Mouse Lipin 3 is a 94 kDa member of the small Lipin family of nuclear proteins. It is primarily expressed in the GI tract and may play a role in DAG production or membrane synthesis. Lipin 3 is 848 amino acids (aa) in length. It shows conserved N- and C-terminal domains that flank an NLS between aa 135‑144. Lipin 3 has modest phosphatidate phosphatase activity. Two potential isoforms exist; one shows a deletion of aa 507‑537, while the other shows a 10 aa insertion between Glu 506 and Ser 507. Over aa 428‑549, mouse Lipin 3 shares 84% and 96% aa sequence identity with human and rat Lipin 3, respectively.
Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody
R&D Systems | Catalog # AF4064AFP680
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry
Label
Alexa Fluor Plus 680 (Excitation = 687 nm, Emission = 704 nm)
Antibody Source
Polyclonal Goat IgG
Loading...
Product Specifications
Specificity
Detects mouse Lipin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Lipin 3 is observed and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2 is observed.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Applications for Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody
Application
Recommended Usage
Immunocytochemistry
Optimal dilution of this antibody should be experimentally determined.
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Lipin 3
Long Name
Phosphatidate Phosphatase LPIN3
Alternate Names
LIPN3L, LPIN3, SMP2
Gene Symbol
LPIN3
UniProt
Additional Lipin 3 Products
Product Documents for Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
Related Research Areas
Customer Reviews
There are currently no reviews for this product. Be the first to review Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody and earn rewards!
Have you used Mouse Lipin 3 Alexa Fluor™ Plus 680‑conjugated Antibody?
Submit a review and receive an Amazon gift card!
$25/€18/£15/$25CAN/¥2500 Yen for a review with an image
$10/€7/£6/$10CAN/¥1110 Yen for a review without an image
Submit a review
Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
Loading...