Reacts with mouse CD4, an antigen co-receptor on the T cell surface which interacts with MHC II molecules on antigen presenting cells.
Monoclonal Rat IgG2B Clone # GK1.5
Protein A or G purified from hybridoma culture supernatant
Mouse CTL clone V4
Supplied in a saline solution containing BSA and Sodium Azide.
PerCP (Peridinin-chlorophyll Protein Complex)
10 µL/106 cells
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols
are available in the Technical Information section on our website.
Detection of CD4 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse CD3 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB4841G) and either (A) Rat Anti-Mouse CD4 PerCP‑conjugated Monoclonal Antibody (Catalog # FAB554C) or (B) Rat IgG2B PerCP Isotype Control (Catalog # IC013C). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
CD4 is a type I membrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed predominantly on thymocytes and a subset of mature T lymphocytes. CD4 functions in collaboration with the T cell receptor in the recognition of peptide antigens that are presented by class II major histocompatibility complexes. CD4 also has been shown to be a coreceptor of HIV entry and specifically binds gp120, the external envelope glycoprotein of HIV.
Entrez Gene IDs:
920 (Human); 12504 (Mouse); 24932 (Rat); 403931 (Canine); 493775 (Feline)
CD4; CD4 antigen; CD4 antigen (p55); CD4 molecule; CD4 receptor; CD4mut; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4
Related Research Areas