Detects mouse LRRC32/GARP in direct ELISAs. In direct ELISAs, approximately 50% cross-reactivity
with recombinant human (rh) LRRC32 is observed and no cross-reactivity with
rhLRRC3, rhLRRC4, or rhNGL-3/LRRC4B is observed.
Monoclonal Rat IgG1 Clone # 725226
Protein A or G purified from hybridoma culture supernatant
Chinese hamster ovary cell line CHO-derived recombinant mouse LRRC32/GARP Ile18-Asn628 Accession # NP_001106850
Supplied in a saline solution containing BSA and Sodium Azide.
Alexa Fluor 700
5 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of LRRC32/GARP in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stimulated with anti-CD3/anti-CD28, Mouse Recombinant IL-2 (Catalog # 402-ML), and TGF-beta for 3 days to induce regulatory T cell differentiation. Cells were stained with Rat Anti-Mouse CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB554F) and either (A) Rat Anti-Mouse LRRC32/GARP Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB62291N) or (B) Rat IgG1 Alexa Fluor 700 Isotype Control (Catalog # IC005N). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Leucine-rich Repeat Protein 32 (LRRC32), also known as GARP (Glycoprotein A Repetitions Predominant), is an 77-80 kDa type I transmembrane glycoprotein. Mature mouse LRRC32 consists of a 608 amino acid (aa) extracellular domain (ECD) that contains 22 leucine-rich repeats, followed by a 21 aa transmembrane segment, and a 14 aa cytoplasmic domain. Within the ECD, mouse LRRC32 shares 80 and 94% aa sequence identity with human and rat LRRC32, respectively. LRRC32 is expressed on hepatic stellate cells and on adult platelets. Among T cells, it is selectively expressed on activated FOXP3+ regulatory T cells (Treg). LRRC32 expression promotes the acquisition of a Treg phenotype that is characterized by reduced cellular proliferation and cytokine secretion, plus the capacity to suppress the proliferation of naïve T cells. LRRC32 binds directly to the TGF-beta latency associated peptide (LAP) and, in association with alpha V beta 8 Integrin, mediates the activation and release of TGF-beta from the surface of activated Treg cells. The presentation of TGF-beta on Tregs contributes to their ability to suppress naïve T cell proliferation.
Leucine-rich Repeat Containing 32/Glycoprotein A Repetitions Predominant
Entrez Gene IDs:
2615 (Human); 434215 (Mouse)
D11S833E; D11S833Eleucine-rich repeat-containing protein 32; GARP; GARPGarpin; Garpin; Glycoprotein A repetitions predominantgarpin; leucine rich repeat containing 32; LRRC32
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