Mouse IGFALS/ALS Antibody
R&D Systems | Catalog # MAB1436
Key Product Details
Species Reactivity
Validated:
Mouse
Cited:
Mouse
Applications
Validated:
Western Blot
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG1 Clone # 199410
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFALS/ALS
Thr28-Cys603
Accession # P70389
Thr28-Cys603
Accession # P70389
Specificity
Detects mouse IGFALS/ALS in direct ELISAs and Western blots.
Clonality
Monoclonal
Host
Rat
Isotype
IgG1
Scientific Data Images for Mouse IGFALS/ALS Antibody
Detection of Mouse IGFALS/ALS by Western Blot.
Western blot shows mouse serum. PVDF Membrane was probed with 2 µg/mL of Rat Anti-Mouse IGFALS/ALS Monoclonal Antibody (Catalog # MAB1436) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). Specific bands were detected for the ALS/IGFBP-3 Complex at approximately 140 kDa (as indicated) and ALS at approximately 80 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Immunoblot Buffer Group 1.Applications for Mouse IGFALS/ALS Antibody
Application
Recommended Usage
Western Blot
2 µg/mL
Sample: Mouse serum
Sample: Mouse serum
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: IGFALS/ALS
Long Name
IGF-binding Protein Acid Labile Subunit
Alternate Names
ACLSD, ALS
Gene Symbol
IGFALS
UniProt
Additional IGFALS/ALS Products
Product Documents for Mouse IGFALS/ALS Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse IGFALS/ALS Antibody
For research use only
Related Research Areas
Citations for Mouse IGFALS/ALS Antibody
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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