Mouse IGFBP-1 Antibody

Catalog # Availability Size / Price Qty
MAB1240
MAB1240-SP
Product Details
Citations (4)
FAQs
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Mouse IGFBP-1 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IGFBP-1 in direct ELISAs and Western blots. In Western blots, weak cross-reactivity with recombinant mouse (rm) IGFBP-5 is observed and no cross-reactivity with recombinant human (rh) IGFBP-1, rmIGFBP-2, rmIGFBP-3, rhIGFBP-4, rmIGFBP-6, or rhIGFBP-7 is observed.
Source
Monoclonal Rat IgG2B Clone # 176110
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IGFBP-1
Ala26-Asn272
Accession # P47876
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Mouse IGFBP‑1 (Catalog # 1588-B1) under non-reducing conditions only

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGFBP-1

The insulin-like growth factor binding protein (IGFBP) family consists of six structurally related proteins that bind IGF with high affinity (1, 2). These proteins share conserved cysteine-rich N- and C- terminal regions that participate in IGF binding. IGFBPs regulate the bioavailability of IGFs and modulate their biological activities, both positively and negatively. Some IGFBPs also have intrinsic bioactivity that is IGF-independent. Post-transitional modifications of the IGFBPs, including glycosylation, phosphorylation and proteolysis, influence IGF binding affinities and tissue localization, affecting both the IGF-dependent and independent functions (1, 2). Mouse IGFBP-1 cDNA encodes a 272 amino acid (aa) precursor protein with a putative 25 aa signal peptide (3). Mature mouse IGFBP-1 contains potential phosphorylation and proteolytic cleavage sites, an Arg-Gly-Asp (RGD) integrin receptor recognition sequence, but lacks potential N-linked glycosylation sites. IGFBP‑1 binds equally well to IGF-I and IGF-II. Phosphorylation of human IGFBP-1, but not rat IGFBP-1, increases IGF affinity. Mouse IGFBP-1 shares 67% and 93% aa sequence identity with human and rat IGFBP-1, respectively. IGFBP-1 is expressed in liver, decidua, and kidneys and is the most abundant IGFBP in amniotic fluid. Hepatic production of IGFBP-1 is down-regulated by insulin and up-regulated by glucocorticoids. Circulating IGFBP-1 levels are elevated under various catabolic conditions including bacterial or viral infections, trauma and diabetes (4). IGFBP-1 has been shown to either potentiate or inhibit the activities of IGF in a variety of cells. IGFBP-1, through its RGD motif, also interacts with alpha 5 beta 1 intergrin to stimulate changes in cell adhesion and migration in the absence of IGFs (1, 2).

References
  1. Firth, S.M. and R.C. Baxter (2002) Endocrine Reviews 23:824.
  2. Rajaram, S. et al. (1997) Endocrine Reviews 18:801.
  3. Schuller, A.G. et al. (1994) Mol. Cell. Endocrinol. 104:57.
  4. Lang, C.H. et al. (2003) Endocrinology 144:3922.
Long Name
Insulin-like Growth Factor Binding Protein 1
Entrez Gene IDs
3484 (Human); 16006 (Mouse)
Alternate Names
alpha-pregnancy-associated endometrial globulin; amniotic fluid binding protein; binding protein-25; binding protein-26; binding protein-28; growth hormone independent-binding protein; hIGFBP-1; IBP1; IBP-1; IGF-binding protein 1; IGFBP1; IGFBP-1; IGF-BP25; insulin-like growth factor binding protein 1; insulin-like growth factor-binding protein 1; Placental protein 12; PP12AFBP

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Citations for Mouse IGFBP-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Microparticles derived from human erythropoietin mRNA-transfected mesenchymal stem cells inhibit epithelial-to-mesenchymal transition and ameliorate renal interstitial fibrosis
    Authors: M Lee, SH Kim, JH Jhee, TY Kim, HY Choi, HJ Kim, HC Park
    Stem Cell Res Ther, 2020-09-29;11(1):422.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Protein restriction cycles reduce IGF-1 and phosphorylated Tau, and improve behavioral performance in an Alzheimer's disease mouse model.
    Authors: Parrella E, Maxim T, Maialetti F, Zhang L, Wan J, Wei M, Cohen P, Fontana L, Longo V
    Aging Cell, 2013-03-11;12(2):257-68.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development (Capture)
  3. Endocrine actions of myostatin: systemic regulation of the IGF and IGF binding protein axis.
    Authors: Williams NG, Interlichia JP, Jackson MF
    Endocrinology, 2010-12-08;152(1):172-80.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development
  4. Evidence of a role for insulin-like growth factor binding protein (IGFBP)-3 in metabolic regulation.
    Authors: Yamada PM, Mehta HH, Hwang D
    Endocrinology, 2010-10-06;151(12):5741-50.
    Species: Mouse
    Sample Types: Serum
    Applications: ELISA Development

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