Mouse IL‑17F Alexa Fluor® 647‑conjugated Antibody

R&D Systems | Catalog # IC20571R

Recombinant Monoclonal Antibody.
R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Intracellular Staining by Flow Cytometry

Label

Alexa Fluor 647 (Excitation = 650 nm, Emission = 668 nm)

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1058A
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Product Specifications

Immunogen

E. coli-derived recombinant mouse IL-17F
Ala21-Val153
Accession # Q7TNI7

Specificity

Detects mouse IL-17F in direct ELISAs.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Mouse IL‑17F Alexa Fluor® 647‑conjugated Antibody

Detection of IL-17F antibody in Mouse Splenocytes Stimulated to Induce Th17 Cells antibody by Flow Cytometry.

Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry.

Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC20571R) or (B) Normal Rabbit IgG Alexa Fluor® 647-conjugated Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). View our protocol for Staining Intracellular Molecules.

Applications for Mouse IL‑17F Alexa Fluor® 647‑conjugated Antibody

Application
Recommended Usage

Intracellular Staining by Flow Cytometry

5 µL/106 cells
Sample: Mouse splenocytes stimulated to induce Th17 cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005).

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Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months, 2 to 8 °C under sterile conditions after opening.
  • Background: IL-17F

    IL-17F, also called ML-1, is a 21-33 kDa member of the IL-17 family of protein. It is one of six glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is expressed by multiple cell types, including bronchial epthelium, neutrophils (likely), monocytes, mast cells, colonic epithelium, gamma delta T cells, CD4+ Th17 cells, group 3 (NKp46- ROR gamma t) innate lymphoid cells, NKT cells and CD8+ Tc17 cells. IL-17F is known to form disulfide-linked homodimers, and covalent heterodimes with IL-17A. Notable, the IL-17A and IL-17F homodimers, plus the IL-17A:F heterodimer use the same receptor combination (IL-17RA:IL-17RC), but elicit somewhat different outcomes. For instance, on macrophages, IL-17A induces IL-9, KC and GM-CSF secretion, while IL-17F does not. Both molecules, however, induce the secretion of the same molecules (IL-9, KC, and GM-CSF) by colonic epithelium. Mouse IL-17F has a long form and a short form, which have alternate start sites but identical lengths. Over amino acids (aa) 21-153, mouse IL-17F shares 88% and 56% aa sequence identity with rat and human IL-17F, respectively.

    Long Name

    Interleukin 17F

    Alternate Names

    IL17F

    Entrez Gene IDs

    112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)

    Gene Symbol

    IL17F

    UniProt

    Additional IL-17F Products

    Product Documents for Mouse IL‑17F Alexa Fluor® 647‑conjugated Antibody

    Certificate of Analysis

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    Note: Certificate of Analysis not available for kit components.

    Product Specific Notices for Mouse IL‑17F Alexa Fluor® 647‑conjugated Antibody


    This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

    For research use only

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