Mouse IL-15R alpha Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF551
Product Details
Citations (16)
FAQs
Supplemental Products
Reviews

Mouse IL-15R alpha Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-15 R alpha in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL‑15 R alpha is observed and less than 1% cross-reactivity with rhIL-2 R alpha, recombinant mouse (rm) IL-2 R beta, and rmIL-2 R gamma is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-15 R alpha
Gly33-Lys205
Accession # Q60819
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑15 R alpha Fc Chimera (Catalog # 551-MR)
Flow Cytometry
2.5 µg/106 cells
EL‑4 mouse lymphoblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-15R alpha

Interleukin 15 receptor alpha (IL-15 R alpha ) is a high affinity receptor that specifically binds IL-15 with high affinity and associates as a heterotrimer with the IL-2 receptors beta and gamma subunits to initiate signal transduction. IL-15 R alpha is expressed on a wide variety of T cells and B cells as well as non-lymphoid cells. IL‑15 R alpha is a 58-60 kDa protein that shares structural similarities to the IL-2 R alpha protein. IL-15 R alpha and IL-2 R alpha genes also share similar intron-exon organization and are closely linked on human chromosome 10p14-p15. Human IL-15 R alpha shares 45% amino acid (aa) homology with the mouse form of the receptor. Signaling of IL‑15 can occur in one of three ways; through the heterotrimeric complex of IL-15 R alpha, IL-2 R beta, and IL-2 R gamma c, through the heterodimeric complex of IL-2 receptors beta and gamma common, through a novel 60-65 kDa IL-15 RX subunit found on mast cells. The binding of IL-15 to IL-15 R alpha has been reported to antagonize the TNF‑ alpha -mediated apoptosis in fibroblasts by competing with TNF RI for TRAF2 binding.

References
  1. Anderson, D.M. et al. (1995) J. Biol. Chem. 270:29862.
  2. Bulfone-Paus, S. et al. (1999) FASEB 13:1575.
  3. Waldemann, T.A. and Y. Tagaya (1999) Ann. Rev. Immunol. 17:19.
  4. Dubois, S. et al. (1999) J. Biol. Chem. 274:26978.
Long Name
Interleukin 15 Receptor alpha
Entrez Gene IDs
3601 (Human); 16169 (Mouse); 102121571 (Cynomolgus Monkey)
Alternate Names
CD215 antigen; CD215; IL15 R alpha; IL-15 R alpha; IL-15 receptor subunit alpha; IL-15R alpha; IL15RA; IL-15Ra; IL-15R-alpha; interleukin 15 receptor, alpha; interleukin-15 receptor subunit alpha; MGC104179

Product Datasheets

Citations for Mouse IL-15R alpha Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
Filter your results:

Filter by:

  1. NK Cells Require Cell-Extrinsic and -Intrinsic TYK2 for Full Functionality in Tumor Surveillance and Antibacterial Immunity
    Authors: N Simonovi?, A Witalisz-S, K Meissl, C Lassnig, U Reichart, T Kolbe, M Farlik, C Bock, V Sexl, M Müller, B Strobl
    J. Immunol., 2019;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. IL-15 signaling promotes adoptive effector T-cell survival and memory formation in irradiation-induced lymphopenia
    Cell Biosci, 2016;6(0):30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. IL-15 receptor alpha signaling constrains the development of IL-17-producing gammadelta T cells.
    Authors: Colpitts S, Puddington L, Lefrancois L
    Proc Natl Acad Sci U S A, 2015;112(31):9692-7.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. Different NK cell developmental events require different levels of IL-15 trans-presentation.
    Authors: Lee GA, Liou YH, Wang SW, Ko KL, Jiang ST, Liao NS
    J. Immunol., 2011;187(3):1212-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Development of an IL-15-autocrine CD8 T-cell leukemia in IL-15-transgenic mice requires the cis expression of IL-15R alpha.
    Authors: Sato N, Sabzevari H, Fu S, Ju W, Petrus MN, Bamford RN, Waldmann TA, Tagaya Y
    Blood, 2011;117(15):4032-40.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. IFN-alpha enhances peptide vaccine-induced CD8+ T cell numbers, effector function, and antitumor activity.
    Authors: Sikora AG, Jaffarzad N, Hailemichael Y, Gelbard A, Stonier SW, Schluns KS, Frasca L, Lou Y, Liu C, Andersson HA, Hwu P, Overwijk WW
    J. Immunol., 2009;182(12):7398-407.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Interleukin-15 combined with an anti-CD40 antibody provides enhanced therapeutic efficacy for murine models of colon cancer.
    Authors: Zhang M, Yao Z, Dubois S, Ju W, Muller JR, Waldmann TA
    Proc. Natl. Acad. Sci. U.S.A., 2009;106(18):7513-8.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. IL-2, -7, and -15, but not thymic stromal lymphopoeitin, redundantly govern CD4+Foxp3+ regulatory T cell development.
    Authors: Vang KB, Yang J, Mahmud SA, Burchill MA, Vegoe AL, Farrar MA
    J. Immunol., 2008;181(5):3285-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  9. Antigen controls IL-7Ralpha expression levels on CD8 T cells during full activation or tolerance induction.
    Authors: Hammerbeck CD, Mescher MF
    J. Immunol., 2008;180(4):2107-16.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Functional Assay
  10. A synaptic basis for paracrine interleukin-2 signaling during homotypic T cell interaction.
    Authors: Sabatos CA, Doh J, Chakravarti S, Friedman RS, Pandurangi PG, Tooley AJ, Krummel MF
    Immunity, 2008;29(2):238-48.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  11. Inhibition of tumor growth by NK1.1+ cells and CD8+ T cells activated by IL-15 through receptor beta/common gamma signaling in trans.
    Authors: Rowley J, Monie A, Hung CF, Wu TC
    J. Immunol., 2008;181(12):8237-47.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  12. IL-15 does not affect IEL development in the thymus but regulates homeostasis of putative precursors and mature CD8 alpha alpha+ IELs in the intestine.
    Authors: Lai YG, Hou MS, Hsu YW, Chang CL, Liou YH, Tsai MH, Lee F, Liao NS
    J. Immunol., 2008;180(6):3757-65.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  13. Regulatory T cells control dendritic cell/NK cell cross-talk in lymph nodes at the steady state by inhibiting CD4+ self-reactive T cells.
    Authors: Terme M, Chaput N, Combadiere B, Ma A, Ohteki T, Zitvogel L
    J. Immunol., 2008;180(7):4679-86.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  14. IL-7 and IL-15 differentially regulate CD8+ T-cell subsets during contraction of the immune response.
    Authors: Rubinstein MP, Lind NA, Purton JF, Filippou P, Best JA, McGhee PA, Surh CD, Goldrath AW
    Blood, 2008;112(9):3704-12.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. DAP12 signaling directly augments proproliferative cytokine stimulation of NK cells during viral infections.
    Authors: French AR, Sjolin H, Kim S, Koka R, Yang L, Young DA, Cerboni C, Tomasello E, Ma A, Vivier E, Karre K, Yokoyama WM
    J. Immunol., 2006;177(8):4981-90.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  16. Distinct modulatory effects of LPS and CpG on IL-18-dependent IFN-gamma synthesis.
    Authors: Gould MP, Greene JA, Bhoj V, DeVecchio JL, Heinzel FP
    J. Immunol., 2004;172(3):1754-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse IL-15R alpha Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-15R alpha Biotinylated Antibody and earn rewards!

Have you used Mouse IL-15R alpha Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review