Mouse Integrin  beta 7 Antibody

Catalog # Availability Size / Price Qty
MAB30601
MAB30601-SP
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Mouse Integrin  beta 7 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Integrin beta 7 extracellular domain in direct ELISAs.
Source
Monoclonal Rat IgG2A Clone # 332408
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse Integrin beta 7
Glu20-His722
Accession # P26011
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Adhesion Activation
The adhesion of TK-1 mouse T cell lymphoma cells (5 x 104 cells/well, treated with 1 mM MnCl2) to immobilized Recombinant Mouse MAdCAM-1 Fc Chimera (Catalog # 993-MC, 1 µg/well, 100 µL/well) was maximally enhanced (2‑2.5 fold) by 10 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 7

Integrin beta 7 associates with the integrin alpha 4 or alpha E subunits to form heterodimers to interact with fibronectin, VCAM, MAdCAM or E-Cadherin. It is highly expressed in leukocytes and is involved in localization of leukocytes at mucosal sites.

Entrez Gene IDs
3695 (Human); 16421 (Mouse); 25713 (Rat); 102137082 (Cynomolgus Monkey)
Alternate Names
Gut homing receptor beta subunit; integrin beta 7 subunit; Integrin beta 7; integrin beta-7; integrin, beta 7; ITGB7

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