Mouse LAG‑3 PE‑conjugated Antibody
R&D Systems | Catalog # FAB33281P
Key Product Details
Validated by
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser23-Leu442
Accession # Q61790
Specificity
Clonality
Host
Isotype
Scientific Data Images for Mouse LAG‑3 PE‑conjugated Antibody
Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes treated with PMA and Calcium Ionomycin for 3 days were stained with Rat Anti-Mouse LAG-3 PE-conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.
Detection of LAG‑3 in CTLL‑2 mouse cytotoxic T cell line by Flow Cytometry
CTLL‑2 mouse cytotoxic T cell line were stained with Rat Anti-Mouse LAG‑3 PE‑conjugated Monoclonal Antibody (Catalog # FAB33281P, filled histogram) or isotype control antibody (Catalog # IC013P, open histogram). View our protocol for Staining Membrane-associated Proteins.Applications for Mouse LAG‑3 PE‑conjugated Antibody
Flow Cytometry
Sample: Mouse splenocytes treated with PMA and Calcium Ionomycin
Spectra Viewer
Plan Your Experiments
Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.
Spectra Viewer
Flow Cytometry Panel Builder
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Advanced Features
- Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
- Spillover Popups - Visualize the spectra of individual fluorochromes
- Antigen Density Selector - Match fluorochrome brightness with antigen density
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, 2 to 8 °C as supplied.
Background: LAG-3
Long Name
Alternate Names
Gene Symbol
UniProt
Additional LAG-3 Products
Product Documents for Mouse LAG‑3 PE‑conjugated Antibody
Certificate of Analysis
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Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse LAG‑3 PE‑conjugated Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- 7-Amino Actinomycin D (7-AAD) Cell Viability Flow Cytometry Protocol
- Extracellular Membrane Flow Cytometry Protocol
- Flow Cytometry Protocol for Cell Surface Markers
- Flow Cytometry Protocol for Staining Membrane Associated Proteins
- Flow Cytometry Staining Protocols
- Flow Cytometry Troubleshooting Guide
- Intracellular Flow Cytometry Protocol Using Alcohol (Methanol)
- Intracellular Flow Cytometry Protocol Using Detergents
- Intracellular Nuclear Staining Flow Cytometry Protocol Using Detergents
- Intracellular Staining Flow Cytometry Protocol Using Alcohol Permeabilization
- Intracellular Staining Flow Cytometry Protocol Using Detergents to Permeabilize Cells
- Propidium Iodide Cell Viability Flow Cytometry Protocol
- Protocol for Liperfluo
- Protocol for the Characterization of Human Th22 Cells
- Protocol for the Characterization of Human Th9 Cells
- Protocol: Annexin V and PI Staining by Flow Cytometry
- Protocol: Annexin V and PI Staining for Apoptosis by Flow Cytometry
- Troubleshooting Guide: Fluorokine Flow Cytometry Kits
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
FAQs for Mouse LAG‑3 PE‑conjugated Antibody
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Q: Can this LAG-3 antibody be used in ELISA?
A: We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
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Q: I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
A: There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.
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Q: Can this LAG-3 antibody be used in ELISA?
A: We have not validated this particular LAG-3 antibody for ELISA in mouse; however, it has been cited as being used as a detection antibody in a publicatoin (PMID 15946792).
-
Q: I'm using your LAG-3 antibody in western blot but the observed band is a different size than the predicted band size, do you know what the bands are?
A: There are several alternative splice varients of LAG-3 that could explain the different sized proteins stained by the LAG-3 antibody. It is also believed that there are two secreted forms of LAG-3, one that is about 36 kDa and another at 52 kDa.