Mouse IL-1 RI PE-conjugated Antibody

(2 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL-1 RI in direct ELISAs and Western blots.
  • Source
    Monoclonal Rat IgG2B Clone # 129304
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
    Leu20-Lys338
    Accession # P13504
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Phycoerythrin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of IL‑1 RI in Mouse CD3+ Splenocytes by Flow Cytometry. Mouse CD3+ splenocytes were stained with Rat Anti-Mouse IL‑1 RI PE‑conjugated Monoclonal Antibody (Catalog # FAB7712P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: IL-1 RI
The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappa B pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1 RII/IL-1 R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.
  • Long Name:
    Interleukin 1 Receptor I
  • Entrez Gene IDs:
    3554 (Human); 16177 (Mouse)
  • Alternate Names:
    CD121 antigen-like family member A; CD121a antigen; CD121a; IL-1 RI; IL1R; IL1R1; IL1RI; IL-1RI; IL1RT1; IL-1RT1; IL-1RT-1; Interleukin 1 Receptor 1; interleukin 1 receptor, type I; interleukin receptor 1; interleukin-1 receptor type 1; p80
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Species
Applications
Sample Type
  1. TLR9 Regulates the NF-?B-NLRP3-IL-1? Pathway Negatively in Salmonella-Induced NKG2D-Mediated Intestinal Inflammation
    Authors: Y Li, M Liu, Z Zuo, J Liu, X Yu, Y Guan, R Zhan, Q Han, J Zhang, R Zhou, R Sun, Z Tian, C Zhang
    J. Immunol., 2017;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. TLR4-Upregulated IL-1? and IL-1RI Promote Alveolar Macrophage Pyroptosis and Lung Inflammation through an Autocrine Mechanism
    Sci Rep, 2016;6(0):31663.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B PE-conjugated Antibody

Ctrl IC013P 2  
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 6
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 4
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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