The high-affinity IFN-gamma receptor complex is made up of two type I membrane proteins, IFN-gamma R1 and IFN-gamma R2. Both proteins are members of the type II cytokine receptor family. IFN-gamma R1 is the ligand-binding subunit that is necessary and sufficient for IFN-gamma binding and receptor internalization. IFN-gamma R2 is required for signaling but does not bind IFN-gamma by itself.
Mouse IFN‑ gamma R2 Biotinylated Antibody
R&D Systems | Catalog # BAF1185
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot
Label
Biotin
Antibody Source
Polyclonal Goat IgG
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IFN-gamma R2
Ser21-Val243
Accession # NP_032364
Ser21-Val243
Accession # NP_032364
Specificity
Detects mouse IFN-gamma R2 in Western blots. In this format, approximately 5% cross-reactivity with recombinant human IFN-gamma R2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) IFN-gamma R1 and rmIFN-alpha / beta R2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse IFN‑ gamma R2 Biotinylated Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Mouse IFN-gamma R2
Sample: Recombinant Mouse IFN-gamma R2
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IFN-gamma R2
Long Name
Interferon gamma Receptor 2
Alternate Names
AF-1, IFN-gR2, IFNgammaR2, IFNGR2, IFNGT1
Gene Symbol
IFNGR2
UniProt
Additional IFN-gamma R2 Products
Product Documents for Mouse IFN‑ gamma R2 Biotinylated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IFN‑ gamma R2 Biotinylated Antibody
For research use only
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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