Mouse LRRC32/GARP Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB62291G Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
FAB62291G-100UG
Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry.
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Mouse LRRC32/GARP Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity
Mouse
Specificity
Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) LRRC32 is observed and no cross-reactivity with rhLRRC3, rhLRRC4, or rhNGL-3/LRRC4B is observed.
Source
Monoclonal Rat IgG1 Clone # 725226
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP
Ile18-Asn628
Accession # NP_001106850
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Label
Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25-1 µg/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of LRRC32/GARP antibody in Mouse Splenocytes antibody by Flow Cytometry. View Larger

Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stimulated with anti-CD3/anti-CD28, Mouse Recombinant IL-2 (Catalog # 402-ML), and TGF-beta for 3 days to induce regulatory T cell differentiation. Cells were stained with Rat Anti-Mouse CD4 PerCP-conjugated Monoclonal Antibody (Catalog # FAB554C) and either (A) Rat Anti-Mouse LRRC32/GARP Alexa Fluor® 488-conjugated Monoclonal Antibody (Catalog # FAB62291G) or (B) Rat IgG1Alexa Fluor 488 Isotype Control (Catalog # IC005G). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: LRRC32/GARP

Leucine-rich Repeat Protein 32 (LRRC32), also known as GARP (Glycoprotein A Repetitions Predominant), is an 77-80 kDa type I transmembrane glycoprotein. Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, followed by a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain. Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is expressed on hepatic stellate cells and on adult platelets. Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg). LRRC32 expression promotes the acquisition of a Treg phenotype that is characterized by reduced cellular proliferation and cytokine secretion, plus the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and, in association with alpha V beta 8 Integrin, mediates the activation and release of TGF-beta  from the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.

Long Name
Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
Entrez Gene IDs
2615 (Human); 434215 (Mouse)
Alternate Names
D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32

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Product Specific Notices


This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

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