Mouse IL-28A/IFN-lambda 2 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse IL‑28A/IFN‑ lambda 2 in direct ELISAs and Western blots. In Western blots, approximately 50% cross-reactivity with recombinant mouse IL‑28B is observed and less than 5% cross-reactivity with recombinant human (rh) IL-28A and rhIL-28B is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse IL‑28A/IFN‑ lambda 2
    Asp20-Val193
    Accession # NP_001019844
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse IL‑28A/IFN-lambda 2 (Catalog # 4635-ML)
  • Neutralization
    Measured by its ability to neutralize IL‑28A/IFN‑ lambda 2 inhibition of EMCV-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line. Sheppard, P. et al. (2003) Nat. Immunol. 4:63. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 20 ng/mL Recombinant Mouse IL‑28A/IFN‑ lambda 2.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
IL‑28A/IFN‑ lambda 2 Inhibition of EMCV-induced Cytopathy and Neutralization by Mouse IL‑28A/IFN‑ lambda 2 Antibody. Recombinant Mouse IL‑28A (Catalog # 4635‑ML) reduces the Encephalomyocarditis Virus (EMCV)-induced cytopathy in the HepG2 human hepatocellular carcinoma cell line in a dose-dependent manner (orange line). Inhibition of EMCV activity elicited by Recombinant Mouse IL‑28A (20 ng/mL) is neutralized (green line) by increasing concentrations of Sheep Anti‑Mouse IL‑28A Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4635). The ND50 is typically 3‑15 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: IL-28A/IFN-lambda 2

IL-28A (also named interferon-lambda 2, IFN-lambda 2), IL-28B (IFN-lambda 3) and IL-29 (IFN-lambda 1) are type III interferons that are class II cytokine receptor ligands (1‑4). They are distantly related to members of the IL-10 family and type I IFN family (1‑4). Mouse IL-28A cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28A shares 81% and 66% aa sequence identity with rat and human IL-28A, respectively, and functions across species (5). Mouse IL-28A and IL-28B share 97% aa identity; the mouse lacks a functional IL-29 gene (4). Type III interferons are widely expressed, but are mainly produced by antigen presenting cells in response to viruses and double-stranded RNA that interact with Toll-like receptors or RIG-1 family helicases (2‑6). They signal through a widely expressed receptor that is a heterodimer of the IL-10 receptor  beta (IL-10 R beta ) and IL-28 receptor  alpha (IL-28 R alpha ; also called IFN-lambda  R1) (2, 3, 7, 9). Interaction of either type I or type III IFNs with their receptors activates similar pathways, including JAK tyrosine kinase activation, STAT phosphorylation and formation of the IFN-stimulated regulatory factor 3 (ISGF-3) transcription factor complex (1‑3). Both type I and III IFNs induce antiviral activity and upregulate MHC class I antigen expression (2‑6). Cell lines responsive to type III IFNs are also responsive to type I IFNs, but in general, higher concentrations of type III IFNs are needed for similar in vitro responses (8). In vivo, however, type III IFNs enhance levels of IFN-gamma in serum, suggesting that the robust antiviral activity of type III IFNs may stem in part from activation of the immune system (5, 7). Anti-proliferative and antitumor activity in vivo has also been shown for type III IFNs (9‑11).

  • References:
    1. Chen, Q. et al. (2006) Vitam. Horm. 74:207.
    2. Sheppard, P. et al. (2003) Nat. Immunol. 4:63.
    3. Kotenko, S.V. et al. (2003) Nat. Immunol. 4:69.
    4. Bartlett, N.W. et al. (2005) J. Gen. Virol. 86:1589.
    5. Ank, N. et al. (2006) J. Virol. 80:4501.
    6. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
    7. Siebler, J. et al. (2007) Gastroenterology 132:358.
    8. Meager, A. et al. (2005) Cytokine 31:109.
    9. Lasfar, A. et al. (2006) Cancer Res. 66:4468.
    10. Sato, A. et al. (2006) J. Immunol. 176:7686.
    11. Zitzmann, K. et al. (2006) Biochem. Biophys. Res. Commun. 344:1334.
  • Long Name:
    Interleukin 28A
  • Entrez Gene IDs:
    282616 (Human)
  • Alternate Names:
    interleukin-28A; IFN-lambda 2; IL28A; IL-28A; interferon, lambda 2
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