Mouse M-CSF R/CD115 Biotinylated Antibody

(1 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse M-CSF R/CD115 in Western blots. In Western blots, approximately 25% cross-reactivity with recombinant human
    M-CSF R/CD115 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse M-CSF R/CD115
    Ala20-Ser511
    Accession # P09581
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse M-CSF R/CD115 Fc Chimera (Catalog # 3818-MR)
  • Flow Cytometry
    0.25 µg/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of M-CSF R/CD115 in RAW 264.7 Mouse Cell Line by Flow Cytometry.

RAW 264.7 mouse monocyte/macrophage cell line was stained with Sheep Anti-Mouse M-CSF R/CD115 Biotinylated Antigen Affinity‑purified Polyclonal Antibody (Catalog # BAF3818, filled histogram) or control antibody (Catalog # BAF020, open histogram), followed by Streptavidin-Phycoerythrin (Catalog # F0040).

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1-4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Mouse M-CSF receptor cDNA encodes a 977 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 492 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 441 aa cytoplasmic domain. The mouse M-CSF R ECD shares >99% aa identity with rat and 60-63% aa identity with corresponding sequences in human, canine, feline and bovine M-CSF R. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation, and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts, and DC (3, 4). M-CSF R and integrin alpha v beta 3 share signaling pathways during osteoclastogenesis, and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

  • References:
    1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.
    2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.
    3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.
    4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.
    5. Rovida, E. et al. (2001) J. Immunol. 166:1583.
    6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.
    7. Dai, X. et al. (2002) Blood 99:111.
    8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.
    9. Li, M. et al. (2004) J. Neurochem. 91:623.
    10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.
  • Long Name:
    Macrophage Colony Stimulating Factor Receptor
  • Entrez Gene IDs:
    1436 (Human)
  • Alternate Names:
    CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citations: Showing 1 - 1

  1. Fos plays an essential role in the upregulation of RANK expression in osteoclast precursors within the bone microenvironment.
    Authors: Arai A, Mizoguchi T, Harada S, Kobayashi Y, Nakamichi Y, Yasuda H, Penninger J, Yamada K, Udagawa N, Takahashi N
    J Cell Sci, 2012;125(0):2910-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
Isotype Controls
Description Application Cat# Citations Images  

Normal Sheep IgG Biotinylated Control

Ctrl BAF020 1
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Staining Reagents
Description Application Cat# Citations Images  

Streptavidin-Phycoerythrin

Flow F0040 7
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Streptavidin-Alkaline Phosphatase

ELISA(Det) AR001 2
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NorthernLights Streptavidin NL557

Flow, IHC, ICC NL999 4
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NorthernLights Streptavidin NL637

Flow, IHC, ICC NL998 2
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NorthernLights Streptavidin NL493

Flow, IHC, ICC NL997 1
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Streptavidin-Allophycocyanin

Flow F0050 1
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Avidin-Fluorescein

Flow F0030 1
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Supplemental Cell Selection Products
Description Application Cat# Citations Images  

MagCellect Streptavidin Ferrofluid

MAG999B 4
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MagCellect Streptavidin Ferrofluid

MAG999 4
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Supplemental ELISA Products
Description Application Cat# Citations Images  

Streptavidin-HRP

ELISA DY998 44
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EvenCoat Streptavidin Coated Plates

ELISA CP004
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EvenCoat Streptavidin Coated Plates, 5 Pack

ELISA CP003 3
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