Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody

Catalog #: BAF4635
1 Citation Datasheet / COA / SDS

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BAF4635

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Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody Summary

Species Reactivity

Mouse

Specificity

Detects mouse IL‑28A/IFN‑ lambda 2 in Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse IL-28B is observed, less than 10% cross-reactivity with recombinant human (rh) IL-28B is observed, and less than 5% cross-reactivity with rhIL-28A is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL‑28A/IFN‑ lambda 2
Asp20-Val193
Accession # NP_001019844

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Mouse IL‑28A/IFN-lambda 2 (Catalog # 4635-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Reconstitution Buffer Available

Reconstitution Buffer 1 (PBS)

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-28A/IFN-lambda 2

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor beta (IL-10 R beta ) and IL-28 receptor alpha (IL-28 R alpha ; also called IFN-lambda R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).

References

Chen, Q. et al. (2006) Vitam. Horm. 74:207.
Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
Ank, N. et al. (2006) J. Virol. 80:4501.
Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
Siebler, J. et al. (2007) Gastroenterology 132:358.
Meager, A. et al. (2005) Cytokine 31:109.
Lasfar, A. et al. (2006) Cancer Res. 66:4468.
Sato, A. et al. (2006) J. Immunol. 176:7686.
Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.

Long Name

Interleukin 28A

Entrez Gene IDs

282616 (Human)

Alternate Names

interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2

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Citation for Mouse IL-28A/IFN-lambda 2 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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