Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 130714
View all IL-3R beta Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse IL-3 R beta
His23-Trp440
Accession # P26954

Specificity

Detects mouse IL-3 R beta in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Applications for Mouse IL-3R beta Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Mouse IL‑3 R beta Fc Chimera (Catalog # 549-R3)

Reviewed Applications

Read 1 review rated 4 using MAB549 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-3R beta

Interleukin 3 (IL-3) is a pleiotropic cytokine produced primarily by activated T cells or mast cells. IL-3 stimulates the proliferation and differentiation of hemopoietic cells including the pluripotent hematopoietic stem cells as well as various lineage-committed cells. The biological effects of IL-3 on the various cell types are mediated by the binding of IL-3 to specific cell surface receptor complexes. The functional high-affinity IL-3 receptor is a heterodimer consisting of a ligand binding alpha subunit and the beta subunit. The alpha subunit alone binds IL-3 with low affinity. The beta subunit is required for the high-affinity binding of IL-3 to the heterodimeric receptor complex. The beta subunit has also been found to be a component of the high-affinity receptor complex for IL-5 and GM-CSF and is also referred to as the beta common ( beta c) chain. In the mouse, there are two IL-3 R beta proteins. The first identified mouse IL-3 R beta was also called AIC2A and binds IL-3 with low affinity (1). The second mIL-3 R beta was referred to as AIC2B (2). AIC2B doesn’t bind IL-3 and is the homolog of the human IL-3 R beta. AIC2A was found to be the result of a gene duplication event. Both the alpha  and the beta  subunits are members of the cytokine receptor superfamily (3).

References

  1. Itoh, N. et al. (1990) Science 247:324.
  2. Gorman, D.M. et al. (1990) Proc. Natl. Acad. Sci. USA 87:5459.
  3. Schrader, J.W. in Cytokine Reference, (2001) J.J. Oppenheim and M. Feldmann, eds. Academic Press, New York, p. 1899.

Long Name

Interleukin 3 Receptor beta

Alternate Names

IL-3R beta, IL-3Rb, IL3R beta

Entrez Gene IDs

12984 (Mouse)

Gene Symbol

CSF2RB2

UniProt

P26954

Additional IL-3R beta Products

Product Documents for Mouse IL-3R beta Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse IL-3R beta Antibody

For research use only

Customer Reviews for Mouse IL-3R beta Antibody (1)

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  • Mouse IL-3 R beta Antibody
    Name: Anonymous
    Application: Simple Western
    Sample Tested: Mouse Spleen Lysate
    Species: Mouse
    Verified Customer | Posted 06/07/2019
    Mouse spleen lysate (at the indicated concentrations) was probed with 40 µg/mL of monoclonal antibody. As a control for the anti-rat/HRP secondary, 200 ng/mL of the monoclonal IgG was run in the indicated lane.
    Mouse IL-3R beta Antibody MAB549

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