Mouse Integrin  beta 4/CD104 Antibody

Catalog # Availability Size / Price Qty
MAB4054
MAB4054-SP
Detection of Mouse Integrin  beta 4/CD104 by Western Blot.
2 Images
Product Details
FAQs
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Reviews (4)

Mouse Integrin  beta 4/CD104 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 4/CD104 in direct ELISAs and Western blots. Does not cross-react with recombinant mouse Integrin beta 1, beta 2, beta 6, beta 7, or recombinant human Integrin beta 3.
Source
Monoclonal Rat IgG2A Clone # 308601
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin  beta 4/CD104
Asn29-Ser711
Accession # NP_001005608
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
2 µg/mL
See below
Flow Cytometry
2.5 µg/106 cells
See below
Immunohistochemistry
8-25 µg/mL
Perfusion fixed frozen sections of mouse intestinal villi
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse Integrin  beta 4/CD104 antibody by Western Blot. View Larger

Detection of Mouse Integrin beta 4/CD104 by Western Blot. Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin beta 4/CD104 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry Detection of Integrin  beta 4/CD104 antibody in CMT-93 Mouse Cell Line antibody by Flow Cytometry. View Larger

Detection of Integrin beta 4/CD104 in CMT-93 Mouse Cell Line by Flow Cytometry. CMT-93 mouse rectal carcinoma cell line was stained with Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2Secondary Antibody (Catalog # F0105B).

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 4/CD104

Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM) glycoprotein that associates only with Integrin  alpha 6/CD29. Integrin alpha 6 beta 4 is predominantly expressed by epithelial cells, binds laminins, and is essential for formation of hemidesmosomes and connection of the dermis to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa) extracellular domain (ECD) with a metal-binding site and four cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa cytoplasmic tail that organizes hemidesmosome components. Isoforms with shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD show 88% aa identity.

Entrez Gene IDs
3691 (Human); 192897 (Mouse); 25724 (Rat)
Alternate Names
CD104 antigen; CD104; GP150; Integrin beta 4; integrin beta-4 subunit; integrin beta-4; integrin, beta 4; ITGB4

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Reviews for Mouse Integrin  beta 4/CD104 Antibody

Average Rating: 4.5 (Based on 4 Reviews)

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Mouse Integrin  beta 4/CD104 Antibody
By Anonymous on 08/20/2022
Application: IHC Sample Tested: Small intestine tissue Species: Mouse

Mouse Integrin  beta 4/CD104 Antibody
By Anonymous on 11/30/2021
Application: IHC Sample Tested: Prostate tissue Species: Mouse

Mouse Integrin  beta 4/CD104 Antibody
By Anonymous on 12/13/2019
Application: MiAr Sample Tested: EDTA Plasma Species: Human

Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma


Mouse Integrin  beta 4/CD104 Antibody
By Anonymous on 11/10/2018
Application: ELISA Sample Tested: Plasma Species: Human