Mouse Integrin  beta 4/CD104 Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.
  • Source
    Monoclonal Rat IgG2A Clone # 308601
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
    Asn29-Ser711
    Accession # NP_001005608
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    See below
  • Immunohistochemistry
    8-25 µg/mL
    Perfusion fixed frozen sections of mouse intestinal villi
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse Integrin beta 4/CD104 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin beta 4/CD104 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Integrin  beta 4/CD104 in CMT-93 Mouse Cell Line by Flow Cytometry. CMT‑93 mouse rectal carcinoma cell line was stained with Mouse Integrin  beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin  alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.
  • Entrez Gene IDs:
    3691 (Human); 192897 (Mouse); 25724 (Rat)
  • Alternate Names:
    CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4
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