Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human LAG-3 is observed.
Monoclonal Rat IgG2B Clone # 631501
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with mouse LAG-3
Accession # Q61790
Supplied in a saline solution containing BSA and Sodium Azide.
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
LAG-3 (Lymphocyte activation gene-3; also known as CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
Related Research Areas
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