Mouse IL-17RB Alexa Fluor® 488-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB10402G-100
FAB10402G-025
Detection of IL‑17 RB in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB by Flow Cytometry.
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Mouse IL-17RB Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-17 RB in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) IL-17 RB, rhIL-17 R,  or recombinant mouse IL-17 is observed.
Source
Monoclonal Rat IgG1 Clone # 752101
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-17 RB
Arg18-Gly286
Accession # Q9JIP3
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
5 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Flow Cytometry Detection of IL-17 RB antibody in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB antibody by Flow Cytometry. View Larger

Detection of IL‑17 RB in CHO Chinese Hamster Cell Line Transfected with Mouse IL-17 RB by Flow Cytometry. CHO Chinese hamster ovary cell line transfected with mouse IL-17 RB was stained with Rat Anti-Mouse IL-17 RB Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB10402G, filled histogram) or isotype control antibody (Catalog # IC005G, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: IL-17RB

The Interleukin 17 (IL-17) family of cytokines, comprising six members (IL-17, IL-17B through IL-17F), are structurally related proteins with a conserved cysteine-knot structure. These proinflammatory cytokines can induce local cytokine production and are involved in the regulation of the immune response. The cognate receptors activated by some of these cytokines have been identified (1, 2). Interleukin-17B Receptor (IL-17 RB), also known as IL-17 Rh1, IL-17E R and EVI27, represents the second receptor of the IL-17 family cytokines to be recognized (2‑4). Mouse IL-17 RB cDNA encodes a 499 amino acid (aa) type I membrane protein with a putative 17 aa signal peptide, a 269 aa extracellular domain, a 21 aa transmembrane domain and a 192 aa cytoplasmic tail. As a result of alternative splicing, a secreted variant of IL-17 RB also exists (5). Human and mouse IL‑17 RB share 76% aa sequence identity. IL-17 RB is approximately 20% identical to the human and mouse IL‑17 R. However, the receptors share many conserved cysteine residues within their extracellular domains as well as additional conserved elements within their cytoplasmic domains. At least three additional type I transmembrane receptors with homology to IL‑17 R, including IL-17 RL (IL-17 RC), IL-17 RD, and IL-17 RE, have been reported (2, 6). By Northern blot analysis, mouse IL-17 RB is highly expressed in liver and testes and is expressed at lower levels in kidney and lung. It is also expressed in some hematopoietic cell lines, including selected T cell, B cell, and myeloid cell lines (2‑4). The expression of IL-17 RB is significantly upregulated under inflammatory conditions (7). IL-17 RB binds strongly to IL-17E and weakly to IL-17B. It does not bind IL-17 or IL-17F. Activation of IL-17 RB by its ligands results in the activation of nuclear factor kappa-B (2‑4).

References
  1. Aggarwal, S. and A.L. Gurney (2002) J. Leukoc. Biol. 71:1.
  2. Moseley, T.A. et al. (2003) Cytokine & Growth Factor Rev. 14:155.
  3. Shi, Y. et al. (2000) J. Biol. Chem. 275:19167.
  4. Lee, J. et al. (2001) J. Biol. Chem. 276:1660.
  5. Tian, E. et al. (2000) Oncogene 19:2098.
  6. Haudenschild, D. et al. (2002) J. Biol. Chem. 277:4309.
  7. Hurst, S.D. et al. (2002) J. Immunol. 169:443.
Long Name
Interleukin 17B Receptor
Entrez Gene IDs
55540 (Human); 50905 (Mouse)
Alternate Names
CRL4IL-17 receptor homolog 1; cytokine receptor CRL4; Cytokine receptor-like 4; Evi27; EVI27IL-17B receptor; IL-17 RB; IL-17 receptor B; IL-17 RH1; IL17BRinterleukin 17B receptor; IL-17ER; IL17RB; IL-17RB; IL17Rh1; IL-17Rh1; IL17RH1MGC5245; interleukin 17 receptor B; interleukin 17 receptor homolog 1; interleukin-17 receptor B; Interleukin-17B receptor

Product Datasheets

Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

Citation for Mouse IL-17RB Alexa Fluor® 488-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Transcriptome and chromatin landscape of iNKT cells are shaped by subset differentiation and antigen exposure
    Authors: MP Murray, I Engel, G Seumois, S Herrera-De, SL Rosales, A Sethi, A Logandha R, GY Seo, J Greenbaum, P Vijayanand, JP Scott-Brow, M Kronenberg
    Nature Communications, 2021;12(1):1446.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Mouse IL-17RB Alexa Fluor® 488-conjugated Antibody
By Anonymous on 05/14/2018
Application: Func Sample Tested: MBA-MB-123 human breast cancer cell line Species: Mouse