Mouse IgG2B Isotype Control

R&D Systems | Catalog # MAB0042

R&D Systems
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Key Product Details

Species Reactivity

Mouse

Applications

Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 73009
View all IgG2B Isotype Controls »

Product Specifications

Immunogen

E. coli-derived recombinant protein with a C-terminal E-tag (aa sequence GAPVPYPDPLEPR)

Specificity

Recognizes the E-tag peptide. No background staining was detected by immunohistochemistry on a variety of tissues.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Applications for Mouse IgG2B Isotype Control

Application
Recommended Usage

Control

Negative control for immunohistochemistry or immunocytochemistry experiments. Use at the same concentration as the detection antibody.

Reviewed Applications

Read 1 review rated 5 using MAB0042 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

Long Name

Immunoglobulin G2B Fc

Additional IgG2B Products

Product Documents for Mouse IgG2B Isotype Control

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IgG2B Isotype Control

For research use only

Citations for Mouse IgG2B Isotype Control

Customer Reviews for Mouse IgG2B Isotype Control (1)

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  • Mouse IgG2B Isotype Control
    Name: Anonymous
    Application: Block/Neutralize
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/20/2018
    We used this as an Isotype control for our Endothelial Chemotaxis Assay experiments. Conditioned Medium from Breast cancer cells mixed with IgG2B Isotype Control was used to compare Conditioned medium containing cytokines (IL6, IL8, IL1B) and VEGF-A (IgG2B) neutralization antibody cocktail. Cancer Res; 78(5) March 1, 2018 (Fig 6F)
    Mouse IgG2B Isotype Control MAB0042

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