Mouse Lipin 3 Antibody

Catalog # Availability Size / Price Qty
AF4064
AF4064-SP
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Mouse Lipin 3 Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse Lipin 3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 50% cross-reactivity with recombinant human (rh) Lipin 3 is observed and less than 5% cross-reactivity with rhLipin 1 and rhLipin 2 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant mouse Lipin 3
Thr428-Arg529
Accession # Q99PI4
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Mouse Lipin 3
Immunocytochemistry
5-15 µg/mL
Immersion fixed CCD-18co human colon fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipin 3

Mouse Lipin 3 is a 94 kDa member of the small Lipin family of nuclear proteins. It is primarily expressed in the GI tract and may play a role in DAG production or membrane synthesis. Lipin 3 is 848 amino acids (aa) in length. It shows conserved N- and C-terminal domains that flank an NLS between aa 135‑144. Lipin 3 has modest phosphatidate phosphatase activity. Two potential isoforms exist; one shows a deletion of aa 507‑537, while the other shows a 10 aa insertion between Glu 506 and Ser 507. Over aa 428‑549, mouse Lipin 3 shares 84% and 96% aa sequence identity with human and rat Lipin 3, respectively.

Long Name
Phosphatidate Phosphatase LPIN3
Entrez Gene IDs
64900 (Human); 64899 (Mouse); 362261 (Rat)
Alternate Names
dJ450M14.2; dJ450M14.3; dJ620E11.2; EC 3.1.3.4; Lipin 3; lipin 3-like; lipin-3; Lipin-3-like; LIPN3L; LPIN3; phosphatidate phosphatase LPIN3; SMP2

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