Mouse IL-7 Quantikine ELISA Kit

Catalog # Availability Size / Price Qty
M7000
Mouse IL-7 ELISA Standard Curve
2 Images
Product Details
Procedure
Citations (4)
FAQs
Supplemental Products
Reviews (10)

Mouse IL-7 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (50 uL), EDTA Plasma (50 uL)
Sensitivity
8.3 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma)
Specificity
Natural and recombinant mouse IL-7
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Mouse IL-7 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse IL-7 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse IL-7 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse IL-7 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse IL-7.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, EDTA Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 41 41 41
Mean 82 211 819 84 215 770
Standard Deviation 3.5 5.9 29.6 6.7 16.6 67.1
CV% 4.3 2.8 3.6 8 7.7 8.7

Recovery

The recovery of mouse IL-7 spiked to three levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Supernates (n=6) 110 107-113
EDTA Plasma (n=4) 109 100-118
Serum (n=4) 108 96-120

Linearity

To assess the linearity of the assay, samples spiked with high concentrations of mouse IL-7 in each matrix were diluted with Calibrator Diluent and then assayed.
Mouse IL-7 ELISA Linearity

Data Examples

Mouse IL-7 ELISA Standard Curve

Product Datasheets

Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: IL-7

IL-7 (Interleukin-7) is a cytokine that plays important roles in lymphocyte differentiation, proliferation, and survival. IL-7 is produced by stromal epithelial cells of the thymus, bone marrow, and intestines. It signals through a receptor complex composed of IL-7 R alpha/CD127 and the Common gamma Chain. gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21. IL-7 contributes to the maintenance of all naïve and memory T cells. It is required for optimal T cell-dendritic cell interaction. In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development.

Long Name:
Interleukin 7
Entrez Gene IDs:
3574 (Human); 16196 (Mouse); 25647 (Rat)
Alternate Names:
IL7; IL-7; IL-7interleukin-7; interleukin 7; Lymphopoietin-1; PBGF
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 50 µL Assay Diluent
  4.   Add 50 µL of Assay Diluent to each well.

  5. 50 µL Standard, Control, or Sample
  6.   Add 50 µL of Standard, Control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 4 times for a total of 5 washes.

  8. 100 µL Conjugate
  9.   Add 100 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 5 times.

  11. 100 µL Substrate Solution
  12.   Add 100 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 100 µL Stop Solution
  14.   Add 100 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Mouse IL-7 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. All-trans retinoid acid promotes allogeneic corneal graft survival in mice by regulating Treg-Th17 balance in the presence of TGF-beta.
    Authors: Wang X, Wang W, Xu J, Wu S, Le Q
    BMC Immunol, 2015;16(0):17.
    Species: Mouse
    Sample Types: Serum
  2. Innate immune cell CD45 regulates lymphopenia-induced T cell proliferation.
    Authors: Saunders A, Shim Y, Johnson P
    J Immunol, 2014;193(6):2831-42.
    Species: Mouse
    Sample Types: Tissue Homogenates
  3. IL-7 promotes T cell viability, trafficking, and functionality and improves survival in sepsis.
    Authors: Unsinger J, McGlynn M, Kasten KR, Hoekzema AS, Watanabe E, Muenzer JT, McDonough JS, Tschoep J, Ferguson TA, McDunn JE, Morre M, Hildeman DA, Caldwell CC, Hotchkiss RS
    J. Immunol., 2010;184(7):3768-79.
    Species: Human
    Sample Types: Plasma
  4. Osteoblasts support B-lymphocyte commitment and differentiation from hematopoietic stem cells.
    Authors: Zhu J, Garrett R, Jung Y, Zhang Y, Kim N, Wang J, Joe GJ, Hexner E, Choi Y, Taichman RS, Emerson SG
    Blood, 2007;109(9):3706-12.
    Species: Mouse
    Sample Types: Cell Culture Supernates

FAQs

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Reviews for Mouse IL-7 Quantikine ELISA Kit

Average Rating: 4.9 (Based on 10 Reviews)

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Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 05/30/2018
Application: Sample Tested: Serum,Lymph node cells

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 04/13/2018
Application: Sample Tested: Pancreas tissue,Plasma

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 04/12/2018
Application: Sample Tested: BW5147 mouse T lymphoma cell line

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 03/30/2018
Application: Sample Tested: 3T3-L1 mouse embryonic fibroblast adipose-like cell line

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 03/14/2018
Application: Sample Tested: E4.5 mouse embryo

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 03/08/2018
Application: Sample Tested: Serum-free Cell Culture Supernates

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 03/07/2018
Application: Sample Tested: 4T1 mouse breast cancer cell line

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 02/17/2018
Application: Sample Tested: 4T1 mouse breast cancer cell line

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 02/14/2018
Application: Sample Tested: Cell culture supernatant

Mouse IL-7 Quantikine ELISA Kit
By Anonymous on 02/08/2018
Application: Sample Tested: Serum