Mouse IgG2B Isotype Control

(6 citations)   
  • Specificity
    Recognizes the E-tag peptide. No background staining was detected by immunohistochemistry on a variety of tissues.
  • Source
    Monoclonal Mouse IgG2B Clone # 73009
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant protein with a C-terminal E-tag (aa sequence GAPVPYPDPLEPR)
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Control
    Negative control for immunohistochemistry or immunocytochemistry experiments. Use at the same concentration as the detection antibody.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IgG2B

R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.

  • Long Name:
    Immunoglobulin G2B Fc
  • Alternate Names:
    IgG2B
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Species
Applications
Sample Type
  1. In situ characterization of intrahepatic non-parenchymal cells in PSC reveals phenotypic patterns associated with disease severity.
    Authors: Berglin L, Bergquist A, Johansson H, Glaumann H, Jorns C, Lunemann S, Wedemeyer H, Ellis E, Bjorkstrom N
    PLoS ONE, 2014;9(8):e105375.
    Species: Human
    Sample Type: Whole Tissue
    Application: Isotype control
  2. Brain-derived neurotrophic factor is increased in serum and skin levels of patients with chronic spontaneous urticaria.
    Authors: Rossing K, Novak N, Mommert S, Pfab F, Gehring M, Wedi B, Kapp A, Raap U
    Clin. Exp. Allergy, 2011;41(10):1392-1399.
    Species: Human
    Sample Type: Whole Tissue
    Application: Ctrl
  3. Developmental differences in the responses of IL-6 and IL-13 transgenic mice exposed to hyperoxia.
    Authors: Choo-Wing R, Nedrelow JH, Homer RJ, Elias JA, Bhandari V
    Am. J. Physiol. Lung Cell Mol. Physiol., 2007;293(1):L142-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Ctrl
  4. Hepatitis C virus (HCV)-specific CD8+ cells produce transforming growth factor beta that can suppress HCV-specific T-cell responses.
    Authors: Alatrakchi N, Graham CS, van der Vliet HJ, Sherman KE, Exley MA, Koziel MJ
    J. Virol., 2007;81(11):5882-92.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  5. S100A11, an dual mediator for growth regulation of human keratinocytes.
    Authors: Sakaguchi M, Sonegawa H, Murata H, Kitazoe M, Futami J, Kataoka K, Yamada H, Huh NH
    Mol. Biol. Cell, 2007;19(1):78-85.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
  6. Susceptibility of human testis to human immunodeficiency virus-1 infection in situ and in vitro.
    Authors: Roulet V, Satie AP, Ruffault A, Le Tortorec A, Denis H, Guist'hau O, Patard JJ, Rioux-Leclerq N, Gicquel J, Jegou B, Dejucq-Rainsford N
    Am. J. Pathol., 2006;169(6):2094-103.
    Species: Human
    Sample Type: Whole Cells
    Application: Ctrl
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