Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 cells by Flow Cytometry.
Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL‑17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 °C, as supplied.
1 month, 2 to 8 °C under sterile conditions after opening.
6 months, -20 to -70 °C under sterile conditions after opening.
IL-17F, also called ML-1 or IL-24, is one of six IL-17 family members. All are homodimeric glycoproteins with conserved cysteines that form a cystine knot structure. IL-17F is a 44 kDa dimer expressed by activated monocytes that acts in a stimulation loop with activated CD4+T cells to produce IL-2, TGFb and MCP-1. Like IL-17A, it recruits neutrophils to airways during allergic reactions. IL-17F has a long form and a short form (predominant), which have alternate start sites but similar function. Mouse IL-17F is 58% aa identical to human IL-17F.
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