Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human LAG-3 is observed.
Monoclonal Rat IgG2B Clone # 631501
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with mouse LAG-3
Accession # Q61790
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 488
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 3 days with PMA and Calcium Ionomycin and then stained with Rat Anti-Mouse LAG‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB33281G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
Related Research Areas
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