Mouse IL-3R beta Antibody

Catalog # Availability Size / Price Qty
MAB5491
MAB5491-SP
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Mouse IL-3R beta Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse IL‑3 R beta in ELISAs. In sandwich immunoassays, no cross-reactivity or interference with recombinant mouse IL‑3 R alpha or recombinant human IL-3 R alpha is observed. Cross-reactivity with AIC2B was not tested.
Source
Monoclonal Rat IgG2A Clone # 130710
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample

Mouse IL-3 R beta Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
2-8 µg/mL 

Use in combination with:

Detection Reagent: Mouse IL-3R beta Biotinylated Antibody (Catalog # BAF549)

Standard: Recombinant Mouse IL-3R beta Fc Chimera Protein, CF (Catalog # 549-R3)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta  subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta  subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

References
  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.
Long Name
Interleukin 3 Receptor beta
Entrez Gene IDs
12984 (Mouse)
Alternate Names
AIC2A; BetaIl3; Bil3; Csfgmrb; IL3R beta; IL-3R beta; IL3R; IL3RB; IL-3Rb; Il3rb2

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