Mouse IL-17F Antibody

Recombinant Monoclonal Antibody
Catalog # Availability Size / Price Qty
MAB20571
MAB20571-SP
Detection of IL‑17F in Mouse Splenocytes  Stimulated to Induce Th17 cells by Flow Cytometry.
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Mouse IL-17F Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17F in direct ELISAs.
Source
Recombinant Monoclonal Rabbit IgG Clone # 1058A
Purification
Protein A or G purified from cell culture supernatant
Immunogen
E.coli-derived recombinant mouse IL-17F
Ala21-Val153
Accession # Q7TNI7
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
1:1000 dilution
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL‑17F in Mouse Splenocytes  Stimulated to Induce Th17 cells by Flow Cytometry. View Larger

Detection of IL‑17F in Mouse Splenocytes Stimulated to Induce Th17 cells by Flow Cytometry. Mouse splenocytes stimulated to induce Th17 cells were stained with Rat Anti-Mouse CD4 PE‑conjugated Monoclonal Antibody (Catalog # FAB554P) and either (A) Rabbit Anti-Mouse IL‑17F Monoclonal Antibody (Catalog # MAB20571) or (B) Normal Rabbit IgG Control (Catalog # AB-105-C) followed by Allophycocyanin-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # F0111).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 °C, as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after opening.
  • 6 months, -20 to -70 °C under sterile conditions after opening.

Background: IL-17F

IL-17F, also called ML-1 or IL-24, is one of six IL-17 family members.  All are homodimeric glycoproteins with conserved cysteines that form a cystine knot structure.  IL-17F is a 44 kDa dimer expressed by activated monocytes that acts in a stimulation loop with activated CD4+T cells to produce IL-2, TGFb and MCP-1.  Like IL-17A, it recruits neutrophils to airways during allergic reactions. IL-17F has a long form and a short form (predominant), which have alternate start sites but similar function. Mouse IL-17F is 58% aa identical to human IL-17F.

Long Name
Interleukin 17F
Entrez Gene IDs
112744 (Human); 257630 (Mouse); 301291 (Rat)
Alternate Names
Cytokine ML-1; IL17F; IL-17F; IL-17Finterleukin-17F; IL24; IL-24; interleukin 17F; Interleukin-24; ML-1; ML1interleukin-24

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