Mouse Lgr5/GPR49 Alexa Fluor® 647-conjugated Antibody

    
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  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse Lgr5/GPR49 in direct ELISAs. Stains mouse Lgr5 transfected cells but not irrelevant transfectants in flow cytometry. This clone, also known as "RD20", has been found to detect an epitope in C-terminal LRR cap of LGR5 (Ref. 1).
  • Source
    Monoclonal Rat IgG2B Clone # 803420
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant mouse Lgr5/GPR49
    Lys375-Pro547
    Accession # Q9Z1P4
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 647
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.1 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
 Detection of Lgr5/GPR49 in HEK293 Human Cell Line Transfected with Mouse Lgr5/GPR49 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either (A) mouse Lgr5/GPR49 or (B) irrelevant transfectants and eGFP was stained with 0.1 μg Rat Anti-Mouse Lgr5/GPR49 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB8240R). Quadrant markers were set based on control antibody staining (Catalog # IC0041R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: Lgr5/GPR49
Leucine‑rich repeat G‑protein‑coupled receptor 5 (Lgr5), also called GPR49, is a 907 amino acid (aa), approximately 97 kDa (calculated), seven‑transmembrane glycoprotein receptor in the Lgr family of cell surface receptors. The subfamily of Lgrs comprising Lgr4, Lgr5, and Lgr6 are G‑protein‑independent mediators of the potentiating effect of R‑Spondins on Wnt signaling (2). Lgr5 binds and forms complexes with R‑Spondins, Frizzled Wnt receptors and LRP Wnt co‑receptors. The region of the mouse Lgr5 long extracellular domain used as an immunogen shares 90% and 95% amino acid sequence identity with human and rat Lgr5, respectively. Lgr5 is found on embryonic and adult epithelial stem cells (3). Lgr5+ stem cells can produce all epithelial cell types of the intestinal crypts (4). It is upregulated in stem cells that give rise to cancers such as intestinal, hepatocellular, pancreatic and ovarian carcinomas (5,6). This antibody has been referred to as "RD20" by Peng et al.(1).
  • References:
    1. Peng et al. (2013) Cell Rep. 3(6):1885.
    2. de Lau et al. (2014) Genes Dev. 28(4):305.
    3. Barker et al. (2013) Development 140(12):2484.
    4. Clevers (2013) Nature 495(7439):53.
    5. Wu et al. (2014) Nat Commun 5:3149.
    6. Jang et al. (2013) PLoS One 8(12):e82390495.
  • Long Name:
    Leucine-rich Repeat Containing G Protein-coupled Receptor 5
  • Entrez Gene IDs:
    8549 (Human); 14160 (Mouse); 299802 (Rat)
  • Alternate Names:
    FEX; G protein-coupled receptor 49; GPR49; GPR49G-protein coupled receptor HG38; GPR67; G-protein coupled receptor 49; G-protein coupled receptor 67; GRP49; HG38; leucine-rich repeat containing G protein-coupled receptor 5; leucine-rich repeat-containing G protein-coupled receptor 5; leucine-rich repeat-containing G-protein coupled receptor 5; Lgr5; MGC117008; orphan G protein-coupled receptor HG38
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Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 647-conjugated Isotype Control

Ctrl IC013R
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Staining Reagents
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Flow Cytometry Staining Buffer (1X)

Flow FC001 7
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 5
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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