Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry

Cited:

Flow Cytometry

Label

Fluorescein (Excitation = 488 nm, Emission = 515-545 nm)

Antibody Source

Monoclonal Rat IgG2B Clone # 129304
View all IL-1 RI Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-1 RI
Leu20-Lys338
Accession # P13504

Specificity

Detects mouse IL-1 RI in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Mouse IL‑1 RI Fluorescein‑conjugated Antibody

Detection of IL-1 RI antibody in B220+Mouse Splenocytes antibody by Flow Cytometry.

Detection of IL‑1 RI in B220+Mouse Splenocytes by Flow Cytometry.

B220+mouse splenocytes were stained with Rat Anti-Mouse IL-1 RI Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB7712F, filled histogram) or isotype control antibody (Catalog # IC013F, open histogram). View our protocol for Staining Membrane-associated Proteins.

Applications for Mouse IL‑1 RI Fluorescein‑conjugated Antibody

Application
Recommended Usage

Flow Cytometry

10 µL/106 cells
Sample: B220+ mouse splenocytes

Reviewed Applications

Read 1 review rated 5 using FAB7712F in the following applications:

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer
Spectra Viewer

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-1 RI

The type I IL-1 receptor (IL-1 RI, designated IL-1 R1 and CD121a) is one of at least nine members of the IL-1 R family within the Toll/IL-1 R (TIR) superfamily. IL‑1 RI is an ~80 kDa type I transmembrane (TM) protein that binds the pleiotropic cytokines IL-1 alpha and IL-1 beta, plus the IL-1 Receptor antagonist (IL-1 Ra). Signal transduction requires complex formation with the IL-1 R accessory protein (IL-1 R AcP/IL-1 R3), another type I TM protein. This complex recruits the adaptor protein MyD88 to initiate signaling in the NF kappa B pathway. Mouse IL-1 RI cDNA encodes a 576 amino acid (aa) protein that contains a 19 aa signal sequence, a 319 aa extracellular domain (ECD) with three C2-type Ig-like domains, a 21 aa TM domain and a 217 aa cytoplasmic region with a TIR domain. Mouse IL-1 RI shares 64%, 83%, 60%, 61% and 55% aa identity with human, rat, canine, equine and bovine IL-1 RI, respectively. The role of IL-1 in inflammation is under several levels of control, including expression and activation of IL-1 alpha and IL-1 beta, expression of IL-1 RI and its accessory and adaptor proteins, and negative regulators such as the IL‑receptor family member, IL-1 RII/IL-1 R2. IL-1 RI is expressed predominantly by T cells, fibroblasts, and endothelial cells and mediates acute phase inflammatory responses including fever.

Long Name

Interleukin 1 Receptor I

Alternate Names

CD121a, IL-1RI, IL1R1, IL1RI

Entrez Gene IDs

3554 (Human); 16177 (Mouse)

Gene Symbol

IL1R1

UniProt

P13504

Additional IL-1 RI Products

Product Documents for Mouse IL‑1 RI Fluorescein‑conjugated Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL‑1 RI Fluorescein‑conjugated Antibody

For research use only

Citations for Mouse IL‑1 RI Fluorescein‑conjugated Antibody

Customer Reviews for Mouse IL‑1 RI Fluorescein‑conjugated Antibody (1)

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  • Mouse IL-1 RI Fluorescein-conjugated Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: Splenocytes
    Species: Mouse
    Verified Customer | Posted 01/07/2020
    Mouse IL‑1 RI Fluorescein‑conjugated Antibody FAB7712F

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