Mouse IL-17F Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC20571R-025
Detection  of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by  Flow Cytometry.
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Mouse IL-17F Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17F in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1058A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-17F
Ala21-Val153
Accession # Q7TNI7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Intracellular Staining by Flow Cytometry Detection  of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by  Flow Cytometry. View Larger

Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL‑17F Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # IC20571R) or (B) Normal Rabbit IgG Alexa Fluor® 647‑conjugated Control.  To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17F

IL-17F, also called ML-1, is a 21-33 kDa member of the IL-17 family of protein. It is one of six glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is expressed by multiple cell types, including bronchial epthelium, neutrophils (likely), monocytes, mast cells, colonic epithelium, gamma δ T cells, CD4+ Th17 cells, group 3 (NKp46- ROR gamma t) innate lymphoid cells, NKT cells and CD8+ Tc17 cells. IL-17F is known to form disulfide-linked homodimers, and covalent heterodimes with IL-17A. Notable, the IL-17A and IL-17F homodimers, plus the IL-17A:F heterodimer use the same receptor combination (IL-17RA:IL-17RC), but elicit somewhat different outcomes. For instance, on macrophages, IL-17A induces IL-9, KC and GM-CSF secretion, while IL-17F does not. Both molecules, however, induce the secretion of the same molecules (IL-9, KC, and GM-CSF) by colonic epithelium. Mouse IL-17F has a long form and a short form, which have alternate start sites but identical lengths. Over amino acids (aa) 21-153, mouse IL-17F shares 88% and 56% aa sequence identity with rat and human IL-17F, respectively.

Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24

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