Mouse IFN-gamma  R2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IFN‑ gamma R2 in direct ELISAs and Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant human IFN-gamma R2 is observed and less than 1% cross-reactivity with recombinant mouse (rm) IFN‑ gamma  R1 and rmIFN-alpha / beta R2 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IFN-gamma  R2
    Ser21-Val243
    Accession # NP_032364
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IFN-gamma  R2
  • Neutralization
    Measured by its ability to neutralize IFN‑ gamma  R2-mediated inhibition of EMCV-induced cytopathy in the L‑929 mouse fibroblast cell line. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 1 ng/mL Recombinant Mouse IFN‑ gamma.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IFN‑ gamma  Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IFN‑ gamma  R2 Antibody. Recombinant Mouse IFN‑ gamma (Catalog # 485-MI) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the L‑929 mouse fibroblast cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IFN‑ gamma (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti‑Mouse IFN‑ gamma  R2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1185). The ND50 is typically 0.2-0.8 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IFN-gamma R2

IFN‑ gamma  R2 (Interferon gamma receptor 2; also called IFN‑ gamma R beta IFN‑ gamma  RII, or AF1) is a 60‑64 kDa type I transmembrane glycoprotein that is a member of the class II cytokine receptor family of molecules (1). It is widely expressed as part of a preassembled cell surface multimeric complex. In the absence of IFN‑ gamma, the complex contains two each of IFN‑ gamma  R1, R2 and Jak1 molecules (2). Binding of IFN‑ gamma to IFN‑ gamma  R1 recruits Jak2 to IFN‑ gamma  R2 and initiates phosphorylation, STAT1 binding, conformational changes, and transcriptional regulation, which mainly inhibits proliferation and/or promotes apoptosis (2, 3). Mouse IFN‑ gamma  R2 cDNA encodes 332 amino acids (aa), including a signal sequence (aa 1‑27), an extracellular region (ECD, aa 28‑243) with two fibronectin type III domains, a transmembrane sequence (aa 244‑264) and a cytoplasmic tail (aa 265-332) (1, 2). Within the ECD, mouse IFN‑ gamma  R2 shares 80% aa sequence identity with rat IFN‑ gamma  R2, and 49‑55% with human, canine, porcine and bovine IFN‑ gamma  R2. IFN‑ gamma  R1 and R2 must be from the same species for receptor complexes to be active, and human IFN‑ gamma is not active on the mouse IFN‑ gamma receptor complex (1, 2). IFN‑ gamma  R1 is essential for ligand binding and is more constitutively expressed, while IFN‑ gamma  R2 is essential for signaling, and its more limited expression controls cell response to IFN‑ gamma (2, 3). For example, mouse T cell IFN‑ gamma  R2 is down‑regulated during differentiation to subtypes such as Th1 which produce IFN‑ gamma. (3, 4) This allows expansion of activated cells without growth arrest due to paracrine response to IFN‑ gamma. Following expansion, IFN‑ gamma  R2 is re‑expressed to limit the immune reaction (5). IFN‑ gamma  signaling mediates control of intracellular pathogens such as mycobacteria (3, 4, 6). In humans, deficiency of IFN‑ gamma  R2 or other IFN‑ gamma pathway molecules causes the MSMD (mendelian susceptibility to mycobacterial diseases) syndrome (6‑8).

  • References:
    1. Hemmi, S. et al. (1994) Cell 76:803.
    2. Krause, C.D. et al. (2006) Cell Res. 16:55.
    3. Haring, J. S. et al. (2005) J. Immunol. 174:6791.
    4. Tau, G.Z. et al. (2000) J. Exp. Med. 192:977.
    5. Foulds, K.E. et al. (2008) J. Immunol. 180:842.
    6. Rosenzweig, S.D. et al. (2004) J. Immunol. 173:4000.
    7. Filipe-Santos. O. et al. (2006) Semin. Immunol. 18:347.
    8. Zhang, S-Y. et al. (2008) Immunol. Rev. 226:29.
  • Long Name:
    Interferon gamma Receptor 2
  • Entrez Gene IDs:
    3460 (Human); 15980 (Mouse)
  • Alternate Names:
    AF-1; AF-1interferon-gamma receptor beta chain precursor10IFNGT1Interferon gamma receptor accessory factor 1; IFGR2; IFN-gamma R2; IFN-gamma receptor 2; IFNgammaR2; IFN-gamma-R2; IFNGR2; IFN-gR2; IFNGT1; interferon gamma receptor 2 (interferon gamma transducer 1); interferon gamma receptor 2; interferon gamma receptor accessory factor-1; interferon gamma receptor beta chain; Interferon gamma transducer 1
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