Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were treated for 3 days with PMA and Calcium Ionomycin and then stained with Rat Anti-Mouse LAG‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB33281G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
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