Mouse IL-3 Quantikine ELISA Kit

(10 citations)   
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (50 uL)
  • Sensitivity
    2.5 pg/mL
  • Assay Range
    7.8 - 500 pg/mL (Cell Culture Supernates, Serum)
  • Specificity
    Natural and recombinant mouse IL-3
  • Cross-reactivity
    < 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
  • Interference
    No significant interference observed with available related molecules.
Product Summary
The Quantikine Mouse IL-3 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse IL-3 in cell culture supernates and mouse serum. It contains E. coli-expressed recombinant mouse IL-3 and antibodies raised against recombinant mouse IL-3. This Immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-3 showed dose curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-3.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum
Intra-Assay Precision Inter-Assay Precision
Standard Deviation1.78.3222.55.518


The recovery of mouse IL-3 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=5) 104 91-114
Serum (n=5) 96 90-106
To assess the linearity of the assay, five or more samples containing and/or spiked with various concentrations of mouse IL-3 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: IL-3
Interleukin-3 (IL-3) is a secreted cytokine that stimulates the proliferation and differentiation of pluripotent hematopoietic stem cells and various lineage-committed progenitors. It also regulates the activation of mature mast cells, basophils, eosinophils, and macrophages. It is produced by activated T cells, thymic epithelial cells, activated mast cells, keratinocytes, neurons, and astrocytes. IL-3 signals through a receptor complex that contains IL-3 R alpha/CD123 and the common beta chain which is also a component of the receptors for IL-5 and GM-CSF. Receptors for IL-3 are present on bone marrow progenitors, macrophages, mast cells, eosinophils, megakaryocytes, basophils, and various myeloid leukemic cells. Human IL-3 does not show activity on mouse cells.
    • Long Name
      Interleukin 3
    • Entrez Gene IDs
      3562 (Human); 16187 (Mouse); 24495 (Rat);
    • Alternate Names
      Hematopoietic growth factor; IL3; IL-3MGC79398; interleukin 3 (colony-stimulating factor, multiple); interleukin-3; Mast cell growth factor; mast-cell growth factor; MCGFMGC79399; MULTI-CSF; multilineage-colony-stimulating factor; Multipotential colony-stimulating factor; P-cell stimulating factor; P-cell-stimulating factor;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 50 µL Assay Diluent
    4.   Add 50 µL of Assay Diluent to each well.

    5. 50 µL Standard, Control, or Sample
    6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
    7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

    8. 100 µL Conjugate
    9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
    10.   Aspirate and wash 5 times.

    11. 100 µL Substrate Solution
    12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.

    13. 100 µL Stop Solution
    14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    Showing Results 1 - 10 of 10
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    Sample Type
    1. Comprehensive Ex Vivo Transposon Mutagenesis Identifies Genes That Promote Growth Factor Independence and Leukemogenesis.
      Authors: Guo Y, Updegraff B, Park S, Durakoglugil D, Cruz V, Maddux S, Hwang T, O'Donnell K
      Cancer Res, 2015;76(4):773-86.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    2. Bcl3 prevents acute inflammatory lung injury in mice by restraining emergency granulopoiesis.
      Authors: Kreisel D, Sugimoto S, Tietjens J, Zhu J, Yamamoto S, Krupnick AS, Carmody RJ, Gelman AE
      J. Clin. Invest., 2011;121(1):265-76.
      Species: Mouse
      Sample Type: Serum
    3. IL-7 induces myelopoiesis and erythropoiesis.
      Authors: Aiello FB, Keller JR, Klarmann KD, Dranoff G, Mazzucchelli R, Durum SK
      J. Immunol., 2007;178(3):1553-63.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    4. Regulatory T cells prevent catastrophic autoimmunity throughout the lifespan of mice.
      Authors: Kim JM, Rasmussen JP, Rudensky AY
      Nat. Immunol., 2007;8(2):191-7.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    5. Suppression of established experimental autoimmune uveitis by anti-LFA-1alpha Ab.
      Authors: Ke Y, Sun D, Zhang P, Jiang G, Kaplan HJ, Shao H
      Invest. Ophthalmol. Vis. Sci., 2007;48(6):2667-75.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    6. A novel polysaccharide of black soybean promotes myelopoiesis and reconstitutes bone marrow after 5-flurouracil- and irradiation-induced myelosuppression.
      Authors: Liao HF, Chen YJ, Yang YC
      Life Sci., 2005;77(4):400-13.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    7. Increased interleukin-10 associated with low IL-6 concentration correlated with greater survival rates in mice infected by rabies virus vaccinated against it and immunomodulated with P. acnes.
      Authors: Megid J, Kaneno R, Nozaki CN, Brito CJ, Almeida MF
      Comp. Immunol. Microbiol. Infect. Dis., 2004;27(6):393-411.
      Species: Mouse
      Sample Type: Serum
    8. The protein tyrosine kinase inhibitor AG126 prevents the massive microglial cytokine induction by pneumococcal cell walls.
      Authors: Hanisch UK, Prinz M, Angstwurm K, Hausler KG, Kann O, Kettenmann H, Weber JR
      Eur. J. Immunol., 2001;31(7):2104-15.
      Species: Mouse
      Sample Type: Cell Culture Supernates
    9. Interleukin 3 and granulocyte-macrophage colony-stimulating factor are not required for induction of chronic myeloid leukemia-like myeloproliferative disease in mice by BCR/ABL.
      Authors: Li S, Gillessen S, Tomasson MH, Dranoff G, Gilliland DG, Van Etten RA
      Blood, 2001;97(5):1442-50.
      Species: Mouse
      Sample Type: Serum
    10. A secreted and LIF-mediated stromal cell-derived activity that promotes ex vivo expansion of human hematopoietic stem cells.
      Authors: Shih CC, Hu MC, Hu J
      Blood, 2000;95(6):1957-66.
      Species: Mouse
      Sample Type: Cell Culture Supernates
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