Mouse IL-5 R alpha/CD125 Biotinylated Antibody

Catalog # Availability Size / Price Qty

SAVE +FREE SHIPPING with ACADEMIA23 (See Promo Details)

Product Details
Supplemental Products

Mouse IL-5 R alpha/CD125 Biotinylated Antibody Summary

Species Reactivity
Detects mouse IL‑5 R alpha /CD125 in Western blots. In Western blots, approximately 10% cross-reactivity with recombinant human (rh) IL-5 R alpha is observed and less than 1% cross-reactivity with recombinant mouse (rm) IL-4 R, rhIL-9 R, rhIL-13 R alpha 1, and rmIL-13 R alpha 2 is observed.
Polyclonal Goat IgG
Antigen Affinity-purified
Mouse myeloma cell line NS0-derived recombinant mouse IL‑5 R alpha /CD125
Accession # P21183
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.


Recommended Concentration
Western Blot
0.1 µg/mL
Recombinant Mouse IL‑5 R alpha /CD125

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Size / Price
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-5 R alpha/CD125

Interleukin‑5 Receptor alpha (IL‑5 R alpha ), also known as CD125, is a 60 kDa hematopoietin receptor that plays a dominant role in eosinophil biology (1‑3). Mature mouse IL‑5 R alpha consists of a 322 amino acid (aa) extracellular domain (ECD) with a WSxWS motif and a four cysteine motif, a 22 aa transmembrane segment, and a 54 aa cytoplasmic domain (4). Within the ECD, mouse IL‑5 R alpha shares 71% and 86% aa sequence identity with human and rat IL‑5 R alpha, respectively. Alternate splicing of mouse IL‑5 R alpha generates soluble secreted forms which function as IL‑5 antagonists (4, 5). The high affinity receptor for IL‑5 is a complex that consists of the ligand binding IL‑5 R alpha and the transmembrane common  beta chain ( beta c/CD131) which is shared with the receptor complexes for IL‑3 and GM‑CSF (6). IL‑5 R alpha binds IL‑5 at low affinity and then associates with preformed beta c oligomers to form the signaling‑competent receptor complex (7). IL‑5 stimulation of CD34+ hematopoietic progenitor cells induces the up‑regulation of transmembrane IL‑5 R alpha followed by eosinophilic differentiation and activation (8‑10). IL‑5 R alpha also promotes the differentiation of basophils and B cells (11, 12). Exposure of mature eosinophils to IL‑5 attenuates their IL‑5 responsiveness by inducing the down‑regulation of surface IL‑5 R alpha and increased production of soluble IL‑5 R alpha (13, 14). Elevated production of IL‑5 at sites of allergic inflammation induces eosinophilia and exacerbation of immune cell infiltration, tissue damage, and remodeling (2, 3).

Long Name
Interleukin 5 Receptor alpha
Entrez Gene IDs
3568 (Human); 16192 (Mouse)
Alternate Names
CD125 antigen; CD125; CDw125CD125; HSIL5R3; IL-5 R alpha; IL-5 receptor subunit alpha; IL5R alpha; IL-5R subunit alpha; IL5R; IL5RA; IL-5Ra; IL-5R-alpha; interleukin 5 receptor type 3; interleukin 5 receptor, alpha; interleukin-5 receptor alpha chain; interleukin-5 receptor alpha subunit; interleukin-5 receptor subunit alpha; MGC26560

Product Datasheets

You must select a language.



No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse IL-5 R alpha/CD125 Biotinylated Antibody

There are currently no reviews for this product. Be the first to review Mouse IL-5 R alpha/CD125 Biotinylated Antibody and earn rewards!

Have you used Mouse IL-5 R alpha/CD125 Biotinylated Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review