Detects mouse LAG-3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human LAG-3 is observed.
Monoclonal Rat IgG2B Clone # 631501
Protein A or G purified from hybridoma culture supernatant
NS0 mouse myeloma cell line transfected with mouse LAG-3
Accession # Q61790
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
2.5 µg/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are
available in the Technical Information section on our website.
Detection of LAG-3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were treated for 72 hours with 50 ng/mL PMA and 500 ng/mL Ca2+ Ionomycin then stained with Rat Anti-Mouse LAG‑3 Monoclonal Antibody (Catalog # MAB33281, filled histogram) or isotype control antibody (Catalog # MAB0061, open histogram), followed by Allophycocyanin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0113).
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
LAG-3 (Lymphocyte activation gene-3; CD223 in the human) is a member of the immunoglobulin superfamily (IgSF). The mature LAG-3 protein is a 496 amino acid (aa) membrane protein with a 421 aa extracellular region which contains four IgSF domains, a 21 aa transmembrane region and a 54 aa cytoplasmic region. LAG-3 shares < 20% amino acid sequence homology with CD4, but has similar structure and binds to MHC class II with higher affinity. The mouse LAG-3 extracellular region shares 69% aa sequence identity with human LAG-3.
Related Research Areas
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