Detection of Mouse Integrin beta 4/CD104 by Western Blot.
Western blot shows lysates of mouse small intestine tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Integrin beta 4/CD104 at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Integrin beta 4/CD104 in CMT-93 Mouse Cell Line by Flow Cytometry.
CMT‑93 mouse rectal carcinoma cell line was stained with Mouse Integrin beta 4/CD104 Monoclonal Antibody (Catalog # MAB4054, filled histogram) or isotype control antibody (Catalog # MAB006, open histogram), followed by Phycoerythrin-conjugated Anti-Rat IgG F(ab')2 Secondary Antibody (Catalog # F0105B).
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Integrin beta 4/CD104
Integrin beta 4, also known as CD104, is a 200 kDa type I transmembrane (TM)
glycoprotein that associates only with Integrin alpha 6/CD29. Integrin alpha 6 beta 4
is predominantly expressed by epithelial cells, binds laminins, and is
essential for formation of hemidesmosomes and connection of the dermis
to the epidermis. Mouse integrin beta 4 contains a 680 amino acid (aa)
extracellular domain (ECD) with a metal-binding site and four
cysteine-rich repeats, a 22 aa TM segment and an unusually long 1073 aa
cytoplasmic tail that organizes hemidesmosome components. Isoforms with
shortened cytoplasmic tails have been described. Mouse and human beta 4 ECD
show 88% aa identity.
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