Mouse LAG‑3 PE‑conjugated Antibody

  • Specificity
    Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human LAG-3 is observed.
  • Source
    Monoclonal Rat IgG2B Clone # 631501
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    NS0 mouse myeloma cell line transfected with mouse LAG-3
    Accession # Q61790
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.

Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAG-3
LAG-3 (Lymphocyte activation gene-3; also known as CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity.  The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
  • Long Name:
    Lymphocyte-activation Gene 3
  • Alternate Names:
    CD223; CD223; CD223 antigen; FDC; LAG-3; lymphocyte activation gene 3 protein; lymphocyte-activation gene 3; Protein FDC
Related Research Areas


No product specific FAQs exist for this product, however you may click here to view all FAQs for Primary Antibodies

Order Details

Contact Information

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest


Customer Information