Mouse IL-17F Antibody Summary
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL‑17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
IL-17F, also called ML-1 or IL-24, is one of six IL-17 family members. All are homodimeric glycoproteins with conserved cysteines that form a cystine knot structure. IL-17F is a 44 kDa dimer expressed by activated monocytes that acts in a stimulation loop with activated CD4+T cells to produce IL-2, TGFb and MCP-1. Like IL-17A, it recruits neutrophils to airways during allergic reactions. IL-17F has a long form and a short form (predominant), which have alternate start sites but similar function. Mouse IL-17F is 58% aa identical to human IL-17F.
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