.
    Species: Mouse
    Sample Types: Whole Cells
  14. Production and immunogenicity of chimeric virus-like particles containing the spike glycoprotein of infectious bronchitis virus.
    Authors: Lv L, Li X, Liu G, Li R, Liu Q, Shen H, Wang W, Xue C, Cao Y
    J Vet Sci, 2014;15(2):209-16.
    Species: Mouse
    Sample Types: Whole Cells
  15. Epidermal RelA specifically restricts contact allergen-induced inflammation and apoptosis in skin.
    Authors: Kumari S, Herzberg B, Pofahl R, Krieg T, Haase I
    J Invest Dermatol, 2014;134(10):2541-50.
    Species: Mouse
    Sample Types: Whole Cells
  16. Vaccination with a fusion protein that introduces HIV-1 gag antigen into a multitrimer CD40L construct results in enhanced CD8+ T cell responses and protection from viral challenge by vaccinia-gag.
    Authors: Gupta, Sachin, Termini, James M, Raffa, Francesc, Williams, Cindi-An, Kornbluth, Richard, Stone, Geoffrey
    J Virol, 2014;88(3):1492-501.
    Species: Mouse
    Sample Types: Whole Cells
  17. Elongated TCR alpha chain CDR3 favors an altered CD4 cytokine profile.
    Authors: Reynolds C, Chong D, Raynsford E, Quigley K, Kelly D, Llewellyn-Hughes J, Altmann D, Boyton R
    BMC Biol, 2014;12(0):32.
    Species: Mouse
    Sample Types: Cell Culture Supernates
  18. The Shc family protein adaptor, Rai, acts as a negative regulator of Th17 and Th1 cell development.
    Authors: Savino, Maria Te, Ulivieri, Cristina, Emmi, Giacomo, Prisco, Domenico, De Falco, Giulia, Ortensi, Barbara, Beccastrini, Enrico, Emmi, Lorenzo, Pelicci, Giuliana, D'Elios, Mario M, Baldari, Cosima T
    J Leukoc Biol, 2013;93(4):549-59.
    Species: Mouse
    Sample Types: Whole Cells
  19. Val-boroPro accelerates T cell priming via modulation of dendritic cell trafficking resulting in complete regression of established murine tumors.
    Authors: Walsh, Meghaan, Duncan, Brynn, Larabee, Shannon, Krauss, Aviva, Davis, Jessica, Cui, Yongzhi, Kim, Su Young, Guimond, Martin, Bachovchin, William, Fry, Terry J
    PLoS ONE, 2013;8(3):e58860.
    Species: Mouse
    Sample Types: Tissue Homogenates
  20. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus.
    Authors: Wu, Chia-Yin, Yeh, Yi-Chun, Chan, Jia-Tsro, Yang, Yu-Chih, Yang, Ji-Rong, Liu, Ming-Tsa, Wu, Ho-Sheng, Hsiao, Pei-Wen
    PLoS ONE, 2012;7(8):e42363.
    Species: Mouse
    Sample Types: Whole Cells
  21. Elicitation of broad CTL response against HIV-1 by the DNA vaccine encoding artificial multi-component fusion protein MultiHIV--study in domestic pigs.
    Authors: Molder T, Adojaan M, Kaldma K, Ustav M, Sikut R
    Vaccine, 2009;28(2):293-8.
    Species: Mouse
    Sample Types: Whole Cells
  22. Novel immunocompetent murine tumor model for evaluation of conditionally replication-competent (oncolytic) murine adenoviral vectors.
    Authors: Robinson M, Li B, Ge Y, Ko D, Yendluri S, Harding T, VanRoey M, Spindler KR, Jooss K
    J. Virol., 2009;83(8):3450-62.
    Species: Mouse
    Sample Types: Whole Cells
  23. Critical role of donor tissue expression of programmed death ligand-1 in regulating cardiac allograft rejection and vasculopathy.
    Authors: Yang J, Popoola J, Khandwala S, Vadivel N, Vanguri V, Yuan X, Dada S, Guleria I, Tian C, Ansari MJ, Shin T, Yagita H, Azuma M, Sayegh MH, Chandraker A
    Circulation, 2008;117(5):660-9.
    Species: Mouse
    Sample Types: Whole Cells
  24. NOD2-deficient mice have impaired resistance to Mycobacterium tuberculosis infection through defective innate and adaptive immunity.
    Authors: Divangahi M, Mostowy S, Coulombe F, Kozak R, Guillot L, Veyrier F, Kobayashi KS, Flavell RA, Gros P, Behr MA
    J. Immunol., 2008;181(10):7157-65.
    Species: Mouse
    Sample Types: Whole Cells
  25. Immunization with adenovirus at the large intestinal mucosa as an effective vaccination strategy against sexually transmitted viral infection.
    Authors: Zhu Q, Thomson CW, Rosenthal KL, McDermott MR, Collins SM, Gauldie J
    Mucosal Immunol, 2008;1(1):78-88.
    Species: Mouse
    Sample Types: Whole Cells
  26. Type I IFNs play a role in early resistance, but subsequent susceptibility, to the African trypanosomes.
    Authors: Lopez R, Demick KP, Mansfield JM, Paulnock DM
    J. Immunol., 2008;181(7):4908-17.
    Species: Mouse
    Sample Types: Whole Cells
  27. Inactivated Sendai virus particles eradicate tumors by inducing immune responses through blocking regulatory T cells.
    Authors: Kurooka M, Kaneda Y
    Cancer Res., 2007;67(1):227-36.
    Species: Mouse
    Sample Types: Whole Cells
  28. One-year Expression From High-capacity Adenoviral Vectors in the Brains of Animals With Pre-existing Anti-adenoviral Immunity: Clinical Implications.
    Authors: Barcia C, Jimenez-Dalmaroni M, Kroeger KM, Puntel M, Rapaport AJ, Larocque D, King GD, Johnson SA, Liu C, Xiong W, Candolfi M, Mondkar S, Ng P, Palmer D, Castro MG, Lowenstein PR
    Mol. Ther., 2007;15(12):2154-2163.
    Species: Mouse
    Sample Types: Whole Cells
  29. Nitric oxide mediates T cell cytokine production and signal transduction in histidine decarboxylase knockout mice.
    Authors: Koncz A, Pasztoi M, Mazan M, Fazakas F, Buzas E, Falus A, Nagy G
    J. Immunol., 2007;179(10):6613-9.
    Species: Mouse
    Sample Types: Whole Cells
  30. Elicitation of immunity to HIV type 1 Gag is determined by Gag structure.
    Authors: Young KR, Ross TM
    AIDS Res. Hum. Retroviruses, 2006;22(1):99-108.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ELISpot Development
  31. Immunopharmacological and antitumor effects of second-generation immunomodulatory oligonucleotides containing synthetic CpR motifs.
    Authors: Wang D, Li Y, Yu D, Song SS, Kandimalla ER, Agrawal S
    Int. J. Oncol., 2004;24(4):901-8.
    Species: Mouse
    Sample Types: Whole Cells

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Mouse IFN-gamma ELISpot Kit
By Anonymous on 10/27/2016
Application: Sample Tested: Mouse splenocytes

http://pubs.acs.org/doi/abs/10.1021/jacs.6b05675