Mouse IL-17F Alexa Fluor® 647-conjugated Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
IC20571R-025
Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry.
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Mouse IL-17F Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17F in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1058A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E. coli-derived recombinant mouse IL-17F
Ala21-Val153
Accession # Q7TNI7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration
Sample
Intracellular Staining by Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of IL-17F antibody in Mouse Splenocytes Stimulated to Induce Th17 Cells antibody by Flow Cytometry. View Larger

Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 Cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE-conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL-17F Alexa Fluor® 647-conjugated Monoclonal Antibody (Catalog # IC20571R) or (B) Normal Rabbit IgG Alexa Fluor® 647-conjugated Control. To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17F

IL-17F, also called ML-1, is a 21-33 kDa member of the IL-17 family of protein. It is one of six glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is expressed by multiple cell types, including bronchial epthelium, neutrophils (likely), monocytes, mast cells, colonic epithelium, gamma δ T cells, CD4+ Th17 cells, group 3 (NKp46- ROR gamma t) innate lymphoid cells, NKT cells and CD8+ Tc17 cells. IL-17F is known to form disulfide-linked homodimers, and covalent heterodimes with IL-17A. Notable, the IL-17A and IL-17F homodimers, plus the IL-17A:F heterodimer use the same receptor combination (IL-17RA:IL-17RC), but elicit somewhat different outcomes. For instance, on macrophages, IL-17A induces IL-9, KC and GM-CSF secretion, while IL-17F does not. Both molecules, however, induce the secretion of the same molecules (IL-9, KC, and GM-CSF) by colonic epithelium. Mouse IL-17F has a long form and a short form, which have alternate start sites but identical lengths. Over amino acids (aa) 21-153, mouse IL-17F shares 88% and 56% aa sequence identity with rat and human IL-17F, respectively.

Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat); 708220 (Rhesus Macaque)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; interleukin 17F

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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