Mouse IL-36 beta/IL-1F8 Biotinylated Antibody

Catalog # Availability Size / Price Qty
BAF2298
Product Details
Citations (1)
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Mouse IL-36 beta/IL-1F8 Biotinylated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL-36 beta /IL-1F8 in Western blots. In Western blots, less than 2% cross-reactivity with recombinant human (rh) IL-1F8, recombinant mouse (rm) IL-1 alpha, rmIL-1 beta, rmIL-1ra, rmIL-1F6, rhIL-1F7, rhIL-1F9 and rhIL-1F10 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse IL-36 beta /IL-1F8
Met1-Lys183
Accession # Q9D6Z6
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑36 beta /IL‑1F8 (Catalog # 2298-ML)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-36 beta/IL-1F8

Mouse Interleukin-36 beta [IL‑36 beta ; also named  interleukin 1 family member 8, IL-1F8,  FIL-1 eta (eta) and IL-1H2] is a member of the IL-1 family of proteins (1‑3). IL-1 family members include IL‑1 beta, IL-1 alpha, IL-1ra, IL-18 and IL-1F5 through F10 (4). All family members show a 12 beta -stranded beta -trefoil configuration, and are believed to have arisen from a common ancestral gene that has undergone multiple duplications (4). Although two alternatively spliced transcript variants for human IL-1F8 have been described, to date, only one mouse IL-1F8 isoform is known (3). Mouse IL-1F8 is synthesized as a 183 amino acid (aa) protein that contains no signal sequence, no prosegment and no potential N-linked glycosylation site(s) (1, 2). It is likely that mouse IL-1F8, similarly to its human homologue, is actively secreted (1). Mouse IL-1F8 shares 61% and 74% aa identity with human IL-1F8 isoform 2 and rat IL-1F8, respectively. Within the IL-1 family, IL-1F8 shares 27% sequence with 33%, 32%, 35%, 39% and 28% aa sequence identity with IL-1 ra, IL-1 beta, IL-1F5, F6, F9 and F10, respectively. Cells reported to express IL-1F8 include resting and activated monocytes and B cells (1, 4). The receptor for IL-1F8 is reported to be a combination of IL-1 Rrp2 and IL-1 RAcP (5). Recombinant IL-1F8, along with IL-1F6 and IL-1F9, has been shown to activate the pathway involving NF-kappa B and MAPK in an IL-1Rrp2 dependent manner.

Long Name
Interleukin 36 beta/Interleukin 1 Family 8
Entrez Gene IDs
27177 (Human); 69677 (Mouse)
Alternate Names
family of interleukin 1-eta; FIL1 eta; FIL1; FIL1-(ETA); FIL1H; FILI-(ETA); IL-1 eta; IL1-ETA; IL1F8 (Canonical product IL-1F8a); IL-1F8 (FIL1-eta); IL1F8; IL-1F8; IL1H2; IL-1H2; IL36 beta; IL-36 beta; IL36B; interleukin 1 family, member 8 (eta); interleukin 1, eta; Interleukin 36, Beta; Interleukin-1 eta; interleukin-1 family member 8; Interleukin-1 homolog 2; Interleukin-1 Superfamily e; Interleukin-36 Beta

Product Datasheets

Citation for Mouse IL-36 beta/IL-1F8 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. The new IL-1 family member IL-1F8 stimulates production of inflammatory mediators by synovial fibroblasts and articular chondrocytes.
    Authors: Magne D, Palmer G, Barton JL, Mezin F, Talabot-Ayer D, Bas S, Duffy T, Noger M, Guerne PA, Nicklin MJ, Gabay C
    Arthritis Res. Ther., 2006;8(3):R80.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: ELISA Development

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