Mouse LAG-3 Alexa Fluor® 488-conjugated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 631501
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse LAG-3
    Ser23-Leu442
    Accession # Q61790
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of LAG‑3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 3 days with PMA and Calcium Ionomycin and then stained with Rat Anti-Mouse LAG‑3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB33281G, filled histogram) or isotype control antibody (Catalog # IC013G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAG-3
LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
  • Long Name:
    Lymphocyte-activation Gene 3
  • Entrez Gene IDs:
    3902 (Human); 16768 (Mouse); 297596 (Rat); 102122272 (Cynomolgus Monkey)
  • Alternate Names:
    CD223 antigen; CD223; FDC; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 488-conjugated Isotype Control

Ctrl IC013G
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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